Wnt1 overexpression leads to enforced cardiomyogenesis and inhibition of hematopoiesis in murine embryonic stem cells
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Wnt1 overexpression leads to enforced cardiomyogenesis and inhibition of hematopoiesis in murine embryonic stem cells. / Weisel, Katja C; Kopp, Hans-Georg; Moore, Malcolm A S; Studer, Lorenz; Barberi, Tiziano.
in: STEM CELLS DEV, Jahrgang 19, Nr. 5, 05.2010, S. 745-51.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Wnt1 overexpression leads to enforced cardiomyogenesis and inhibition of hematopoiesis in murine embryonic stem cells
AU - Weisel, Katja C
AU - Kopp, Hans-Georg
AU - Moore, Malcolm A S
AU - Studer, Lorenz
AU - Barberi, Tiziano
PY - 2010/5
Y1 - 2010/5
N2 - Recent findings emphasized a critical role for the Wnt signaling pathway during the early steps of embryogenesis, including the development of the hematopoietic system and cardiac development. To date, the role of Wnt in promoting or inhibiting development of both tissues was discussed controversially, dependent on species and time point of expression. Differentiation of embryonic stem cells (ESC) recapitulates early stages of mammalian development. In the present study, we generated murine ESC lines overexpressing Wnt1 (Wnt1 ES). When induced to differentiate toward the cardiomyocytic lineage, Wnt1 ES showed a significant increased ability to generate cardiomyocytes when compared with a control ESC (control ES) line. In addition, Wnt1 ES cells were unable to form hematopoietic cells, whereas development of endothelial cells, a cell type closely associated with blood during embryogenesis, was comparable to control ES. Finally, cardiac differentiation was markedly decreased by the addition of the Wnt antagonist Dkk-1 to the culture medium. These findings suggest that Wnt1 may regulate differentiation of immature mesodermal cells in a tissue-specific manner.
AB - Recent findings emphasized a critical role for the Wnt signaling pathway during the early steps of embryogenesis, including the development of the hematopoietic system and cardiac development. To date, the role of Wnt in promoting or inhibiting development of both tissues was discussed controversially, dependent on species and time point of expression. Differentiation of embryonic stem cells (ESC) recapitulates early stages of mammalian development. In the present study, we generated murine ESC lines overexpressing Wnt1 (Wnt1 ES). When induced to differentiate toward the cardiomyocytic lineage, Wnt1 ES showed a significant increased ability to generate cardiomyocytes when compared with a control ESC (control ES) line. In addition, Wnt1 ES cells were unable to form hematopoietic cells, whereas development of endothelial cells, a cell type closely associated with blood during embryogenesis, was comparable to control ES. Finally, cardiac differentiation was markedly decreased by the addition of the Wnt antagonist Dkk-1 to the culture medium. These findings suggest that Wnt1 may regulate differentiation of immature mesodermal cells in a tissue-specific manner.
KW - Animals
KW - Cell Differentiation
KW - Cell Line
KW - Cell Lineage
KW - Embryonic Stem Cells
KW - Hematopoiesis
KW - Mice
KW - Muscle Development
KW - Myocytes, Cardiac
KW - Wnt1 Protein
KW - Journal Article
U2 - 10.1089/scd.2008.0356
DO - 10.1089/scd.2008.0356
M3 - SCORING: Journal article
C2 - 19422288
VL - 19
SP - 745
EP - 751
JO - STEM CELLS DEV
JF - STEM CELLS DEV
SN - 1547-3287
IS - 5
ER -
