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A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions

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A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions. / Kari, Vijayalakshmi; Karpiuk, Oleksandra; Tieg, Bettina; Kriegs, Malte; Dikomey, Ekkehard; Krebber, Heike; Begus-Nahrmann, Yvonne; Johnsen, Steven A.

In: PLOS ONE, Vol. 8, No. 5, 01.01.2013, p. e63745.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kari, V, Karpiuk, O, Tieg, B, Kriegs, M, Dikomey, E, Krebber, H, Begus-Nahrmann, Y & Johnsen, SA 2013, 'A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions', PLOS ONE, vol. 8, no. 5, pp. e63745. https://doi.org/10.1371/journal.pone.0063745

APA

Kari, V., Karpiuk, O., Tieg, B., Kriegs, M., Dikomey, E., Krebber, H., Begus-Nahrmann, Y., & Johnsen, S. A. (2013). A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions. PLOS ONE, 8(5), e63745. https://doi.org/10.1371/journal.pone.0063745

Vancouver

Kari V, Karpiuk O, Tieg B, Kriegs M, Dikomey E, Krebber H et al. A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions. PLOS ONE. 2013 Jan 1;8(5):e63745. https://doi.org/10.1371/journal.pone.0063745

Bibtex

@article{84f6fe8b57bb41ceb1f58b6754243fbc,
title = "A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions",
abstract = "Unlike other metazoan mRNAs, replication-dependent histone gene transcripts are not polyadenylated but instead have a conserved stem-loop structure at their 3' end. Our previous work has shown that under certain conditions replication-dependent histone genes can produce alternative transcripts that are polyadenylated at the 3' end and, in some cases, spliced. A number of microarray studies examining the expression of polyadenylated mRNAs identified changes in the levels of histone transcripts e.g. during differentiation and tumorigenesis. However, it remains unknown which histone genes produce polyadenylated transcripts and which conditions regulate this process. In the present study we examined the expression and polyadenylation of the human histone H2B gene complement in various cell lines. We demonstrate that H2B genes display a distinct expression pattern that is varies between different cell lines. Further we show that the fraction of polyadenylated HIST1H2BD and HIST1H2AC transcripts is increased during differentiation of human mesenchymal stem cells (hMSCs) and human fetal osteoblast (hFOB 1.19). Furthermore, we observed an increased fraction of polyadenylated transcripts produced from the histone genes in cells following ionizing radiation. Finally, we show that polyadenylated transcripts are transported to the cytoplasm and found on polyribosomes. Thus, we propose that the production of polyadenylated histone mRNAs from replication-dependent histone genes is a regulated process induced under specific cellular circumstances.",
keywords = "Cell Cycle Checkpoints, Cell Differentiation, Cell Line, Tumor, Cytoplasm, DNA Replication, Gene Expression, HCT116 Cells, Histones, Humans, Mesenchymal Stromal Cells, Osteoblasts, Polyribosomes, RNA, Messenger, Transcription, Genetic, Tumor Suppressor Protein p53, Up-Regulation",
author = "Vijayalakshmi Kari and Oleksandra Karpiuk and Bettina Tieg and Malte Kriegs and Ekkehard Dikomey and Heike Krebber and Yvonne Begus-Nahrmann and Johnsen, {Steven A}",
year = "2013",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0063745",
language = "English",
volume = "8",
pages = "e63745",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

RIS

TY - JOUR

T1 - A subset of histone H2B genes produces polyadenylated mRNAs under a variety of cellular conditions

AU - Kari, Vijayalakshmi

AU - Karpiuk, Oleksandra

AU - Tieg, Bettina

AU - Kriegs, Malte

AU - Dikomey, Ekkehard

AU - Krebber, Heike

AU - Begus-Nahrmann, Yvonne

AU - Johnsen, Steven A

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Unlike other metazoan mRNAs, replication-dependent histone gene transcripts are not polyadenylated but instead have a conserved stem-loop structure at their 3' end. Our previous work has shown that under certain conditions replication-dependent histone genes can produce alternative transcripts that are polyadenylated at the 3' end and, in some cases, spliced. A number of microarray studies examining the expression of polyadenylated mRNAs identified changes in the levels of histone transcripts e.g. during differentiation and tumorigenesis. However, it remains unknown which histone genes produce polyadenylated transcripts and which conditions regulate this process. In the present study we examined the expression and polyadenylation of the human histone H2B gene complement in various cell lines. We demonstrate that H2B genes display a distinct expression pattern that is varies between different cell lines. Further we show that the fraction of polyadenylated HIST1H2BD and HIST1H2AC transcripts is increased during differentiation of human mesenchymal stem cells (hMSCs) and human fetal osteoblast (hFOB 1.19). Furthermore, we observed an increased fraction of polyadenylated transcripts produced from the histone genes in cells following ionizing radiation. Finally, we show that polyadenylated transcripts are transported to the cytoplasm and found on polyribosomes. Thus, we propose that the production of polyadenylated histone mRNAs from replication-dependent histone genes is a regulated process induced under specific cellular circumstances.

AB - Unlike other metazoan mRNAs, replication-dependent histone gene transcripts are not polyadenylated but instead have a conserved stem-loop structure at their 3' end. Our previous work has shown that under certain conditions replication-dependent histone genes can produce alternative transcripts that are polyadenylated at the 3' end and, in some cases, spliced. A number of microarray studies examining the expression of polyadenylated mRNAs identified changes in the levels of histone transcripts e.g. during differentiation and tumorigenesis. However, it remains unknown which histone genes produce polyadenylated transcripts and which conditions regulate this process. In the present study we examined the expression and polyadenylation of the human histone H2B gene complement in various cell lines. We demonstrate that H2B genes display a distinct expression pattern that is varies between different cell lines. Further we show that the fraction of polyadenylated HIST1H2BD and HIST1H2AC transcripts is increased during differentiation of human mesenchymal stem cells (hMSCs) and human fetal osteoblast (hFOB 1.19). Furthermore, we observed an increased fraction of polyadenylated transcripts produced from the histone genes in cells following ionizing radiation. Finally, we show that polyadenylated transcripts are transported to the cytoplasm and found on polyribosomes. Thus, we propose that the production of polyadenylated histone mRNAs from replication-dependent histone genes is a regulated process induced under specific cellular circumstances.

KW - Cell Cycle Checkpoints

KW - Cell Differentiation

KW - Cell Line, Tumor

KW - Cytoplasm

KW - DNA Replication

KW - Gene Expression

KW - HCT116 Cells

KW - Histones

KW - Humans

KW - Mesenchymal Stromal Cells

KW - Osteoblasts

KW - Polyribosomes

KW - RNA, Messenger

KW - Transcription, Genetic

KW - Tumor Suppressor Protein p53

KW - Up-Regulation

U2 - 10.1371/journal.pone.0063745

DO - 10.1371/journal.pone.0063745

M3 - SCORING: Journal article

C2 - 23717473

VL - 8

SP - e63745

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 5

ER -