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16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population

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16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population. / Rezasoltani, Sama; Azizmohammad Looha, Mehdi; Asadzadeh Aghdaei, Hamid; Jasemi, Seyedesomayeh; Sechi, Leonardo Antonio; Gazouli, Maria; Sadeghi, Amir; Torkashvand, Shirin; Baniali, Reyhaneh; Schlüter, Hartmut; Zali, Mohammad Reza; Feizabadi, Mohammad Mehdi.

In: GUT PATHOG, Vol. 16, No. 1, 20.02.2024, p. 9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rezasoltani, S, Azizmohammad Looha, M, Asadzadeh Aghdaei, H, Jasemi, S, Sechi, LA, Gazouli, M, Sadeghi, A, Torkashvand, S, Baniali, R, Schlüter, H, Zali, MR & Feizabadi, MM 2024, '16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population', GUT PATHOG, vol. 16, no. 1, pp. 9. https://doi.org/10.1186/s13099-024-00604-0

APA

Rezasoltani, S., Azizmohammad Looha, M., Asadzadeh Aghdaei, H., Jasemi, S., Sechi, L. A., Gazouli, M., Sadeghi, A., Torkashvand, S., Baniali, R., Schlüter, H., Zali, M. R., & Feizabadi, M. M. (2024). 16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population. GUT PATHOG, 16(1), 9. https://doi.org/10.1186/s13099-024-00604-0

Vancouver

Rezasoltani S, Azizmohammad Looha M, Asadzadeh Aghdaei H, Jasemi S, Sechi LA, Gazouli M et al. 16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population. GUT PATHOG. 2024 Feb 20;16(1):9. https://doi.org/10.1186/s13099-024-00604-0

Bibtex

@article{59f7f27b683648c58b576764339703e8,
title = "16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population",
abstract = "BACKGROUND: Colorectal cancer (CRC) poses a significant healthcare challenge, accounting for nearly 6.1% of global cancer cases. Early detection, facilitated by population screening utilizing innovative biomarkers, is pivotal for mitigating CRC incidence. This study aims to scrutinize the fecal and salivary microbiomes of CRC-positive individuals (CPs) in comparison to CRC-negative counterparts (CNs) to enhance early CRC diagnosis through microbial biomarkers.MATERIAL AND METHODS: A total of 80 oral and stool samples were collected from Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran, encompassing both CPs and CNs undergoing screening. Microbial profiling was conducted using 16S rRNA sequencing assays, employing the Nextera XT Index Kit on an Illumina NovaSeq platform.RESULTS: Distinct microbial profiles were observed in saliva and stool samples of CPs, diverging significantly from those of CNs at various taxonomic levels, including phylum, family, and species. Saliva samples from CPs exhibited abundance of Calothrix parietina, Granulicatella adiacens, Rothia dentocariosa, and Rothia mucilaginosa, absent in CNs. Additionally, Lachnospiraceae and Prevotellaceae were markedly higher in CPs' feces, while the Fusobacteria phylum was significantly elevated in CPs' saliva. Conversely, the non-pathogenic bacterium Akkermansia muciniphila exhibited a significant decrease in CPs' fecal samples compared to CNs.CONCLUSION: Through meticulous selection of saliva and stool microbes based on Mean Decrease GINI values and employing logistic regression for saliva and support vector machine models for stool, we successfully developed a microbiota test with heightened sensitivity and specificity for early CRC detection.",
author = "Sama Rezasoltani and {Azizmohammad Looha}, Mehdi and {Asadzadeh Aghdaei}, Hamid and Seyedesomayeh Jasemi and Sechi, {Leonardo Antonio} and Maria Gazouli and Amir Sadeghi and Shirin Torkashvand and Reyhaneh Baniali and Hartmut Schl{\"u}ter and Zali, {Mohammad Reza} and Feizabadi, {Mohammad Mehdi}",
note = "{\textcopyright} 2024. The Author(s).",
year = "2024",
month = feb,
day = "20",
doi = "10.1186/s13099-024-00604-0",
language = "English",
volume = "16",
pages = "9",
journal = "GUT PATHOG",
issn = "1757-4749",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - 16S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population

AU - Rezasoltani, Sama

AU - Azizmohammad Looha, Mehdi

AU - Asadzadeh Aghdaei, Hamid

AU - Jasemi, Seyedesomayeh

AU - Sechi, Leonardo Antonio

AU - Gazouli, Maria

AU - Sadeghi, Amir

AU - Torkashvand, Shirin

AU - Baniali, Reyhaneh

AU - Schlüter, Hartmut

AU - Zali, Mohammad Reza

AU - Feizabadi, Mohammad Mehdi

N1 - © 2024. The Author(s).

PY - 2024/2/20

Y1 - 2024/2/20

N2 - BACKGROUND: Colorectal cancer (CRC) poses a significant healthcare challenge, accounting for nearly 6.1% of global cancer cases. Early detection, facilitated by population screening utilizing innovative biomarkers, is pivotal for mitigating CRC incidence. This study aims to scrutinize the fecal and salivary microbiomes of CRC-positive individuals (CPs) in comparison to CRC-negative counterparts (CNs) to enhance early CRC diagnosis through microbial biomarkers.MATERIAL AND METHODS: A total of 80 oral and stool samples were collected from Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran, encompassing both CPs and CNs undergoing screening. Microbial profiling was conducted using 16S rRNA sequencing assays, employing the Nextera XT Index Kit on an Illumina NovaSeq platform.RESULTS: Distinct microbial profiles were observed in saliva and stool samples of CPs, diverging significantly from those of CNs at various taxonomic levels, including phylum, family, and species. Saliva samples from CPs exhibited abundance of Calothrix parietina, Granulicatella adiacens, Rothia dentocariosa, and Rothia mucilaginosa, absent in CNs. Additionally, Lachnospiraceae and Prevotellaceae were markedly higher in CPs' feces, while the Fusobacteria phylum was significantly elevated in CPs' saliva. Conversely, the non-pathogenic bacterium Akkermansia muciniphila exhibited a significant decrease in CPs' fecal samples compared to CNs.CONCLUSION: Through meticulous selection of saliva and stool microbes based on Mean Decrease GINI values and employing logistic regression for saliva and support vector machine models for stool, we successfully developed a microbiota test with heightened sensitivity and specificity for early CRC detection.

AB - BACKGROUND: Colorectal cancer (CRC) poses a significant healthcare challenge, accounting for nearly 6.1% of global cancer cases. Early detection, facilitated by population screening utilizing innovative biomarkers, is pivotal for mitigating CRC incidence. This study aims to scrutinize the fecal and salivary microbiomes of CRC-positive individuals (CPs) in comparison to CRC-negative counterparts (CNs) to enhance early CRC diagnosis through microbial biomarkers.MATERIAL AND METHODS: A total of 80 oral and stool samples were collected from Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran, encompassing both CPs and CNs undergoing screening. Microbial profiling was conducted using 16S rRNA sequencing assays, employing the Nextera XT Index Kit on an Illumina NovaSeq platform.RESULTS: Distinct microbial profiles were observed in saliva and stool samples of CPs, diverging significantly from those of CNs at various taxonomic levels, including phylum, family, and species. Saliva samples from CPs exhibited abundance of Calothrix parietina, Granulicatella adiacens, Rothia dentocariosa, and Rothia mucilaginosa, absent in CNs. Additionally, Lachnospiraceae and Prevotellaceae were markedly higher in CPs' feces, while the Fusobacteria phylum was significantly elevated in CPs' saliva. Conversely, the non-pathogenic bacterium Akkermansia muciniphila exhibited a significant decrease in CPs' fecal samples compared to CNs.CONCLUSION: Through meticulous selection of saliva and stool microbes based on Mean Decrease GINI values and employing logistic regression for saliva and support vector machine models for stool, we successfully developed a microbiota test with heightened sensitivity and specificity for early CRC detection.

U2 - 10.1186/s13099-024-00604-0

DO - 10.1186/s13099-024-00604-0

M3 - SCORING: Journal article

C2 - 38378690

VL - 16

SP - 9

JO - GUT PATHOG

JF - GUT PATHOG

SN - 1757-4749

IS - 1

ER -