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A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model

Standard

A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model. / Kesting, Marco Rainer; Wolff, Klaus-Dietrich; Mücke, Thomas; Demtroeder, Cedric; Kreutzer, Kilian; Schulte, Matthias; Jacobsen, Frank; Hirsch, Tobias; Loeffelbein, Denys John; Steinstraesser, Lars.

In: J BIOMED MATER RES B, Vol. 90, No. 2, 08.2009, p. 930-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kesting, MR, Wolff, K-D, Mücke, T, Demtroeder, C, Kreutzer, K, Schulte, M, Jacobsen, F, Hirsch, T, Loeffelbein, DJ & Steinstraesser, L 2009, 'A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model', J BIOMED MATER RES B, vol. 90, no. 2, pp. 930-8. https://doi.org/10.1002/jbm.b.31365

APA

Kesting, M. R., Wolff, K-D., Mücke, T., Demtroeder, C., Kreutzer, K., Schulte, M., Jacobsen, F., Hirsch, T., Loeffelbein, D. J., & Steinstraesser, L. (2009). A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model. J BIOMED MATER RES B, 90(2), 930-8. https://doi.org/10.1002/jbm.b.31365

Vancouver

Kesting MR, Wolff K-D, Mücke T, Demtroeder C, Kreutzer K, Schulte M et al. A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model. J BIOMED MATER RES B. 2009 Aug;90(2):930-8. https://doi.org/10.1002/jbm.b.31365

Bibtex

@article{f5c85f148df64991a5579593cd9ec18f,
title = "A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model",
abstract = "The study was performed to evaluate the suitability of glycerol-cryopreserved human amniotic membrane (HAM) as a surgical patch, far from its common use in ophthalmic surgery. In vivo experiments in rat models were performed to study the degradation patterns, biocompatibility, postoperative tissue formation and its suitability for abdominal wall closure. Degradation and thickness of the membranes were assessed over a period of 60 days after subdermal implantation of monolayer and multilayer HAM in 96 immunocompetent and immunosuppressed rats. The tissue response was mild, and histological analysis evaluated that multilayer application and immunosuppression prolonged graft survival significantly. In a second rat model, another 18 animals were monitored over a period of 28 days after abdominal wall reconstruction with multilayered HAM. Polypropylene mesh (Prolene) and polyglactin910/polydioxanon patches (Ethisorb) served as controls. Gross examination and histological analysis proved that multilayer HAM was a sufficient material for abdominal wall closure in comparison with the polypropylene mesh and was superior to the polyglactin910/polydioxanon patch. Additionally, significantly reduced postoperative intraabdominal adhesions were observed when compared to the polyglactin910/polydioxanon patch. This study demonstrates that HAM is a biocompatible, resorbable surgical patch in a rat xenotransplantation model and serves as a mechanically sufficient material for abdominal wall closure in a small animal model. These findings are encouraging and justify further research for the use of cryopreserved human amniotic membrane in soft tissue repair.",
keywords = "Abdominal Wall, Amnion, Animals, Biocompatible Materials, Cryopreservation, Female, Humans, Immune System, Immunohistochemistry, Immunosuppressive Agents, Male, Models, Animal, Placenta, Rats, Rats, Sprague-Dawley",
author = "Kesting, {Marco Rainer} and Klaus-Dietrich Wolff and Thomas M{\"u}cke and Cedric Demtroeder and Kilian Kreutzer and Matthias Schulte and Frank Jacobsen and Tobias Hirsch and Loeffelbein, {Denys John} and Lars Steinstraesser",
year = "2009",
month = aug,
doi = "10.1002/jbm.b.31365",
language = "English",
volume = "90",
pages = "930--8",
journal = "J BIOMED MATER RES B",
issn = "1552-4973",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - A bioartificial surgical patch from multilayered human amniotic membrane-In vivo investigations in a rat model

AU - Kesting, Marco Rainer

AU - Wolff, Klaus-Dietrich

AU - Mücke, Thomas

AU - Demtroeder, Cedric

AU - Kreutzer, Kilian

AU - Schulte, Matthias

AU - Jacobsen, Frank

AU - Hirsch, Tobias

AU - Loeffelbein, Denys John

AU - Steinstraesser, Lars

PY - 2009/8

Y1 - 2009/8

N2 - The study was performed to evaluate the suitability of glycerol-cryopreserved human amniotic membrane (HAM) as a surgical patch, far from its common use in ophthalmic surgery. In vivo experiments in rat models were performed to study the degradation patterns, biocompatibility, postoperative tissue formation and its suitability for abdominal wall closure. Degradation and thickness of the membranes were assessed over a period of 60 days after subdermal implantation of monolayer and multilayer HAM in 96 immunocompetent and immunosuppressed rats. The tissue response was mild, and histological analysis evaluated that multilayer application and immunosuppression prolonged graft survival significantly. In a second rat model, another 18 animals were monitored over a period of 28 days after abdominal wall reconstruction with multilayered HAM. Polypropylene mesh (Prolene) and polyglactin910/polydioxanon patches (Ethisorb) served as controls. Gross examination and histological analysis proved that multilayer HAM was a sufficient material for abdominal wall closure in comparison with the polypropylene mesh and was superior to the polyglactin910/polydioxanon patch. Additionally, significantly reduced postoperative intraabdominal adhesions were observed when compared to the polyglactin910/polydioxanon patch. This study demonstrates that HAM is a biocompatible, resorbable surgical patch in a rat xenotransplantation model and serves as a mechanically sufficient material for abdominal wall closure in a small animal model. These findings are encouraging and justify further research for the use of cryopreserved human amniotic membrane in soft tissue repair.

AB - The study was performed to evaluate the suitability of glycerol-cryopreserved human amniotic membrane (HAM) as a surgical patch, far from its common use in ophthalmic surgery. In vivo experiments in rat models were performed to study the degradation patterns, biocompatibility, postoperative tissue formation and its suitability for abdominal wall closure. Degradation and thickness of the membranes were assessed over a period of 60 days after subdermal implantation of monolayer and multilayer HAM in 96 immunocompetent and immunosuppressed rats. The tissue response was mild, and histological analysis evaluated that multilayer application and immunosuppression prolonged graft survival significantly. In a second rat model, another 18 animals were monitored over a period of 28 days after abdominal wall reconstruction with multilayered HAM. Polypropylene mesh (Prolene) and polyglactin910/polydioxanon patches (Ethisorb) served as controls. Gross examination and histological analysis proved that multilayer HAM was a sufficient material for abdominal wall closure in comparison with the polypropylene mesh and was superior to the polyglactin910/polydioxanon patch. Additionally, significantly reduced postoperative intraabdominal adhesions were observed when compared to the polyglactin910/polydioxanon patch. This study demonstrates that HAM is a biocompatible, resorbable surgical patch in a rat xenotransplantation model and serves as a mechanically sufficient material for abdominal wall closure in a small animal model. These findings are encouraging and justify further research for the use of cryopreserved human amniotic membrane in soft tissue repair.

KW - Abdominal Wall

KW - Amnion

KW - Animals

KW - Biocompatible Materials

KW - Cryopreservation

KW - Female

KW - Humans

KW - Immune System

KW - Immunohistochemistry

KW - Immunosuppressive Agents

KW - Male

KW - Models, Animal

KW - Placenta

KW - Rats

KW - Rats, Sprague-Dawley

U2 - 10.1002/jbm.b.31365

DO - 10.1002/jbm.b.31365

M3 - SCORING: Journal article

C2 - 19441115

VL - 90

SP - 930

EP - 938

JO - J BIOMED MATER RES B

JF - J BIOMED MATER RES B

SN - 1552-4973

IS - 2

ER -