A new approach to demonstrate cellular activity in bone formation adjacent to implants
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A new approach to demonstrate cellular activity in bone formation adjacent to implants. / Röser, K; Johansson, C B; Donath, K; Albrektsson, T.
In: J Biomed Mater Res, Vol. 51, No. 2, 08.2000, p. 280-91.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A new approach to demonstrate cellular activity in bone formation adjacent to implants
AU - Röser, K
AU - Johansson, C B
AU - Donath, K
AU - Albrektsson, T
N1 - Copyright 2000 John Wiley & Sons, Inc.
PY - 2000/8
Y1 - 2000/8
N2 - Bone tissue repeatedly formed in titanium 6-aluminum 4-vanadium rabbit bone harvest implants was collected in vivo at various times between 12 days and 5 weeks. Qualitative and quantitative examinations on undecalcified thin sections were performed in the light microscope. The amount of bone tissue was calculated on routinely stained sections. Alkaline (ALP) and acid phosphatase (ACP) enzyme activities were investigated. We also performed immunohistological detection of bone matrix proteins. Increasing bone density as well as an increasing mineralization of the tissue was observed in the biopsies with increasing time. The ALP and ACP activities were similar at short times (12 days and 2 weeks). With increasing time the ALP activity was stronger than that of ACP. The results from the immunohistochemical detection of osteonectin, osteopontin, bone sialoprotein, and collagen I and II demonstrated changes in the tissue differentiation with time. The tissue formation in the canal became more mature with time of ingrowth, as observed with the various techniques used in this study. Owing to these methodical developments, undecalcified ground sections may be used for detailed analysis of various phases of tissue formation in close proximity to implants.
AB - Bone tissue repeatedly formed in titanium 6-aluminum 4-vanadium rabbit bone harvest implants was collected in vivo at various times between 12 days and 5 weeks. Qualitative and quantitative examinations on undecalcified thin sections were performed in the light microscope. The amount of bone tissue was calculated on routinely stained sections. Alkaline (ALP) and acid phosphatase (ACP) enzyme activities were investigated. We also performed immunohistological detection of bone matrix proteins. Increasing bone density as well as an increasing mineralization of the tissue was observed in the biopsies with increasing time. The ALP and ACP activities were similar at short times (12 days and 2 weeks). With increasing time the ALP activity was stronger than that of ACP. The results from the immunohistochemical detection of osteonectin, osteopontin, bone sialoprotein, and collagen I and II demonstrated changes in the tissue differentiation with time. The tissue formation in the canal became more mature with time of ingrowth, as observed with the various techniques used in this study. Owing to these methodical developments, undecalcified ground sections may be used for detailed analysis of various phases of tissue formation in close proximity to implants.
KW - Acid Phosphatase
KW - Alkaline Phosphatase
KW - Animals
KW - Biocompatible Materials
KW - Biomarkers
KW - Bone Screws
KW - Collagen
KW - Integrin-Binding Sialoprotein
KW - Internal Fixators
KW - Osteogenesis
KW - Osteopontin
KW - Phosphoproteins
KW - Rabbits
KW - Sialoglycoproteins
KW - Titanium
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 10825228
VL - 51
SP - 280
EP - 291
IS - 2
ER -
