When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction

Petra Clara Arck

doi:10.1016/j.jri.2019.03.004

When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction

Standard

When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction. / Arck, Petra Clara.

in: J REPROD IMMUNOL, Jahrgang 132, 04.2019, S. 54-59.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung

Harvard

Arck, PC 2019, 'When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction', J REPROD IMMUNOL, Jg. 132, S. 54-59. https://doi.org/10.1016/j.jri.2019.03.004

APA

Arck, P. C. (2019). When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction. J REPROD IMMUNOL, 132, 54-59. https://doi.org/10.1016/j.jri.2019.03.004

Vancouver

Arck PC. When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction. J REPROD IMMUNOL. 2019 Apr;132:54-59. https://doi.org/10.1016/j.jri.2019.03.004

Bibtex

@article{d14c885c9a2640df9e9265e9a11eb4c5,

title = "When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction",

abstract = "Research endeavors aiming to understand the maternal immune adaptation to pregnancy significantly rely on the use of animal models, such as mice and rats. These models have provided important insights into the pathophysiology of a number of pregnancy disorders in humans. However, the use of animal models in scientific research is a vividly debated and emotive topic. The 3R principles - replacement, reduction and refinement of research animals - have been propagated a few decades ago. The present review advocates a forward-thinking consciousness to address the 3R principles in research projects in the field of reproductive biology and immunology. Specific measures and alternative methods are being proposed to replace research animals by using e.g. tissue engineering approaches, biobank-derived tissue, 'placenta-on-a-chip' devices or in silico methods. The latter may involve data queries from repositories now available to provide single cell sequencing information on reproductive tissues. Reduction of research animals by gestational imaging and a wealth of suggestions for refinement are proposed. Taken together, the measures and guidelines introduced in this review are expected to spark a reconsideration of experimental designs in the area of reproductive biology and immunology in order to implement 3R principle where applicable.",

keywords = "Journal Article, Review",

author = "Arck, {Petra Clara}",

year = "2019",

month = apr,

doi = "10.1016/j.jri.2019.03.004",

language = "English",

volume = "132",

pages = "54--59",

journal = "J REPROD IMMUNOL",

issn = "0165-0378",

publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - When 3 Rs meet a forth R: Replacement, reduction and refinement of animals in research on reproduction

AU - Arck, Petra Clara

PY - 2019/4

Y1 - 2019/4

N2 - Research endeavors aiming to understand the maternal immune adaptation to pregnancy significantly rely on the use of animal models, such as mice and rats. These models have provided important insights into the pathophysiology of a number of pregnancy disorders in humans. However, the use of animal models in scientific research is a vividly debated and emotive topic. The 3R principles - replacement, reduction and refinement of research animals - have been propagated a few decades ago. The present review advocates a forward-thinking consciousness to address the 3R principles in research projects in the field of reproductive biology and immunology. Specific measures and alternative methods are being proposed to replace research animals by using e.g. tissue engineering approaches, biobank-derived tissue, 'placenta-on-a-chip' devices or in silico methods. The latter may involve data queries from repositories now available to provide single cell sequencing information on reproductive tissues. Reduction of research animals by gestational imaging and a wealth of suggestions for refinement are proposed. Taken together, the measures and guidelines introduced in this review are expected to spark a reconsideration of experimental designs in the area of reproductive biology and immunology in order to implement 3R principle where applicable.

AB - Research endeavors aiming to understand the maternal immune adaptation to pregnancy significantly rely on the use of animal models, such as mice and rats. These models have provided important insights into the pathophysiology of a number of pregnancy disorders in humans. However, the use of animal models in scientific research is a vividly debated and emotive topic. The 3R principles - replacement, reduction and refinement of research animals - have been propagated a few decades ago. The present review advocates a forward-thinking consciousness to address the 3R principles in research projects in the field of reproductive biology and immunology. Specific measures and alternative methods are being proposed to replace research animals by using e.g. tissue engineering approaches, biobank-derived tissue, 'placenta-on-a-chip' devices or in silico methods. The latter may involve data queries from repositories now available to provide single cell sequencing information on reproductive tissues. Reduction of research animals by gestational imaging and a wealth of suggestions for refinement are proposed. Taken together, the measures and guidelines introduced in this review are expected to spark a reconsideration of experimental designs in the area of reproductive biology and immunology in order to implement 3R principle where applicable.

KW - Journal Article

KW - Review

U2 - 10.1016/j.jri.2019.03.004

DO - 10.1016/j.jri.2019.03.004

M3 - SCORING: Review article

C2 - 30951977

VL - 132

SP - 54

EP - 59

JO - J REPROD IMMUNOL

JF - J REPROD IMMUNOL

SN - 0165-0378

ER -