A major messenger ribonucleic acid of the rodent epididymis encodes a small glycosylphosphatidylinositol-anchored lymphocyte surface antigen
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A major messenger ribonucleic acid of the rodent epididymis encodes a small glycosylphosphatidylinositol-anchored lymphocyte surface antigen. / Kirchhoff, C.
In: BIOL REPROD, Vol. 50, No. 4, 04.1994, p. 896-902.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A major messenger ribonucleic acid of the rodent epididymis encodes a small glycosylphosphatidylinositol-anchored lymphocyte surface antigen
AU - Kirchhoff, C
PY - 1994/4
Y1 - 1994/4
N2 - The adult rodent epididymis and seminal vesicles were shown by polymerase chain reaction (PCR) cloning. Northern blot analysis, and in situ hybridization to be major expression sites of an mRNA encoding a small glycosylphosphatidylinositol (GPI)-anchored cell surface antigen that has been previously described as the mouse lymphocyte differentiation antigen B7, herein referred to as MB7. Sequence comparison of the corresponding epididymal cDNAs from the mouse (MB7) and the rat (RB7) revealed an unexpected divergence in the peptide coding regions of these homologous genes. A possible functional relationship to the human sperm surface and lymphocyte differentiation antigen CDw52/HE5 is discussed with respect to the highly variable mature peptide coding regions.
AB - The adult rodent epididymis and seminal vesicles were shown by polymerase chain reaction (PCR) cloning. Northern blot analysis, and in situ hybridization to be major expression sites of an mRNA encoding a small glycosylphosphatidylinositol (GPI)-anchored cell surface antigen that has been previously described as the mouse lymphocyte differentiation antigen B7, herein referred to as MB7. Sequence comparison of the corresponding epididymal cDNAs from the mouse (MB7) and the rat (RB7) revealed an unexpected divergence in the peptide coding regions of these homologous genes. A possible functional relationship to the human sperm surface and lymphocyte differentiation antigen CDw52/HE5 is discussed with respect to the highly variable mature peptide coding regions.
KW - Amino Acid Sequence
KW - Animals
KW - Antigens, CD80
KW - Base Sequence
KW - Blotting, Northern
KW - Cloning, Molecular
KW - DNA, Complementary
KW - Epididymis
KW - Glycosylphosphatidylinositols
KW - In Situ Hybridization
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Molecular Sequence Data
KW - Polymerase Chain Reaction
KW - RNA, Messenger
KW - Rats
KW - Rats, Wistar
KW - Sequence Homology
M3 - SCORING: Journal article
C2 - 7515288
VL - 50
SP - 896
EP - 902
JO - BIOL REPROD
JF - BIOL REPROD
SN - 0006-3363
IS - 4
ER -