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A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction

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A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction. / Kiene, P; Milde-Langosch, K; Runkel, M; Schulz, K; Löning, T.

In: Virchows Arch A Pathol Anat Histopathol, Vol. 420, No. 3, 1992, p. 269-73.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kiene, P, Milde-Langosch, K, Runkel, M, Schulz, K & Löning, T 1992, 'A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction', Virchows Arch A Pathol Anat Histopathol, vol. 420, no. 3, pp. 269-73.

APA

Kiene, P., Milde-Langosch, K., Runkel, M., Schulz, K., & Löning, T. (1992). A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction. Virchows Arch A Pathol Anat Histopathol, 420(3), 269-73.

Vancouver

Kiene P, Milde-Langosch K, Runkel M, Schulz K, Löning T. A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction. Virchows Arch A Pathol Anat Histopathol. 1992;420(3):269-73.

Bibtex

@article{bbeb594045c24afa8efd27363322efb2,
title = "A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction",
abstract = "The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.",
keywords = "Acquired Immunodeficiency Syndrome, Autopsy, Cytomegalovirus, DNA, Viral, Female, Heart, Humans, Kidney, Lung, Myocardium, Nasopharyngeal Neoplasms, Papillomaviridae, Placenta, Polymerase Chain Reaction, Pregnancy, Vulvar Neoplasms",
author = "P Kiene and K Milde-Langosch and M Runkel and K Schulz and T L{\"o}ning",
year = "1992",
language = "English",
volume = "420",
pages = "269--73",
journal = "VIRCHOWS ARCH",
issn = "0945-6317",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction

AU - Kiene, P

AU - Milde-Langosch, K

AU - Runkel, M

AU - Schulz, K

AU - Löning, T

PY - 1992

Y1 - 1992

N2 - The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.

AB - The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.

KW - Acquired Immunodeficiency Syndrome

KW - Autopsy

KW - Cytomegalovirus

KW - DNA, Viral

KW - Female

KW - Heart

KW - Humans

KW - Kidney

KW - Lung

KW - Myocardium

KW - Nasopharyngeal Neoplasms

KW - Papillomaviridae

KW - Placenta

KW - Polymerase Chain Reaction

KW - Pregnancy

KW - Vulvar Neoplasms

M3 - SCORING: Journal article

C2 - 1313196

VL - 420

SP - 269

EP - 273

JO - VIRCHOWS ARCH

JF - VIRCHOWS ARCH

SN - 0945-6317

IS - 3

ER -