A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development.
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A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development. / Methner, A; Hermey, Guido; Schinke, B; Hermans-Borgmeyer, I.
In: BIOCHEM BIOPH RES CO, Vol. 233, No. 2, 2, 1997, p. 336-342.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A novel G protein-coupled receptor with homology to neuropeptide and chemoattractant receptors expressed during bone development.
AU - Methner, A
AU - Hermey, Guido
AU - Schinke, B
AU - Hermans-Borgmeyer, I
PY - 1997
Y1 - 1997
N2 - A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism.
AB - A novel G protein-coupled receptor was isolated from a cDNA derived from the cell line NH15-CA2 and a cDNA library from adult mouse brain using a PCR cloning strategy. The amino acid sequence of the candidate receptor DEZ showed homology to neuropeptide and chemoattractant receptors. Highest overall homology was found with the orphan receptor GPR-1 (65%), the angiotensin II receptor (62%), and the C5a anaphylatoxin receptor (60%). Northern blot analysis of dez revealed a predominant 2.6 kb mRNA species in NH15-CA2 cells. In situ hybridization experiments showed that dez is differentially regulated during development, with a prominent expression in developing osseous and cartilaginous tissue. It was also detectable in the adult parathyroid glands, hinting at a possible function in bone metabolism.
M3 - SCORING: Zeitschriftenaufsatz
VL - 233
SP - 336
EP - 342
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 2
M1 - 2
ER -
