PortalPublikationenVirally infected mouse liver endothelial cells trigger CD8+ ...

Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity

Standard

Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity. / Kern, Michaela; Popov, Alexey; Scholz, Kai; Schumak, Beatrix; Djandji, Dominik; Limmer, Andreas; Eggle, Daniela; Sacher, Torsten; Zawatzky, Rainer; Holtappels, Rafaela; Reddehase, Matthias J; Hartmann, Gunther; Debey-Pascher, Svenja; Diehl, Linda; Kalinke, Ulrich; Koszinowski, Ulrich; Schultze, Joachim; Knolle, Percy A.

in: GASTROENTEROLOGY, Jahrgang 138, Nr. 1, 01.2010, S. 336-46.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kern, M, Popov, A, Scholz, K, Schumak, B, Djandji, D, Limmer, A, Eggle, D, Sacher, T, Zawatzky, R, Holtappels, R, Reddehase, MJ, Hartmann, G, Debey-Pascher, S, Diehl, L, Kalinke, U, Koszinowski, U, Schultze, J & Knolle, PA 2010, 'Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity', GASTROENTEROLOGY, Jg. 138, Nr. 1, S. 336-46. https://doi.org/10.1053/j.gastro.2009.08.057

APA

Kern, M., Popov, A., Scholz, K., Schumak, B., Djandji, D., Limmer, A., Eggle, D., Sacher, T., Zawatzky, R., Holtappels, R., Reddehase, M. J., Hartmann, G., Debey-Pascher, S., Diehl, L., Kalinke, U., Koszinowski, U., Schultze, J., & Knolle, P. A. (2010). Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity. GASTROENTEROLOGY, 138(1), 336-46. https://doi.org/10.1053/j.gastro.2009.08.057

Vancouver

Kern M, Popov A, Scholz K, Schumak B, Djandji D, Limmer A et al. Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity. GASTROENTEROLOGY. 2010 Jan;138(1):336-46. https://doi.org/10.1053/j.gastro.2009.08.057

Bibtex

@article{89943ef6f8284e87ab7cb31422c1277b,
title = "Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity",
abstract = "BACKGROUND & AIMS: Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8(+) T-cell immunity through increased delivery of costimulatory signals instead of tolerance. Here we investigate whether organ-resident antigen-presenting cells, such as liver sinusoidal endothelial cells (LSECs), also switch from tolerogenic to immunogenic CD8(+) T-cell activation upon such stimulation.METHODS: Murine LSECs were isolated by immunomagnetic separation and analyzed for functional maturation upon triggering pattern recognition receptors or viral infection employing gene expression analysis and T cell coculture assays. In vivo relevance of the findings was confirmed with bone-marrow chimeric animals.RESULTS: LSECs expressed numerous pattern recognition receptors that allowed for sentinel function, but ligand-induced activation of these receptors was not sufficient to overcome tolerance induction of CD8(+) T cells. Importantly, viral infection with murine cytomegalovirus caused functional maturation of antigen-presenting LSECs and was sufficient to promote antigen-specific differentiation into effector CD8(+) T cells in the absence of dendritic cells and independent of CD80/86.CONCLUSIONS: These results shed new light on the generation of organ-specific immunity and may contribute to overcoming tolerance in relevant situations, such as cancer.",
keywords = "Adoptive Transfer, Animals, Bone Marrow, CD8-Positive T-Lymphocytes, Cell Differentiation, Cells, Cultured, Chimera, Endothelial Cells, Herpesviridae Infections, Immune Tolerance, Ligands, Liver, Mice, Muromegalovirus, Oligonucleotide Array Sequence Analysis, Organ Specificity, Receptors, Pattern Recognition",
author = "Michaela Kern and Alexey Popov and Kai Scholz and Beatrix Schumak and Dominik Djandji and Andreas Limmer and Daniela Eggle and Torsten Sacher and Rainer Zawatzky and Rafaela Holtappels and Reddehase, {Matthias J} and Gunther Hartmann and Svenja Debey-Pascher and Linda Diehl and Ulrich Kalinke and Ulrich Koszinowski and Joachim Schultze and Knolle, {Percy A}",
note = "Copyright 2010 AGA Institute. Published by Elsevier Inc. All rights reserved.",
year = "2010",
month = jan,
doi = "10.1053/j.gastro.2009.08.057",
language = "English",
volume = "138",
pages = "336--46",
journal = "GASTROENTEROLOGY",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Virally infected mouse liver endothelial cells trigger CD8+ T-cell immunity

AU - Kern, Michaela

AU - Popov, Alexey

AU - Scholz, Kai

AU - Schumak, Beatrix

AU - Djandji, Dominik

AU - Limmer, Andreas

AU - Eggle, Daniela

AU - Sacher, Torsten

AU - Zawatzky, Rainer

AU - Holtappels, Rafaela

AU - Reddehase, Matthias J

AU - Hartmann, Gunther

AU - Debey-Pascher, Svenja

AU - Diehl, Linda

AU - Kalinke, Ulrich

AU - Koszinowski, Ulrich

AU - Schultze, Joachim

AU - Knolle, Percy A

N1 - Copyright 2010 AGA Institute. Published by Elsevier Inc. All rights reserved.

PY - 2010/1

Y1 - 2010/1

N2 - BACKGROUND & AIMS: Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8(+) T-cell immunity through increased delivery of costimulatory signals instead of tolerance. Here we investigate whether organ-resident antigen-presenting cells, such as liver sinusoidal endothelial cells (LSECs), also switch from tolerogenic to immunogenic CD8(+) T-cell activation upon such stimulation.METHODS: Murine LSECs were isolated by immunomagnetic separation and analyzed for functional maturation upon triggering pattern recognition receptors or viral infection employing gene expression analysis and T cell coculture assays. In vivo relevance of the findings was confirmed with bone-marrow chimeric animals.RESULTS: LSECs expressed numerous pattern recognition receptors that allowed for sentinel function, but ligand-induced activation of these receptors was not sufficient to overcome tolerance induction of CD8(+) T cells. Importantly, viral infection with murine cytomegalovirus caused functional maturation of antigen-presenting LSECs and was sufficient to promote antigen-specific differentiation into effector CD8(+) T cells in the absence of dendritic cells and independent of CD80/86.CONCLUSIONS: These results shed new light on the generation of organ-specific immunity and may contribute to overcoming tolerance in relevant situations, such as cancer.

AB - BACKGROUND & AIMS: Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8(+) T-cell immunity through increased delivery of costimulatory signals instead of tolerance. Here we investigate whether organ-resident antigen-presenting cells, such as liver sinusoidal endothelial cells (LSECs), also switch from tolerogenic to immunogenic CD8(+) T-cell activation upon such stimulation.METHODS: Murine LSECs were isolated by immunomagnetic separation and analyzed for functional maturation upon triggering pattern recognition receptors or viral infection employing gene expression analysis and T cell coculture assays. In vivo relevance of the findings was confirmed with bone-marrow chimeric animals.RESULTS: LSECs expressed numerous pattern recognition receptors that allowed for sentinel function, but ligand-induced activation of these receptors was not sufficient to overcome tolerance induction of CD8(+) T cells. Importantly, viral infection with murine cytomegalovirus caused functional maturation of antigen-presenting LSECs and was sufficient to promote antigen-specific differentiation into effector CD8(+) T cells in the absence of dendritic cells and independent of CD80/86.CONCLUSIONS: These results shed new light on the generation of organ-specific immunity and may contribute to overcoming tolerance in relevant situations, such as cancer.

KW - Adoptive Transfer

KW - Animals

KW - Bone Marrow

KW - CD8-Positive T-Lymphocytes

KW - Cell Differentiation

KW - Cells, Cultured

KW - Chimera

KW - Endothelial Cells

KW - Herpesviridae Infections

KW - Immune Tolerance

KW - Ligands

KW - Liver

KW - Mice

KW - Muromegalovirus

KW - Oligonucleotide Array Sequence Analysis

KW - Organ Specificity

KW - Receptors, Pattern Recognition

U2 - 10.1053/j.gastro.2009.08.057

DO - 10.1053/j.gastro.2009.08.057

M3 - SCORING: Journal article

C2 - 19737567

VL - 138

SP - 336

EP - 346

JO - GASTROENTEROLOGY

JF - GASTROENTEROLOGY

SN - 0016-5085

IS - 1

ER -