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Widespread transcription at neuronal activity-regulated enhancers.

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Widespread transcription at neuronal activity-regulated enhancers. / Kim, Tae-Kyung; Hemberg, Martin; Gray, Jesse M; Costa, Allen M; Bear, Daniel M; Wu, Jing; Harmin, David A; Laptewicz, Mike; Barbara-Haley, Kellie; Kuersten, Scott; Markenscoff-Papadimitriou, Eirene; Kuhl, Dietmar; Bito, Haruhiko; Worley, Paul F; Kreiman, Gabriel; Greenberg, Michael E.

in: NATURE, Jahrgang 465, Nr. 7295, 7295, 2010, S. 182-187.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kim, T-K, Hemberg, M, Gray, JM, Costa, AM, Bear, DM, Wu, J, Harmin, DA, Laptewicz, M, Barbara-Haley, K, Kuersten, S, Markenscoff-Papadimitriou, E, Kuhl, D, Bito, H, Worley, PF, Kreiman, G & Greenberg, ME 2010, 'Widespread transcription at neuronal activity-regulated enhancers.', NATURE, Jg. 465, Nr. 7295, 7295, S. 182-187. <http://www.ncbi.nlm.nih.gov/pubmed/20393465?dopt=Citation>

APA

Kim, T-K., Hemberg, M., Gray, J. M., Costa, A. M., Bear, D. M., Wu, J., Harmin, D. A., Laptewicz, M., Barbara-Haley, K., Kuersten, S., Markenscoff-Papadimitriou, E., Kuhl, D., Bito, H., Worley, P. F., Kreiman, G., & Greenberg, M. E. (2010). Widespread transcription at neuronal activity-regulated enhancers. NATURE, 465(7295), 182-187. [7295]. http://www.ncbi.nlm.nih.gov/pubmed/20393465?dopt=Citation

Vancouver

Kim T-K, Hemberg M, Gray JM, Costa AM, Bear DM, Wu J et al. Widespread transcription at neuronal activity-regulated enhancers. NATURE. 2010;465(7295):182-187. 7295.

Bibtex

@article{3a9f5b14d6f14a559119cfcc7caaadcd,
title = "Widespread transcription at neuronal activity-regulated enhancers.",
abstract = "We used genome-wide sequencing methods to study stimulus-dependent enhancer function in mouse cortical neurons. We identified approximately 12,000 neuronal activity-regulated enhancers that are bound by the general transcriptional co-activator CBP in an activity-dependent manner. A function of CBP at enhancers may be to recruit RNA polymerase II (RNAPII), as we also observed activity-regulated RNAPII binding to thousands of enhancers. Notably, RNAPII at enhancers transcribes bi-directionally a novel class of enhancer RNAs (eRNAs) within enhancer domains defined by the presence of histone H3 monomethylated at lysine 4. The level of eRNA expression at neuronal enhancers positively correlates with the level of messenger RNA synthesis at nearby genes, suggesting that eRNA synthesis occurs specifically at enhancers that are actively engaged in promoting mRNA synthesis. These findings reveal that a widespread mechanism of enhancer activation involves RNAPII binding and eRNA synthesis.",
author = "Tae-Kyung Kim and Martin Hemberg and Gray, {Jesse M} and Costa, {Allen M} and Bear, {Daniel M} and Jing Wu and Harmin, {David A} and Mike Laptewicz and Kellie Barbara-Haley and Scott Kuersten and Eirene Markenscoff-Papadimitriou and Dietmar Kuhl and Haruhiko Bito and Worley, {Paul F} and Gabriel Kreiman and Greenberg, {Michael E}",
year = "2010",
language = "Deutsch",
volume = "465",
pages = "182--187",
journal = "NATURE",
issn = "0028-0836",
publisher = "NATURE PUBLISHING GROUP",
number = "7295",

}

RIS

TY - JOUR

T1 - Widespread transcription at neuronal activity-regulated enhancers.

AU - Kim, Tae-Kyung

AU - Hemberg, Martin

AU - Gray, Jesse M

AU - Costa, Allen M

AU - Bear, Daniel M

AU - Wu, Jing

AU - Harmin, David A

AU - Laptewicz, Mike

AU - Barbara-Haley, Kellie

AU - Kuersten, Scott

AU - Markenscoff-Papadimitriou, Eirene

AU - Kuhl, Dietmar

AU - Bito, Haruhiko

AU - Worley, Paul F

AU - Kreiman, Gabriel

AU - Greenberg, Michael E

PY - 2010

Y1 - 2010

N2 - We used genome-wide sequencing methods to study stimulus-dependent enhancer function in mouse cortical neurons. We identified approximately 12,000 neuronal activity-regulated enhancers that are bound by the general transcriptional co-activator CBP in an activity-dependent manner. A function of CBP at enhancers may be to recruit RNA polymerase II (RNAPII), as we also observed activity-regulated RNAPII binding to thousands of enhancers. Notably, RNAPII at enhancers transcribes bi-directionally a novel class of enhancer RNAs (eRNAs) within enhancer domains defined by the presence of histone H3 monomethylated at lysine 4. The level of eRNA expression at neuronal enhancers positively correlates with the level of messenger RNA synthesis at nearby genes, suggesting that eRNA synthesis occurs specifically at enhancers that are actively engaged in promoting mRNA synthesis. These findings reveal that a widespread mechanism of enhancer activation involves RNAPII binding and eRNA synthesis.

AB - We used genome-wide sequencing methods to study stimulus-dependent enhancer function in mouse cortical neurons. We identified approximately 12,000 neuronal activity-regulated enhancers that are bound by the general transcriptional co-activator CBP in an activity-dependent manner. A function of CBP at enhancers may be to recruit RNA polymerase II (RNAPII), as we also observed activity-regulated RNAPII binding to thousands of enhancers. Notably, RNAPII at enhancers transcribes bi-directionally a novel class of enhancer RNAs (eRNAs) within enhancer domains defined by the presence of histone H3 monomethylated at lysine 4. The level of eRNA expression at neuronal enhancers positively correlates with the level of messenger RNA synthesis at nearby genes, suggesting that eRNA synthesis occurs specifically at enhancers that are actively engaged in promoting mRNA synthesis. These findings reveal that a widespread mechanism of enhancer activation involves RNAPII binding and eRNA synthesis.

M3 - SCORING: Zeitschriftenaufsatz

VL - 465

SP - 182

EP - 187

JO - NATURE

JF - NATURE

SN - 0028-0836

IS - 7295

M1 - 7295

ER -