A fluorescence diagnostic system detecting cancer-specific enzymatic activities: preliminary results

TY - CHAP

T1 - A fluorescence diagnostic system detecting cancer-specific enzymatic activities: preliminary results

T2 - Advanced Biomedical and Clinical Diagnostic Systems Vii

AU - Beck, T.

AU - Stepp, H.

AU - Wittig, R.

AU - Bohl, M.

AU - Schubert, P.

AU - Henkenjohann, S.

AU - Sauer, M.

AU - Durkop, A.

AU - Betz, C.

AU - Johansson, A.

N1 - Beck, T. Stepp, H. Wittig, R. Boehl, M. Schubert, P. Henkenjohann, S. Sauer, M. Duerkop, A. Betz, C. Johansson, A. Conference on Advanced Biomedical and Clinical Diagnostic Systems VII Jan 25-26, 2009 San Jose, CA Spie

PY - 2009

Y1 - 2009

N2 - Colorectal carcinoma is one of the most frequent and deadliest tumors in the western world. The visualization of cancer-specific enzymatic activities could possibly improve sensitivity and specificity as compared to classical white-light endoscopy. DNase X, which is typically found in early lesions, and TKTL1, which identifies aggressive carcinomas with a high metastatic potential, could potentially constitute such cancer-specific enzymes. Here, fluorescent dyes have been developed in order to specifically detect these enzymatic activities. A fiber-based system was developed for the detection of small concentrations of fluorescent dyes in scattering and absorbing media. With the use of the reflectance spectrum and a theoretical model for the light distribution, the intrinsic fluorescence is assessed from the raw fluorescence. The resulting intrinsic spectrum shows only a weak dependence on the optical properties of the sample and its intensity correlates with the fluorophore concentration. Thus, small concentrations and small variations in the concentrations of the fluorescent dye can be measured. In conclusion, the presented fluorescence diagnostic system in combination with new fluorescent probes has the potential to distinguish between cancerous tissue samples with high enzymatic activity and non-cancerous tissue samples with lower enzymatic activity.

AB - Colorectal carcinoma is one of the most frequent and deadliest tumors in the western world. The visualization of cancer-specific enzymatic activities could possibly improve sensitivity and specificity as compared to classical white-light endoscopy. DNase X, which is typically found in early lesions, and TKTL1, which identifies aggressive carcinomas with a high metastatic potential, could potentially constitute such cancer-specific enzymes. Here, fluorescent dyes have been developed in order to specifically detect these enzymatic activities. A fiber-based system was developed for the detection of small concentrations of fluorescent dyes in scattering and absorbing media. With the use of the reflectance spectrum and a theoretical model for the light distribution, the intrinsic fluorescence is assessed from the raw fluorescence. The resulting intrinsic spectrum shows only a weak dependence on the optical properties of the sample and its intensity correlates with the fluorophore concentration. Thus, small concentrations and small variations in the concentrations of the fluorescent dye can be measured. In conclusion, the presented fluorescence diagnostic system in combination with new fluorescent probes has the potential to distinguish between cancerous tissue samples with high enzymatic activity and non-cancerous tissue samples with lower enzymatic activity.

U2 - 10.1117/12.812958

DO - 10.1117/12.812958

M3 - SCORING: Beitrag in Sammelwerk

SN - 0277-786X 978-0-8194-7415-5

VL - 7169

T3 - Proceedings of SPIE

BT - https://spie.org/Publications/Proceedings/Volume/7169

A2 - MahadevanJansen, A.

A2 - VoDinh, T.

A2 - Grundfest, W. S.

ER -