A Lactobacillus rhamnosus strain induces a heme oxygenase dependent increase in Foxp3+ regulatory T cells.
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A Lactobacillus rhamnosus strain induces a heme oxygenase dependent increase in Foxp3+ regulatory T cells. / Karimi, Khalil; Kandiah, Nalaayini; Chau, Jessie; Bienenstock, John; Forsythe, Paul.
In: PLOS ONE, Vol. 7, No. 10, 10, 2012, p. 47556.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A Lactobacillus rhamnosus strain induces a heme oxygenase dependent increase in Foxp3+ regulatory T cells.
AU - Karimi, Khalil
AU - Kandiah, Nalaayini
AU - Chau, Jessie
AU - Bienenstock, John
AU - Forsythe, Paul
PY - 2012
Y1 - 2012
N2 - We investigated the consequences of feeding with a Lactobacillus species on the immune environment in GALT, and the role of dendritic cells and heme oxygenase-1 in mediating these responses. Feeding with a specific strain of Lactobacillus rhamnosus induced a significant increase in CD4+CD25+Foxp3+ functional regulatory T cells in GALT. This increase was greatest in the mesenteric lymph nodes and associated with a marked decrease in TNF and IFN? production. Dendritic cell regulatory function and HO-1 expression was also increased. The increase in Foxp3+ T cells could be prevented by treatment with a heme oxygenase inhibitor. However, neither inhibition of heme oxygenase nor blockade of IL-10 and TGF? prevented the inhibition of inflammatory cytokine production. In conclusion Lactobacillus feeding induced a tolerogenic environment in GALT. HO-1 was critical to the enhancement of Foxp3+ regulatory T cells while additional, as yet unknown, pathways were involved in the down-regulation of inflammatory cytokine production by T cells.
AB - We investigated the consequences of feeding with a Lactobacillus species on the immune environment in GALT, and the role of dendritic cells and heme oxygenase-1 in mediating these responses. Feeding with a specific strain of Lactobacillus rhamnosus induced a significant increase in CD4+CD25+Foxp3+ functional regulatory T cells in GALT. This increase was greatest in the mesenteric lymph nodes and associated with a marked decrease in TNF and IFN? production. Dendritic cell regulatory function and HO-1 expression was also increased. The increase in Foxp3+ T cells could be prevented by treatment with a heme oxygenase inhibitor. However, neither inhibition of heme oxygenase nor blockade of IL-10 and TGF? prevented the inhibition of inflammatory cytokine production. In conclusion Lactobacillus feeding induced a tolerogenic environment in GALT. HO-1 was critical to the enhancement of Foxp3+ regulatory T cells while additional, as yet unknown, pathways were involved in the down-regulation of inflammatory cytokine production by T cells.
KW - Animals
KW - Gene Expression Regulation
KW - Mice
KW - Cell Proliferation
KW - Dendritic Cells/immunology/metabolism/microbiology
KW - Forkhead Transcription Factors/genetics/immunology/metabolism
KW - Heme Oxygenase (Decyclizing)/antagonists & inhibitors/metabolism
KW - Lactobacillus rhamnosus/pathogenicity
KW - Lymph Nodes/immunology/metabolism/pathology
KW - Mice, Transgenic/microbiology
KW - T-Lymphocytes, Regulatory/cytology/metabolism/microbiology
KW - Animals
KW - Gene Expression Regulation
KW - Mice
KW - Cell Proliferation
KW - Dendritic Cells/immunology/metabolism/microbiology
KW - Forkhead Transcription Factors/genetics/immunology/metabolism
KW - Heme Oxygenase (Decyclizing)/antagonists & inhibitors/metabolism
KW - Lactobacillus rhamnosus/pathogenicity
KW - Lymph Nodes/immunology/metabolism/pathology
KW - Mice, Transgenic/microbiology
KW - T-Lymphocytes, Regulatory/cytology/metabolism/microbiology
M3 - SCORING: Journal article
VL - 7
SP - 47556
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 10
M1 - 10
ER -
