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Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells

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Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells. / Gasch, Christin; Pantel, Klaus; Riethdorf, Sabine.

in: Methods Mol Biol, Jahrgang 1347, 2015, S. 221-32.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gasch, C, Pantel, K & Riethdorf, S 2015, 'Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells', Methods Mol Biol, Jg. 1347, S. 221-32. https://doi.org/10.1007/978-1-4939-2990-0_15

APA

Gasch, C., Pantel, K., & Riethdorf, S. (2015). Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells. Methods Mol Biol, 1347, 221-32. https://doi.org/10.1007/978-1-4939-2990-0_15

Vancouver

Gasch C, Pantel K, Riethdorf S. Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells. Methods Mol Biol. 2015;1347:221-32. https://doi.org/10.1007/978-1-4939-2990-0_15

Bibtex

@article{c763a81af32b4e389ab7d3ba12a4b8a5,
title = "Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells",
abstract = "Investigation of the genome of organisms is one of the major basics in molecular biology to understand the complex organization of cells. While genomic DNA can easily be isolated from tissues or cell cultures of plant, animal or human origin, DNA extraction from single cells is still challenging. Here, we describe three techniques for the amplification of genomic DNA of fixed single circulating tumor cells (CTC) isolated from blood of cancer patients. This amplification is aimed to increase DNA amounts from those of one cell to yields sufficient for different DNA analyses such as mutational analysis including next-generation sequencing, array-comparative genome hybridization (CGH), and quantitative measurement of gene amplifications. Molecular analysis of CTC as liquid biopsy can be used to identify therapeutic targets in personalized medicine directed, e.g. against human epidermal growth factor receptor 2 (HER2) or epidermal growth factor receptor (EGFR) and to stratify the patients to those therapies.",
author = "Christin Gasch and Klaus Pantel and Sabine Riethdorf",
year = "2015",
doi = "10.1007/978-1-4939-2990-0_15",
language = "English",
volume = "1347",
pages = "221--32",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells

AU - Gasch, Christin

AU - Pantel, Klaus

AU - Riethdorf, Sabine

PY - 2015

Y1 - 2015

N2 - Investigation of the genome of organisms is one of the major basics in molecular biology to understand the complex organization of cells. While genomic DNA can easily be isolated from tissues or cell cultures of plant, animal or human origin, DNA extraction from single cells is still challenging. Here, we describe three techniques for the amplification of genomic DNA of fixed single circulating tumor cells (CTC) isolated from blood of cancer patients. This amplification is aimed to increase DNA amounts from those of one cell to yields sufficient for different DNA analyses such as mutational analysis including next-generation sequencing, array-comparative genome hybridization (CGH), and quantitative measurement of gene amplifications. Molecular analysis of CTC as liquid biopsy can be used to identify therapeutic targets in personalized medicine directed, e.g. against human epidermal growth factor receptor 2 (HER2) or epidermal growth factor receptor (EGFR) and to stratify the patients to those therapies.

AB - Investigation of the genome of organisms is one of the major basics in molecular biology to understand the complex organization of cells. While genomic DNA can easily be isolated from tissues or cell cultures of plant, animal or human origin, DNA extraction from single cells is still challenging. Here, we describe three techniques for the amplification of genomic DNA of fixed single circulating tumor cells (CTC) isolated from blood of cancer patients. This amplification is aimed to increase DNA amounts from those of one cell to yields sufficient for different DNA analyses such as mutational analysis including next-generation sequencing, array-comparative genome hybridization (CGH), and quantitative measurement of gene amplifications. Molecular analysis of CTC as liquid biopsy can be used to identify therapeutic targets in personalized medicine directed, e.g. against human epidermal growth factor receptor 2 (HER2) or epidermal growth factor receptor (EGFR) and to stratify the patients to those therapies.

U2 - 10.1007/978-1-4939-2990-0_15

DO - 10.1007/978-1-4939-2990-0_15

M3 - SCORING: Journal article

C2 - 26374320

VL - 1347

SP - 221

EP - 232

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -