A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.

Hans-Peter Beck-Bornholdt

A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.

Standard

A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations. / Beck-Bornholdt, Hans-Peter.

In: Cell Tissue Kinet, Vol. 11, No. 2, 2, 1978, p. 139-148.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Beck-Bornholdt, H-P 1978, 'A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.', Cell Tissue Kinet, vol. 11, no. 2, 2, pp. 139-148.

APA

Beck-Bornholdt, H-P. (1978). A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations. Cell Tissue Kinet, 11(2), 139-148. [2].

Vancouver

Beck-Bornholdt H-P. A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations. Cell Tissue Kinet. 1978;11(2):139-148. 2.

Bibtex

@article{230b80afd34e405082590146caeb6995,

title = "A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.",

abstract = "L-cells synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G1, S, G2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S-phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.",

author = "Hans-Peter Beck-Bornholdt",

year = "1978",

language = "Deutsch",

volume = "11",

pages = "139--148",

number = "2",

}

RIS

TY - JOUR

T1 - A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.

AU - Beck-Bornholdt, Hans-Peter

PY - 1978

Y1 - 1978

N2 - L-cells synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G1, S, G2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S-phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.

AB - L-cells synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G1, S, G2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S-phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.

M3 - SCORING: Zeitschriftenaufsatz

VL - 11

SP - 139

EP - 148

IS - 2

M1 - 2

ER -