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A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin

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A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. / Herbig, Michael E; Houdek, Pia; Gorissen, Sascha; Zorn-Kruppa, Michaela; Wladykowski, Ewa; Volksdorf, Thomas; Grzybowski, Stephan; Kolios, Georgios; Willers, Christoph; Mallwitz, Henning; Moll, Ingrid; Brandner, Johanna M.

In: EUR J PHARM BIOPHARM, Vol. 95, 06.04.2015, p. 99-109.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Herbig, ME, Houdek, P, Gorissen, S, Zorn-Kruppa, M, Wladykowski, E, Volksdorf, T, Grzybowski, S, Kolios, G, Willers, C, Mallwitz, H, Moll, I & Brandner, JM 2015, 'A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin', EUR J PHARM BIOPHARM, vol. 95, pp. 99-109. https://doi.org/10.1016/j.ejpb.2015.03.030

APA

Herbig, M. E., Houdek, P., Gorissen, S., Zorn-Kruppa, M., Wladykowski, E., Volksdorf, T., Grzybowski, S., Kolios, G., Willers, C., Mallwitz, H., Moll, I., & Brandner, J. M. (2015). A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. EUR J PHARM BIOPHARM, 95, 99-109. https://doi.org/10.1016/j.ejpb.2015.03.030

Vancouver

Herbig ME, Houdek P, Gorissen S, Zorn-Kruppa M, Wladykowski E, Volksdorf T et al. A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin. EUR J PHARM BIOPHARM. 2015 Apr 6;95:99-109. https://doi.org/10.1016/j.ejpb.2015.03.030

Bibtex

@article{b6acdda0d99843549c5e0aefcd59dce7,
title = "A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin",
abstract = "Reliable models for the determination of skin penetration and permeation are important for the development of new drugs and formulations. The intention of our study was to develop a skin penetration model which (1) is viable and well supplied with nutrients during the period of the experiment (2) is mimicking human skin as far as possible, but still is independent from the problems of supply and heterogeneity, (3) can give information about the penetration into different compartments of the skin and (4) considers specific inter-individual differences in skin thickness. In addition, it should be quick and inexpensive (5) and without ethical implications (6). Using a chemically divers set of four topically approved active pharmaceutical ingredients (APIs), namely diclofenac, metronidazole, tazarotene, and terbinafine, we demonstrated that the model allows reliable determination of drug concentrations in different layers of the viable epidermis and dermis. For APIs susceptible for skin metabolism, the extent of metabolic transformation in epidermis and dermis can be monitored. Furthermore, a high degree of accordanceintheabilityfordiscriminationofskinconcentrationsofthesubstancesindifferentlayerswasfoundinmodelsderivedfromporcineandhumanskin. Viability, proliferation, differentiation and markers for skin barrier function were surveyed in the model. This model, which we call 'Hamburg model of skin penetration' is particularly suited to support a rational ranking and selection of dermatological formulations within drug development projects.",
author = "Herbig, {Michael E} and Pia Houdek and Sascha Gorissen and Michaela Zorn-Kruppa and Ewa Wladykowski and Thomas Volksdorf and Stephan Grzybowski and Georgios Kolios and Christoph Willers and Henning Mallwitz and Ingrid Moll and Brandner, {Johanna M}",
note = "Copyright {\textcopyright} 2015. Published by Elsevier B.V.",
year = "2015",
month = apr,
day = "6",
doi = "10.1016/j.ejpb.2015.03.030",
language = "English",
volume = "95",
pages = "99--109",
journal = "EUR J PHARM BIOPHARM",
issn = "0939-6411",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - A custom tailored model to investigate skin penetration in porcine skin and its comparison with human skin

AU - Herbig, Michael E

AU - Houdek, Pia

AU - Gorissen, Sascha

AU - Zorn-Kruppa, Michaela

AU - Wladykowski, Ewa

AU - Volksdorf, Thomas

AU - Grzybowski, Stephan

AU - Kolios, Georgios

AU - Willers, Christoph

AU - Mallwitz, Henning

AU - Moll, Ingrid

AU - Brandner, Johanna M

N1 - Copyright © 2015. Published by Elsevier B.V.

PY - 2015/4/6

Y1 - 2015/4/6

N2 - Reliable models for the determination of skin penetration and permeation are important for the development of new drugs and formulations. The intention of our study was to develop a skin penetration model which (1) is viable and well supplied with nutrients during the period of the experiment (2) is mimicking human skin as far as possible, but still is independent from the problems of supply and heterogeneity, (3) can give information about the penetration into different compartments of the skin and (4) considers specific inter-individual differences in skin thickness. In addition, it should be quick and inexpensive (5) and without ethical implications (6). Using a chemically divers set of four topically approved active pharmaceutical ingredients (APIs), namely diclofenac, metronidazole, tazarotene, and terbinafine, we demonstrated that the model allows reliable determination of drug concentrations in different layers of the viable epidermis and dermis. For APIs susceptible for skin metabolism, the extent of metabolic transformation in epidermis and dermis can be monitored. Furthermore, a high degree of accordanceintheabilityfordiscriminationofskinconcentrationsofthesubstancesindifferentlayerswasfoundinmodelsderivedfromporcineandhumanskin. Viability, proliferation, differentiation and markers for skin barrier function were surveyed in the model. This model, which we call 'Hamburg model of skin penetration' is particularly suited to support a rational ranking and selection of dermatological formulations within drug development projects.

AB - Reliable models for the determination of skin penetration and permeation are important for the development of new drugs and formulations. The intention of our study was to develop a skin penetration model which (1) is viable and well supplied with nutrients during the period of the experiment (2) is mimicking human skin as far as possible, but still is independent from the problems of supply and heterogeneity, (3) can give information about the penetration into different compartments of the skin and (4) considers specific inter-individual differences in skin thickness. In addition, it should be quick and inexpensive (5) and without ethical implications (6). Using a chemically divers set of four topically approved active pharmaceutical ingredients (APIs), namely diclofenac, metronidazole, tazarotene, and terbinafine, we demonstrated that the model allows reliable determination of drug concentrations in different layers of the viable epidermis and dermis. For APIs susceptible for skin metabolism, the extent of metabolic transformation in epidermis and dermis can be monitored. Furthermore, a high degree of accordanceintheabilityfordiscriminationofskinconcentrationsofthesubstancesindifferentlayerswasfoundinmodelsderivedfromporcineandhumanskin. Viability, proliferation, differentiation and markers for skin barrier function were surveyed in the model. This model, which we call 'Hamburg model of skin penetration' is particularly suited to support a rational ranking and selection of dermatological formulations within drug development projects.

U2 - 10.1016/j.ejpb.2015.03.030

DO - 10.1016/j.ejpb.2015.03.030

M3 - SCORING: Journal article

C2 - 25857837

VL - 95

SP - 99

EP - 109

JO - EUR J PHARM BIOPHARM

JF - EUR J PHARM BIOPHARM

SN - 0939-6411

ER -