A prospective time-course study on serological testing for human immunodeficiency virus, hepatitis B virus and hepatitis C virus with blood samples taken up to 48 h after death.
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A prospective time-course study on serological testing for human immunodeficiency virus, hepatitis B virus and hepatitis C virus with blood samples taken up to 48 h after death. / Edler, Carolin; Wulff, Birgit; Schröder, Ann Sophie; Wilkemeyer, Ina; Polywka, Susanne; Meyer, Thomas; Kalus, Ulrich; Pruss, Axel.
In: J MED MICROBIOL, Vol. 60, No. Pt 7, Pt 7, 2011, p. 920-926.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - A prospective time-course study on serological testing for human immunodeficiency virus, hepatitis B virus and hepatitis C virus with blood samples taken up to 48 h after death.
AU - Edler, Carolin
AU - Wulff, Birgit
AU - Schröder, Ann Sophie
AU - Wilkemeyer, Ina
AU - Polywka, Susanne
AU - Meyer, Thomas
AU - Kalus, Ulrich
AU - Pruss, Axel
PY - 2011
Y1 - 2011
N2 - The transmission of viral and non-viral infectious pathogens continues to be the most serious of the potential adverse effects of allogenic tissue transplantations. EU Directive 2006/17/EC stipulates that cadaveric blood specimens for serology testing in the context of post-mortem tissue donation must be taken not later than 24 h post-mortem. An expanded time slot would significantly improve the availability of tissue donations, but there are no significant data on the stability of infectious serology assays for anti-human immunodeficiency virus (HIV), anti-hepatitis C virus (HCV), hepatitis B virus (HBV) surface antigen (HBsAg) and anti-HBC core antigen (HBc) in samples collected more than 24 h post-mortem. In this prospective study, serum samples of 30 deceased persons were taken upon admission to the Institute of Forensic Medicine (University Hospital Hamburg-Eppendorf, Germany) and at 12, 24, 36 and 48 h post-mortem. All samples were measured twice, first using the Abbott AxSYM system, and then after ~9 months of storage at -70 °C using the BEP III System with Siemens and Ortho reagents. For HIV, six deceased persons with a pre-mortem HIV history were included. All samples (at committal and at 12, 24, 36, 48 h) were reactive. Indeterminate or false-negative results did not occur. For HCV, 17 deceased persons with a pre-mortem HCV history were included; 16 samples were reactive up to 48 h and one was reactive at 36 h post-mortem (48 h sample was not available). Indeterminate or false-negative results did not occur. For HBV, nine deceased persons were included: five samples were initially positive for HBsAg and remained positive up to 48 h, and eight of the samples were reactive for anti-HBc up to 48 h and one up to 36 h post-mortem (48 h sample was not available). Indeterminate or false-negative results did not occur. These data suggest that infectious serological testing may be extended for blood samples of potential tissue donors collected up to 48 h post-mortem to detect antibodies or antigens for HIV, HBV and HCV.
AB - The transmission of viral and non-viral infectious pathogens continues to be the most serious of the potential adverse effects of allogenic tissue transplantations. EU Directive 2006/17/EC stipulates that cadaveric blood specimens for serology testing in the context of post-mortem tissue donation must be taken not later than 24 h post-mortem. An expanded time slot would significantly improve the availability of tissue donations, but there are no significant data on the stability of infectious serology assays for anti-human immunodeficiency virus (HIV), anti-hepatitis C virus (HCV), hepatitis B virus (HBV) surface antigen (HBsAg) and anti-HBC core antigen (HBc) in samples collected more than 24 h post-mortem. In this prospective study, serum samples of 30 deceased persons were taken upon admission to the Institute of Forensic Medicine (University Hospital Hamburg-Eppendorf, Germany) and at 12, 24, 36 and 48 h post-mortem. All samples were measured twice, first using the Abbott AxSYM system, and then after ~9 months of storage at -70 °C using the BEP III System with Siemens and Ortho reagents. For HIV, six deceased persons with a pre-mortem HIV history were included. All samples (at committal and at 12, 24, 36, 48 h) were reactive. Indeterminate or false-negative results did not occur. For HCV, 17 deceased persons with a pre-mortem HCV history were included; 16 samples were reactive up to 48 h and one was reactive at 36 h post-mortem (48 h sample was not available). Indeterminate or false-negative results did not occur. For HBV, nine deceased persons were included: five samples were initially positive for HBsAg and remained positive up to 48 h, and eight of the samples were reactive for anti-HBc up to 48 h and one up to 36 h post-mortem (48 h sample was not available). Indeterminate or false-negative results did not occur. These data suggest that infectious serological testing may be extended for blood samples of potential tissue donors collected up to 48 h post-mortem to detect antibodies or antigens for HIV, HBV and HCV.
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Prospective Studies
KW - Time Factors
KW - Antibodies, Viral/blood
KW - Antigens, Viral/blood
KW - HIV Infections/blood/diagnosis
KW - HIV-1/immunology/isolation & purification
KW - Hepacivirus/immunology/isolation & purification
KW - Hepatitis B/blood/diagnosis
KW - Hepatitis B virus/immunology/isolation & purification
KW - Hepatitis C/blood/diagnosis
KW - Serologic Tests/methods
KW - Adult
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Prospective Studies
KW - Time Factors
KW - Antibodies, Viral/blood
KW - Antigens, Viral/blood
KW - HIV Infections/blood/diagnosis
KW - HIV-1/immunology/isolation & purification
KW - Hepacivirus/immunology/isolation & purification
KW - Hepatitis B/blood/diagnosis
KW - Hepatitis B virus/immunology/isolation & purification
KW - Hepatitis C/blood/diagnosis
KW - Serologic Tests/methods
M3 - SCORING: Journal article
VL - 60
SP - 920
EP - 926
JO - J MED MICROBIOL
JF - J MED MICROBIOL
SN - 0022-2615
IS - Pt 7
M1 - Pt 7
ER -