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A slit-diaphragm-associated protein network for dynamic control of renal filtration

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A slit-diaphragm-associated protein network for dynamic control of renal filtration. / Kocylowski, Maciej K; Aypek, Hande; Bildl, Wolfgang; Helmstädter, Martin; Trachte, Philipp; Dumoulin, Bernhard; Wittösch, Sina; Kühne, Lukas; Aukschun, Ute; Teetzen, Carolin; Kretz, Oliver; Gaal, Botond; Kulik, Akos; Antignac, Corinne; Mollet, Geraldine; Köttgen, Anna; Göcmen, Burulca; Schwenk, Jochen; Schulte, Uwe; Huber, Tobias B; Fakler, Bernd; Grahammer, Florian.

In: NAT COMMUN, Vol. 13, No. 1, 28.10.2022, p. 6446.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kocylowski, MK, Aypek, H, Bildl, W, Helmstädter, M, Trachte, P, Dumoulin, B, Wittösch, S, Kühne, L, Aukschun, U, Teetzen, C, Kretz, O, Gaal, B, Kulik, A, Antignac, C, Mollet, G, Köttgen, A, Göcmen, B, Schwenk, J, Schulte, U, Huber, TB, Fakler, B & Grahammer, F 2022, 'A slit-diaphragm-associated protein network for dynamic control of renal filtration', NAT COMMUN, vol. 13, no. 1, pp. 6446. https://doi.org/10.1038/s41467-022-33748-1

APA

Kocylowski, M. K., Aypek, H., Bildl, W., Helmstädter, M., Trachte, P., Dumoulin, B., Wittösch, S., Kühne, L., Aukschun, U., Teetzen, C., Kretz, O., Gaal, B., Kulik, A., Antignac, C., Mollet, G., Köttgen, A., Göcmen, B., Schwenk, J., Schulte, U., ... Grahammer, F. (2022). A slit-diaphragm-associated protein network for dynamic control of renal filtration. NAT COMMUN, 13(1), 6446. https://doi.org/10.1038/s41467-022-33748-1

Vancouver

Kocylowski MK, Aypek H, Bildl W, Helmstädter M, Trachte P, Dumoulin B et al. A slit-diaphragm-associated protein network for dynamic control of renal filtration. NAT COMMUN. 2022 Oct 28;13(1):6446. https://doi.org/10.1038/s41467-022-33748-1

Bibtex

@article{01a95103f2774f21bb743bf7f0e4f707,
title = "A slit-diaphragm-associated protein network for dynamic control of renal filtration",
abstract = "The filtration of blood in the kidney which is crucial for mammalian life is determined by the slit-diaphragm, a cell-cell junction between the foot processes of renal podocytes. The slit-diaphragm is thought to operate as final barrier or as molecular sensor of renal filtration. Using high-resolution proteomic analysis of slit-diaphragms affinity-isolated from rodent kidney, we show that the native slit-diaphragm is built from the junction-forming components Nephrin, Neph1 and Podocin and a co-assembled high-molecular weight network of proteins. The network constituents cover distinct classes of proteins including signaling-receptors, kinases/phosphatases, transporters and scaffolds. Knockout or knock-down of either the core components or the selected network constituents tyrosine kinase MER (MERTK), atrial natriuretic peptide-receptor C (ANPRC), integral membrane protein 2B (ITM2B), membrane-associated guanylate-kinase, WW and PDZ-domain-containing protein1 (MAGI1) and amyloid protein A4 resulted in target-specific impairment or disruption of the filtration process. Our results identify the slit-diaphragm as a multi-component system that is endowed with context-dependent dynamics via a co-assembled protein network.",
keywords = "Animals, Diaphragm, Proteomics, Podocytes/metabolism, Kidney Glomerulus, Intercellular Junctions, Mammals",
author = "Kocylowski, {Maciej K} and Hande Aypek and Wolfgang Bildl and Martin Helmst{\"a}dter and Philipp Trachte and Bernhard Dumoulin and Sina Witt{\"o}sch and Lukas K{\"u}hne and Ute Aukschun and Carolin Teetzen and Oliver Kretz and Botond Gaal and Akos Kulik and Corinne Antignac and Geraldine Mollet and Anna K{\"o}ttgen and Burulca G{\"o}cmen and Jochen Schwenk and Uwe Schulte and Huber, {Tobias B} and Bernd Fakler and Florian Grahammer",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
month = oct,
day = "28",
doi = "10.1038/s41467-022-33748-1",
language = "English",
volume = "13",
pages = "6446",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - A slit-diaphragm-associated protein network for dynamic control of renal filtration

AU - Kocylowski, Maciej K

AU - Aypek, Hande

AU - Bildl, Wolfgang

AU - Helmstädter, Martin

AU - Trachte, Philipp

AU - Dumoulin, Bernhard

AU - Wittösch, Sina

AU - Kühne, Lukas

AU - Aukschun, Ute

AU - Teetzen, Carolin

AU - Kretz, Oliver

AU - Gaal, Botond

AU - Kulik, Akos

AU - Antignac, Corinne

AU - Mollet, Geraldine

AU - Köttgen, Anna

AU - Göcmen, Burulca

AU - Schwenk, Jochen

AU - Schulte, Uwe

AU - Huber, Tobias B

AU - Fakler, Bernd

AU - Grahammer, Florian

N1 - © 2022. The Author(s).

PY - 2022/10/28

Y1 - 2022/10/28

N2 - The filtration of blood in the kidney which is crucial for mammalian life is determined by the slit-diaphragm, a cell-cell junction between the foot processes of renal podocytes. The slit-diaphragm is thought to operate as final barrier or as molecular sensor of renal filtration. Using high-resolution proteomic analysis of slit-diaphragms affinity-isolated from rodent kidney, we show that the native slit-diaphragm is built from the junction-forming components Nephrin, Neph1 and Podocin and a co-assembled high-molecular weight network of proteins. The network constituents cover distinct classes of proteins including signaling-receptors, kinases/phosphatases, transporters and scaffolds. Knockout or knock-down of either the core components or the selected network constituents tyrosine kinase MER (MERTK), atrial natriuretic peptide-receptor C (ANPRC), integral membrane protein 2B (ITM2B), membrane-associated guanylate-kinase, WW and PDZ-domain-containing protein1 (MAGI1) and amyloid protein A4 resulted in target-specific impairment or disruption of the filtration process. Our results identify the slit-diaphragm as a multi-component system that is endowed with context-dependent dynamics via a co-assembled protein network.

AB - The filtration of blood in the kidney which is crucial for mammalian life is determined by the slit-diaphragm, a cell-cell junction between the foot processes of renal podocytes. The slit-diaphragm is thought to operate as final barrier or as molecular sensor of renal filtration. Using high-resolution proteomic analysis of slit-diaphragms affinity-isolated from rodent kidney, we show that the native slit-diaphragm is built from the junction-forming components Nephrin, Neph1 and Podocin and a co-assembled high-molecular weight network of proteins. The network constituents cover distinct classes of proteins including signaling-receptors, kinases/phosphatases, transporters and scaffolds. Knockout or knock-down of either the core components or the selected network constituents tyrosine kinase MER (MERTK), atrial natriuretic peptide-receptor C (ANPRC), integral membrane protein 2B (ITM2B), membrane-associated guanylate-kinase, WW and PDZ-domain-containing protein1 (MAGI1) and amyloid protein A4 resulted in target-specific impairment or disruption of the filtration process. Our results identify the slit-diaphragm as a multi-component system that is endowed with context-dependent dynamics via a co-assembled protein network.

KW - Animals

KW - Diaphragm

KW - Proteomics

KW - Podocytes/metabolism

KW - Kidney Glomerulus

KW - Intercellular Junctions

KW - Mammals

U2 - 10.1038/s41467-022-33748-1

DO - 10.1038/s41467-022-33748-1

M3 - SCORING: Journal article

C2 - 36307401

VL - 13

SP - 6446

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -