A novel DFNB1 deletion allele supports the existence of a distant cis-regulatory region that controls GJB2 and GJB6 expression.
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A novel DFNB1 deletion allele supports the existence of a distant cis-regulatory region that controls GJB2 and GJB6 expression. / Wilch, E; Azaiez, H; Fisher, R A; Elfenbein, J; Murgia, A; Birkenhäger, R; Bolz, H; Da Silva-Costa, S M; Del Castillo, I; Haaf, T; Hoefsloot, L; Kremer, H; Kubisch, Christian; Le Marechal, C; Pandya, A; Sartorato, E L; Schneider, E; Van Camp, G; Wuyts, W; Smith, R J H; Friderici, K H.
In: CLIN GENET, Vol. 78, No. 3, 3, 2010, p. 267-274.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - A novel DFNB1 deletion allele supports the existence of a distant cis-regulatory region that controls GJB2 and GJB6 expression.
AU - Wilch, E
AU - Azaiez, H
AU - Fisher, R A
AU - Elfenbein, J
AU - Murgia, A
AU - Birkenhäger, R
AU - Bolz, H
AU - Da Silva-Costa, S M
AU - Del Castillo, I
AU - Haaf, T
AU - Hoefsloot, L
AU - Kremer, H
AU - Kubisch, Christian
AU - Le Marechal, C
AU - Pandya, A
AU - Sartorato, E L
AU - Schneider, E
AU - Van Camp, G
AU - Wuyts, W
AU - Smith, R J H
AU - Friderici, K H
PY - 2010
Y1 - 2010
N2 - Eleven affected members of a large German-American family segregating recessively inherited, congenital, non-syndromic sensorineural hearing loss (SNHL) were found to be homozygous for the common 35delG mutation of GJB2, the gene encoding the gap junction protein Connexin 26. Surprisingly, four additional family members with bilateral profound SNHL carried only a single 35delG mutation. Previously, we demonstrated reduced expression of both GJB2 and GJB6 mRNA from the allele carried in trans with that bearing the 35delG mutation in these four persons. Using array comparative genome hybridization (array CGH), we have now identified on this allele a deletion of 131.4 kb whose proximal breakpoint lies more than 100 kb upstream of the transcriptional start sites of GJB2 and GJB6. This deletion, del(chr13:19,837,344-19,968,698), segregates as a completely penetrant DFNB1 allele in this family. It is not present in 528 persons with SNHL and monoallelic mutation of GJB2 or GJB6, and we have not identified any other candidate pathogenic copy number variation by arrayCGH in a subset of 10 such persons. Characterization of distant GJB2/GJB6 cis-regulatory regions evidenced by this allele may be required to find the 'missing' DFNB1 mutations that are believed to exist.
AB - Eleven affected members of a large German-American family segregating recessively inherited, congenital, non-syndromic sensorineural hearing loss (SNHL) were found to be homozygous for the common 35delG mutation of GJB2, the gene encoding the gap junction protein Connexin 26. Surprisingly, four additional family members with bilateral profound SNHL carried only a single 35delG mutation. Previously, we demonstrated reduced expression of both GJB2 and GJB6 mRNA from the allele carried in trans with that bearing the 35delG mutation in these four persons. Using array comparative genome hybridization (array CGH), we have now identified on this allele a deletion of 131.4 kb whose proximal breakpoint lies more than 100 kb upstream of the transcriptional start sites of GJB2 and GJB6. This deletion, del(chr13:19,837,344-19,968,698), segregates as a completely penetrant DFNB1 allele in this family. It is not present in 528 persons with SNHL and monoallelic mutation of GJB2 or GJB6, and we have not identified any other candidate pathogenic copy number variation by arrayCGH in a subset of 10 such persons. Characterization of distant GJB2/GJB6 cis-regulatory regions evidenced by this allele may be required to find the 'missing' DFNB1 mutations that are believed to exist.
KW - Humans
KW - Male
KW - Female
KW - Genotype
KW - Molecular Sequence Data
KW - Base Sequence
KW - Comparative Genomic Hybridization
KW - Pedigree
KW - Family Health
KW - Alleles
KW - Genetic Testing
KW - Chromosome Deletion
KW - Gene Expression Regulation
KW - Sequence Deletion
KW - Sequence Homology, Nucleic Acid
KW - Chromosomes, Human, Pair 13/genetics
KW - Connexins/genetics
KW - Hearing Loss, Sensorineural/diagnosis/genetics
KW - Penetrance
KW - Regulatory Sequences, Nucleic Acid/genetics
KW - Humans
KW - Male
KW - Female
KW - Genotype
KW - Molecular Sequence Data
KW - Base Sequence
KW - Comparative Genomic Hybridization
KW - Pedigree
KW - Family Health
KW - Alleles
KW - Genetic Testing
KW - Chromosome Deletion
KW - Gene Expression Regulation
KW - Sequence Deletion
KW - Sequence Homology, Nucleic Acid
KW - Chromosomes, Human, Pair 13/genetics
KW - Connexins/genetics
KW - Hearing Loss, Sensorineural/diagnosis/genetics
KW - Penetrance
KW - Regulatory Sequences, Nucleic Acid/genetics
M3 - SCORING: Journal article
VL - 78
SP - 267
EP - 274
JO - CLIN GENET
JF - CLIN GENET
SN - 0009-9163
IS - 3
M1 - 3
ER -