A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI)
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A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI). / Salzbrunn, A; Benson, D M; Holstein, A F; Schulze, W.
In: HUM REPROD, Vol. 11, No. 4, 04.1996, p. 752-5.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A new concept for the extraction of testicular spermatozoa as a tool for assisted fertilization (ICSI)
AU - Salzbrunn, A
AU - Benson, D M
AU - Holstein, A F
AU - Schulze, W
PY - 1996/4
Y1 - 1996/4
N2 - A new method is described for the enzymatic preparation of testicular tissue to obtain vital spermatozoa for ICSI. The tissue obtained from both testes by biopsy is studied by means of the semi-thin section method as well as being cryo-preserved. If spermatid formation is assured by semi-thin section histology, vital spermatozoa from the cryo-preserved portion of testicular tissue are enzymatically prepared. In this way it is possible to optimize the reproductive medical treatment for those couples in whom an extracorporal fertilization of oocytes by means of testicular spermatozoa is being considered.
AB - A new method is described for the enzymatic preparation of testicular tissue to obtain vital spermatozoa for ICSI. The tissue obtained from both testes by biopsy is studied by means of the semi-thin section method as well as being cryo-preserved. If spermatid formation is assured by semi-thin section histology, vital spermatozoa from the cryo-preserved portion of testicular tissue are enzymatically prepared. In this way it is possible to optimize the reproductive medical treatment for those couples in whom an extracorporal fertilization of oocytes by means of testicular spermatozoa is being considered.
KW - Aged
KW - Cell Separation
KW - Fertilization in Vitro
KW - Humans
KW - Male
KW - Spermatozoa
KW - Testis
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 8671322
VL - 11
SP - 752
EP - 755
JO - HUM REPROD
JF - HUM REPROD
SN - 0268-1161
IS - 4
ER -