2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS)
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2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS). / Vom Scheidt, Annika; Krug, Johannes; Goggin, Patricia; Bakker, Astrid Diana; Busse, Björn.
In: CURR OSTEOPOROS REP, Vol. 22, No. 4, 08.2024, p. 396-415.Research output: SCORING: Contribution to journal › SCORING: Review article › Research
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TY - JOUR
T1 - 2D vs. 3D Evaluation of Osteocyte Lacunae - Methodological Approaches, Recommended Parameters, and Challenges: A Narrative Review by the European Calcified Tissue Society (ECTS)
AU - Vom Scheidt, Annika
AU - Krug, Johannes
AU - Goggin, Patricia
AU - Bakker, Astrid Diana
AU - Busse, Björn
N1 - © 2024. The Author(s).
PY - 2024/8
Y1 - 2024/8
N2 - PURPOSE OF REVIEW: Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2D and 3D imaging methods for the determination of lacunar shape, orientation, density, and volume. Deviations between 2D-based and 3D-based lacunar volume estimations are often not sufficiently addressed and may give rise to contradictory findings. Thus, the systematic error arising from 2D-based estimations of lacunar volume will be discussed, and an alternative calculation proposed. Further, standardized morphological parameters and best practices for sampling and segmentation are suggested.RECENT FINDINGS: We quantified the errors in reported estimation methods of lacunar volume based on 2D cross-sections, which increase with variations in lacunar orientation and histological cutting plane. The estimations of lacunar volume based on common practice in 2D imaging methods resulted in an underestimation of lacunar volume of up to 85% compared to actual lacunar volume in an artificial dataset. For a representative estimation of lacunar size and morphology based on 2D images, at least 400 lacunae should be assessed per sample.
AB - PURPOSE OF REVIEW: Quantification of the morphology of osteocyte lacunae has become a powerful tool to investigate bone metabolism, pathologies and aging. This review will provide a brief overview of 2D and 3D imaging methods for the determination of lacunar shape, orientation, density, and volume. Deviations between 2D-based and 3D-based lacunar volume estimations are often not sufficiently addressed and may give rise to contradictory findings. Thus, the systematic error arising from 2D-based estimations of lacunar volume will be discussed, and an alternative calculation proposed. Further, standardized morphological parameters and best practices for sampling and segmentation are suggested.RECENT FINDINGS: We quantified the errors in reported estimation methods of lacunar volume based on 2D cross-sections, which increase with variations in lacunar orientation and histological cutting plane. The estimations of lacunar volume based on common practice in 2D imaging methods resulted in an underestimation of lacunar volume of up to 85% compared to actual lacunar volume in an artificial dataset. For a representative estimation of lacunar size and morphology based on 2D images, at least 400 lacunae should be assessed per sample.
KW - Humans
KW - Osteocytes/cytology
KW - Imaging, Three-Dimensional/methods
KW - Bone Density
KW - Bone and Bones/diagnostic imaging
KW - Image Processing, Computer-Assisted/methods
U2 - 10.1007/s11914-024-00877-z
DO - 10.1007/s11914-024-00877-z
M3 - SCORING: Review article
C2 - 38980532
VL - 22
SP - 396
EP - 415
JO - CURR OSTEOPOROS REP
JF - CURR OSTEOPOROS REP
SN - 1544-1873
IS - 4
ER -
