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A mouse cdc25 homolog is differentially and developmentally expressed

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A mouse cdc25 homolog is differentially and developmentally expressed. / Kakizuka, A; Sebastian, B; Borgmeyer, U; Hermans-Borgmeyer, I; Bolado, J; Hunter, T; Hoekstra, M F; Evans, R M.

In: GENE DEV, Vol. 6, No. 4, 04.1992, p. 578-90.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kakizuka, A, Sebastian, B, Borgmeyer, U, Hermans-Borgmeyer, I, Bolado, J, Hunter, T, Hoekstra, MF & Evans, RM 1992, 'A mouse cdc25 homolog is differentially and developmentally expressed', GENE DEV, vol. 6, no. 4, pp. 578-90.

APA

Kakizuka, A., Sebastian, B., Borgmeyer, U., Hermans-Borgmeyer, I., Bolado, J., Hunter, T., Hoekstra, M. F., & Evans, R. M. (1992). A mouse cdc25 homolog is differentially and developmentally expressed. GENE DEV, 6(4), 578-90.

Vancouver

Kakizuka A, Sebastian B, Borgmeyer U, Hermans-Borgmeyer I, Bolado J, Hunter T et al. A mouse cdc25 homolog is differentially and developmentally expressed. GENE DEV. 1992 Apr;6(4):578-90.

Bibtex

@article{aca852e2f46342f4b6140d71f16650b8,
title = "A mouse cdc25 homolog is differentially and developmentally expressed",
abstract = "The timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. For fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. We report the identification and characterization of a structural and functional mouse homolog, Cdc25M2, of the cdc25 phosphatase. Cdc25M2 shows high sequence identity to the previously reported human homolog cdc25Hu2. Cdc25M2 can functionally complement for a Schizosaccharomyces pombe cdc25ts mutation, and when expressed in Escherichia coli and purified, Cdc25M2 is an active phosphatase. cdc25M2 mRNA shows variation in expression in different tissues in the mouse embryo and is expressed in a developmental and cell-cycle-dependent fashion. We suggest that the expression and accumulation of the cdc25 mitotic inducer may play a critical role in the regulation of mouse development.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, CDC2 Protein Kinase, DNA, Fungal, Gene Expression, Genes, Fungal, Mice, Mitosis, Molecular Sequence Data, Mutation, Nervous System, Phosphoric Monoester Hydrolases, Phosphorylation, Polymerase Chain Reaction, Proteins, RNA, Messenger, Schizosaccharomyces, Sequence Alignment, Sequence Homology, Nucleic Acid, Thymidine, cdc25 Phosphatases",
author = "A Kakizuka and B Sebastian and U Borgmeyer and I Hermans-Borgmeyer and J Bolado and T Hunter and Hoekstra, {M F} and Evans, {R M}",
year = "1992",
month = apr,
language = "English",
volume = "6",
pages = "578--90",
journal = "GENE DEV",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "4",

}

RIS

TY - JOUR

T1 - A mouse cdc25 homolog is differentially and developmentally expressed

AU - Kakizuka, A

AU - Sebastian, B

AU - Borgmeyer, U

AU - Hermans-Borgmeyer, I

AU - Bolado, J

AU - Hunter, T

AU - Hoekstra, M F

AU - Evans, R M

PY - 1992/4

Y1 - 1992/4

N2 - The timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. For fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. We report the identification and characterization of a structural and functional mouse homolog, Cdc25M2, of the cdc25 phosphatase. Cdc25M2 shows high sequence identity to the previously reported human homolog cdc25Hu2. Cdc25M2 can functionally complement for a Schizosaccharomyces pombe cdc25ts mutation, and when expressed in Escherichia coli and purified, Cdc25M2 is an active phosphatase. cdc25M2 mRNA shows variation in expression in different tissues in the mouse embryo and is expressed in a developmental and cell-cycle-dependent fashion. We suggest that the expression and accumulation of the cdc25 mitotic inducer may play a critical role in the regulation of mouse development.

AB - The timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. For fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. We report the identification and characterization of a structural and functional mouse homolog, Cdc25M2, of the cdc25 phosphatase. Cdc25M2 shows high sequence identity to the previously reported human homolog cdc25Hu2. Cdc25M2 can functionally complement for a Schizosaccharomyces pombe cdc25ts mutation, and when expressed in Escherichia coli and purified, Cdc25M2 is an active phosphatase. cdc25M2 mRNA shows variation in expression in different tissues in the mouse embryo and is expressed in a developmental and cell-cycle-dependent fashion. We suggest that the expression and accumulation of the cdc25 mitotic inducer may play a critical role in the regulation of mouse development.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - CDC2 Protein Kinase

KW - DNA, Fungal

KW - Gene Expression

KW - Genes, Fungal

KW - Mice

KW - Mitosis

KW - Molecular Sequence Data

KW - Mutation

KW - Nervous System

KW - Phosphoric Monoester Hydrolases

KW - Phosphorylation

KW - Polymerase Chain Reaction

KW - Proteins

KW - RNA, Messenger

KW - Schizosaccharomyces

KW - Sequence Alignment

KW - Sequence Homology, Nucleic Acid

KW - Thymidine

KW - cdc25 Phosphatases

M3 - SCORING: Journal article

C2 - 1313771

VL - 6

SP - 578

EP - 590

JO - GENE DEV

JF - GENE DEV

SN - 0890-9369

IS - 4

ER -