A family of putative chloride channels from Arabidopsis and functional complementation of a yeast strain with a CLC gene disruption
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A family of putative chloride channels from Arabidopsis and functional complementation of a yeast strain with a CLC gene disruption. / Hechenberger, M; Schwappach, B; Fischer, W N; Frommer, W B; Jentsch, T J; Steinmeyer, K.
In: J BIOL CHEM, Vol. 271, No. 52, 27.12.1996, p. 33632-8.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - A family of putative chloride channels from Arabidopsis and functional complementation of a yeast strain with a CLC gene disruption
AU - Hechenberger, M
AU - Schwappach, B
AU - Fischer, W N
AU - Frommer, W B
AU - Jentsch, T J
AU - Steinmeyer, K
PY - 1996/12/27
Y1 - 1996/12/27
N2 - We have cloned four novel members of the CLC family of chloride channels from Arabidopsis thaliana. The four plant genes are homologous to a recently isolated chloride channel gene from tobacco (CLC-Nt1; Lurin, C., Geelen, D., Barbier-Brygoo, H., Guern, J., and Maurel, C. (1996) Plant Cell 8, 701-711) and are about 30% identical in sequence to the most closely related CLC-6 and CLC-7 putative chloride channels from mammalia. AtCLC transcripts are broadly expressed in the plant. Similarly, antibodies against the AtCLC-d protein detected the protein in all tissues, but predominantly in the silique. AtCLC-a and AtCLC-b are highly homologous to each other ( approximately 87% identity), while being approximately 50% identical to either AtCLC-c or AtCLC-d. None of the four cDNAs elicited chloride currents when expressed in Xenopus oocytes, either singly or in combination. Among these genes, only AtCLC-d could functionally substitute for the single yeast CLC protein, restoring iron-limited growth of a strain disrupted for this gene. Introduction of disease causing mutations, identified in human CLC genes, abolished this capacity. Consistent with a similar function of both proteins, the green fluorescent protein-tagged AtCLC-d protein showed the identical localization pattern as the yeast ScCLC protein. This suggests that in Arabidopsis AtCLC-d functions as an intracellular chloride channel.
AB - We have cloned four novel members of the CLC family of chloride channels from Arabidopsis thaliana. The four plant genes are homologous to a recently isolated chloride channel gene from tobacco (CLC-Nt1; Lurin, C., Geelen, D., Barbier-Brygoo, H., Guern, J., and Maurel, C. (1996) Plant Cell 8, 701-711) and are about 30% identical in sequence to the most closely related CLC-6 and CLC-7 putative chloride channels from mammalia. AtCLC transcripts are broadly expressed in the plant. Similarly, antibodies against the AtCLC-d protein detected the protein in all tissues, but predominantly in the silique. AtCLC-a and AtCLC-b are highly homologous to each other ( approximately 87% identity), while being approximately 50% identical to either AtCLC-c or AtCLC-d. None of the four cDNAs elicited chloride currents when expressed in Xenopus oocytes, either singly or in combination. Among these genes, only AtCLC-d could functionally substitute for the single yeast CLC protein, restoring iron-limited growth of a strain disrupted for this gene. Introduction of disease causing mutations, identified in human CLC genes, abolished this capacity. Consistent with a similar function of both proteins, the green fluorescent protein-tagged AtCLC-d protein showed the identical localization pattern as the yeast ScCLC protein. This suggests that in Arabidopsis AtCLC-d functions as an intracellular chloride channel.
KW - Amino Acid Sequence
KW - Animals
KW - Arabidopsis/genetics
KW - Chloride Channels/chemistry
KW - Consensus Sequence
KW - DNA, Complementary/genetics
KW - DNA, Plant/chemistry
KW - Genes, Plant
KW - Genetic Complementation Test
KW - Humans
KW - Molecular Sequence Data
KW - Muscle Proteins/chemistry
KW - Polymerase Chain Reaction
KW - Transfection
KW - Xenopus laevis
U2 - 10.1074/jbc.271.52.33632
DO - 10.1074/jbc.271.52.33632
M3 - SCORING: Journal article
C2 - 8969232
VL - 271
SP - 33632
EP - 33638
JO - J BIOL CHEM
JF - J BIOL CHEM
SN - 0021-9258
IS - 52
ER -