What radiation dose does the FISH translocation assay measure in cases of incorporated radionuclides for the Southern Urals populations?
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What radiation dose does the FISH translocation assay measure in cases of incorporated radionuclides for the Southern Urals populations? / Ainsbury, Elizabeth A; Moquet, Jayne; Rothkamm, Kai; Darroudi, Firouz; Vozilova, Alexandra; Degteva, Marina; Azizova, Tamara V; Lloyd, David C; Harrison, John.
in: RADIAT PROT DOSIM, Jahrgang 159, Nr. 1-4, 06.2014, S. 26-33.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - What radiation dose does the FISH translocation assay measure in cases of incorporated radionuclides for the Southern Urals populations?
AU - Ainsbury, Elizabeth A
AU - Moquet, Jayne
AU - Rothkamm, Kai
AU - Darroudi, Firouz
AU - Vozilova, Alexandra
AU - Degteva, Marina
AU - Azizova, Tamara V
AU - Lloyd, David C
AU - Harrison, John
N1 - © Crown copyright 2014.
PY - 2014/6
Y1 - 2014/6
N2 - The fluorescence in situ hybridisation (FISH) technique is now well established for retrospective dosimetry in cases of external radiation exposure that occurred many years ago. However, the question remains as to whether FISH provides valid estimates of cumulative red bone marrow radiation doses in cases of incorporation of radionuclides or combined external and internal exposures. This question has arisen in connection with the interpretation of results of dose assessments for epidemiological studies of plutonium workers at the Russian Mayak plant and of members of the public exposed to strontium radioisotopes and external radiation as a result of discharges from Mayak to the Techa River. Exposures to penetrating external radiation result in fairly uniform irradiation of body tissues, and hence similar doses to all tissues, for which FISH dosimetry can provide a reliable measure of this whole body dose. However, intakes of radionuclides into the body by inhalation or ingestion may result in retention in specific organs and tissues, so that the distribution of dose is highly heterogeneous. For radionuclides emitting short-range radiations (e.g. alpha particles), this heterogeneity can apply to dose delivery within tissues and between cells within tissues. In this paper, an attempt is made to address the question of what FISH measures in such circumstances by considering evidence regarding the origin and lifetime dynamics of lymphocyte subsets in the human body in relation to the localised delivery of dose from the internal emitters (90)Sr and (239)Pu, which are of particular interest for the Southern Urals Mayak and Techa River populations, and for which most evidence is available in these populations. It is concluded that the FISH translocation assay can be usefully applied for detecting internal and combined external gamma and internal doses from internally deposited (90)Sr, albeit with fairly large uncertainties. The same may be true of (239)Pu, as well as other radionuclides, although much work remains to be done to establish dose-response relationships.
AB - The fluorescence in situ hybridisation (FISH) technique is now well established for retrospective dosimetry in cases of external radiation exposure that occurred many years ago. However, the question remains as to whether FISH provides valid estimates of cumulative red bone marrow radiation doses in cases of incorporation of radionuclides or combined external and internal exposures. This question has arisen in connection with the interpretation of results of dose assessments for epidemiological studies of plutonium workers at the Russian Mayak plant and of members of the public exposed to strontium radioisotopes and external radiation as a result of discharges from Mayak to the Techa River. Exposures to penetrating external radiation result in fairly uniform irradiation of body tissues, and hence similar doses to all tissues, for which FISH dosimetry can provide a reliable measure of this whole body dose. However, intakes of radionuclides into the body by inhalation or ingestion may result in retention in specific organs and tissues, so that the distribution of dose is highly heterogeneous. For radionuclides emitting short-range radiations (e.g. alpha particles), this heterogeneity can apply to dose delivery within tissues and between cells within tissues. In this paper, an attempt is made to address the question of what FISH measures in such circumstances by considering evidence regarding the origin and lifetime dynamics of lymphocyte subsets in the human body in relation to the localised delivery of dose from the internal emitters (90)Sr and (239)Pu, which are of particular interest for the Southern Urals Mayak and Techa River populations, and for which most evidence is available in these populations. It is concluded that the FISH translocation assay can be usefully applied for detecting internal and combined external gamma and internal doses from internally deposited (90)Sr, albeit with fairly large uncertainties. The same may be true of (239)Pu, as well as other radionuclides, although much work remains to be done to establish dose-response relationships.
KW - Humans
KW - In Situ Hybridization, Fluorescence/methods
KW - Lymphocytes/radiation effects
KW - Plutonium/analysis
KW - Radiation Dosage
KW - Radiation Monitoring/methods
KW - Relative Biological Effectiveness
KW - Rural Population
KW - Russia
KW - Strontium Radioisotopes/analysis
KW - Translocation, Genetic/genetics
U2 - 10.1093/rpd/ncu118
DO - 10.1093/rpd/ncu118
M3 - SCORING: Journal article
C2 - 24736296
VL - 159
SP - 26
EP - 33
JO - RADIAT PROT DOSIM
JF - RADIAT PROT DOSIM
SN - 0144-8420
IS - 1-4
ER -