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Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry

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Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry. / Eiberg, Samantha; Ancker, Leif; Javed, Sana; Haag, Friedrich.

The P2X7 Receptor: Methods and Protocols. Hrsg. / Annette Nicke. 1. Aufl. New York, NY : HUMANA PRESS INC, 2022. S. 265-278 (Methods in Molecular Biology; Band 2510).

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Harvard

Eiberg, S, Ancker, L, Javed, S & Haag, F 2022, Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry. in A Nicke (Hrsg.), The P2X7 Receptor: Methods and Protocols. 1 Aufl., Methods in Molecular Biology, Bd. 2510, HUMANA PRESS INC, New York, NY, S. 265-278. https://doi.org/10.1007/978-1-0716-2384-8_14

APA

Eiberg, S., Ancker, L., Javed, S., & Haag, F. (2022). Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry. in A. Nicke (Hrsg.), The P2X7 Receptor: Methods and Protocols (1 Aufl., S. 265-278). (Methods in Molecular Biology; Band 2510). HUMANA PRESS INC. https://doi.org/10.1007/978-1-0716-2384-8_14

Vancouver

Eiberg S, Ancker L, Javed S, Haag F. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry. in Nicke A, Hrsg., The P2X7 Receptor: Methods and Protocols. 1 Aufl. New York, NY: HUMANA PRESS INC. 2022. S. 265-278. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-2384-8_14

Bibtex

@inbook{7269499503724d86a80808048230ddfd,
title = "Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry",
abstract = "One of the most prominent effects of P2X7 activation in myeloid cells is the induction of the assembly of the NLRP3 inflammasome, a central process controlling the secretion of pro-inflammatory cytokines of the IL-1 family such as IL-1β and IL-18. The ability to visualize inflammasome formation greatly facilitates research into the role of P2X7 in inflammation. In this chapter, a method to monitor the formation of the NLPR3 inflammasome in monocytes and other myeloid cells could be demonstrated. Following priming by lipopolysaccharide (LPS), P2X7 was stimulated by ATP to mediate inflammasome assembly. This causes cytosolically disperse ASC, a central component of the inflammasome, to aggregate into microscopically visible specks due to its recruitment to the inflammasome. Methods to monitor this change in the spatial distribution of ASC in human peripheral blood monocytes by flow cytometry and fluorescence microscopy are presented.",
keywords = "Flow Cytometry, Humans, Inflammasomes, Lipopolysaccharides/pharmacology, Microscopy, Fluorescence, Monocytes",
author = "Samantha Eiberg and Leif Ancker and Sana Javed and Friedrich Haag",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2384-8_14",
language = "English",
isbn = "978-1-0716-2383-1",
series = "Methods in Molecular Biology",
publisher = "HUMANA PRESS INC",
pages = "265--278",
editor = "Annette Nicke",
booktitle = "The P2X7 Receptor",
edition = "1",

}

RIS

TY - CHAP

T1 - Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry

AU - Eiberg, Samantha

AU - Ancker, Leif

AU - Javed, Sana

AU - Haag, Friedrich

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - One of the most prominent effects of P2X7 activation in myeloid cells is the induction of the assembly of the NLRP3 inflammasome, a central process controlling the secretion of pro-inflammatory cytokines of the IL-1 family such as IL-1β and IL-18. The ability to visualize inflammasome formation greatly facilitates research into the role of P2X7 in inflammation. In this chapter, a method to monitor the formation of the NLPR3 inflammasome in monocytes and other myeloid cells could be demonstrated. Following priming by lipopolysaccharide (LPS), P2X7 was stimulated by ATP to mediate inflammasome assembly. This causes cytosolically disperse ASC, a central component of the inflammasome, to aggregate into microscopically visible specks due to its recruitment to the inflammasome. Methods to monitor this change in the spatial distribution of ASC in human peripheral blood monocytes by flow cytometry and fluorescence microscopy are presented.

AB - One of the most prominent effects of P2X7 activation in myeloid cells is the induction of the assembly of the NLRP3 inflammasome, a central process controlling the secretion of pro-inflammatory cytokines of the IL-1 family such as IL-1β and IL-18. The ability to visualize inflammasome formation greatly facilitates research into the role of P2X7 in inflammation. In this chapter, a method to monitor the formation of the NLPR3 inflammasome in monocytes and other myeloid cells could be demonstrated. Following priming by lipopolysaccharide (LPS), P2X7 was stimulated by ATP to mediate inflammasome assembly. This causes cytosolically disperse ASC, a central component of the inflammasome, to aggregate into microscopically visible specks due to its recruitment to the inflammasome. Methods to monitor this change in the spatial distribution of ASC in human peripheral blood monocytes by flow cytometry and fluorescence microscopy are presented.

KW - Flow Cytometry

KW - Humans

KW - Inflammasomes

KW - Lipopolysaccharides/pharmacology

KW - Microscopy, Fluorescence

KW - Monocytes

U2 - 10.1007/978-1-0716-2384-8_14

DO - 10.1007/978-1-0716-2384-8_14

M3 - SCORING: Contribution to collected editions/anthologies

C2 - 35776330

SN - 978-1-0716-2383-1

T3 - Methods in Molecular Biology

SP - 265

EP - 278

BT - The P2X7 Receptor

A2 - Nicke, Annette

PB - HUMANA PRESS INC

CY - New York, NY

ER -