A luminescence Western blot with enhanced sensitivity for antibodies to human immunodeficiency virus

A luminescence assay was adapted for detection of peroxidase-labelled secondary antibodies, which react specifically with antibodies to human immunodeficiency virus on Western blots. This method is about one hundred times more sensitive than either commercial enzyme-linked immunosorbent assay or the chromogenic peroxidase assay on Western blots and detects seroconversion earlier than any other technique currently available.