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A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release

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A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release. / Brockmann, Marisa M.; Zarebidaki, Fereshteh; Camacho, Marcial; Grauel, M. Katharina; Trimbuch, Thorsten; Südhof, Thomas C.; Rosenmund, Christian.

In: CELL REP, Vol. 32, No. 5, 107960, 08.2020.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Brockmann, MM, Zarebidaki, F, Camacho, M, Grauel, MK, Trimbuch, T, Südhof, TC & Rosenmund, C 2020, 'A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release', CELL REP, vol. 32, no. 5, 107960. https://doi.org/10.1016/j.celrep.2020.107960

APA

Brockmann, M. M., Zarebidaki, F., Camacho, M., Grauel, M. K., Trimbuch, T., Südhof, T. C., & Rosenmund, C. (2020). A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release. CELL REP, 32(5), [107960]. https://doi.org/10.1016/j.celrep.2020.107960

Vancouver

Brockmann MM, Zarebidaki F, Camacho M, Grauel MK, Trimbuch T, Südhof TC et al. A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release. CELL REP. 2020 Aug;32(5). 107960. https://doi.org/10.1016/j.celrep.2020.107960

Bibtex

@article{aeed607c3d8d4340830779b06ee76839,
title = "A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release",
abstract = "At the presynaptic active zone, action-potential-triggered neurotransmitter release requires that fusion-competent synaptic vesicles are placed next to Ca2+ channels. The active zone resident proteins RIM, RBP, and Munc13 are essential contributors for vesicle priming and Ca2+-channel recruitment. Although the individual contributions of these scaffolds have been extensively studied, their respective functions in neurotransmission are still incompletely understood. Here, we analyze the functional interactions of RIMs, RBPs, and Munc13s at the genetic, molecular, functional, and ultrastructural levels in a mammalian synapse. We find that RBP, together with Munc13, promotes vesicle priming at the expense of RBP{\textquoteright}s role in recruiting presynaptic Ca2+ channels, suggesting that the support of RBP for vesicle priming and Ca2+-secretion coupling is mutually exclusive. Our results demonstrate that the functional interaction of RIM, RBP, and Munc13 is more profound than previously envisioned, acting as a functional trio that govern basic and short-term plasticity properties of neurotransmission.",
author = "Brockmann, {Marisa M.} and Fereshteh Zarebidaki and Marcial Camacho and Grauel, {M. Katharina} and Thorsten Trimbuch and S{\"u}dhof, {Thomas C.} and Christian Rosenmund",
year = "2020",
month = aug,
doi = "10.1016/j.celrep.2020.107960",
language = "English",
volume = "32",
journal = "CELL REP",
issn = "2211-1247",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - A Trio of Active Zone Proteins Comprised of RIM-BPs, RIMs, and Munc13s Governs Neurotransmitter Release

AU - Brockmann, Marisa M.

AU - Zarebidaki, Fereshteh

AU - Camacho, Marcial

AU - Grauel, M. Katharina

AU - Trimbuch, Thorsten

AU - Südhof, Thomas C.

AU - Rosenmund, Christian

PY - 2020/8

Y1 - 2020/8

N2 - At the presynaptic active zone, action-potential-triggered neurotransmitter release requires that fusion-competent synaptic vesicles are placed next to Ca2+ channels. The active zone resident proteins RIM, RBP, and Munc13 are essential contributors for vesicle priming and Ca2+-channel recruitment. Although the individual contributions of these scaffolds have been extensively studied, their respective functions in neurotransmission are still incompletely understood. Here, we analyze the functional interactions of RIMs, RBPs, and Munc13s at the genetic, molecular, functional, and ultrastructural levels in a mammalian synapse. We find that RBP, together with Munc13, promotes vesicle priming at the expense of RBP’s role in recruiting presynaptic Ca2+ channels, suggesting that the support of RBP for vesicle priming and Ca2+-secretion coupling is mutually exclusive. Our results demonstrate that the functional interaction of RIM, RBP, and Munc13 is more profound than previously envisioned, acting as a functional trio that govern basic and short-term plasticity properties of neurotransmission.

AB - At the presynaptic active zone, action-potential-triggered neurotransmitter release requires that fusion-competent synaptic vesicles are placed next to Ca2+ channels. The active zone resident proteins RIM, RBP, and Munc13 are essential contributors for vesicle priming and Ca2+-channel recruitment. Although the individual contributions of these scaffolds have been extensively studied, their respective functions in neurotransmission are still incompletely understood. Here, we analyze the functional interactions of RIMs, RBPs, and Munc13s at the genetic, molecular, functional, and ultrastructural levels in a mammalian synapse. We find that RBP, together with Munc13, promotes vesicle priming at the expense of RBP’s role in recruiting presynaptic Ca2+ channels, suggesting that the support of RBP for vesicle priming and Ca2+-secretion coupling is mutually exclusive. Our results demonstrate that the functional interaction of RIM, RBP, and Munc13 is more profound than previously envisioned, acting as a functional trio that govern basic and short-term plasticity properties of neurotransmission.

U2 - 10.1016/j.celrep.2020.107960

DO - 10.1016/j.celrep.2020.107960

M3 - SCORING: Journal article

VL - 32

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 5

M1 - 107960

ER -