Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants

TY - JOUR

T1 - Venomics of the Australian eastern brown snake (Pseudonaja textilis): Detection of new venom proteins and splicing variants

AU - Viala, Vincent Louis

AU - Hildebrand, Diana

AU - Trusch, Maria

AU - Fucase, Tamara Mieco

AU - Sciani, Juliana Mozer

AU - Pimenta, Daniel Carvalho

AU - Arni, Raghuvir K

AU - Schlüter, Hartmut

AU - Betzel, Christian

AU - Mirtschin, Peter

AU - Dunstan, Nathan

AU - Spencer, Patrick Jack

N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The eastern brown snake is the predominant cause of snakebites in mainland Australia. Its venom induces defibrination coagulopathy, renal failure and microangiopathic hemolytic anemia. Cardiovascular collapse has been described as an early cause of death in patients, but, so far, the mechanisms involved have not been fully identified. In the present work, we analysed the venome of Pseudonaja textilis by combining high throughput proteomics and transcriptomics, aiming to further characterize the components of this venom. The combination of these techniques in the analysis and identification of toxins, venom proteins and putative toxins allowed the sequence description and the identification of the following: prothrombinase coagulation factors, neurotoxic textilotoxin phospholipase A2 (PLA2) subunits and "acidic PLA2", three-finger toxins (3FTx) and the Kunitz-type protease inhibitor textilinin, venom metalloproteinase, C-type lectins, cysteine rich secretory proteins, calreticulin, dipeptidase 2, as well as evidences of Heloderma lizard peptides. Deep data-mining analysis revealed the secretion of a new transcript variant of venom coagulation factor 5a and the existence of a splicing variant of PLA2 modifying the UTR and signal peptide from a same mature protein. The transcriptome revealed the diversity of transcripts and mutations, and also indicates that splicing variants can be an important source of toxin variation.

AB - The eastern brown snake is the predominant cause of snakebites in mainland Australia. Its venom induces defibrination coagulopathy, renal failure and microangiopathic hemolytic anemia. Cardiovascular collapse has been described as an early cause of death in patients, but, so far, the mechanisms involved have not been fully identified. In the present work, we analysed the venome of Pseudonaja textilis by combining high throughput proteomics and transcriptomics, aiming to further characterize the components of this venom. The combination of these techniques in the analysis and identification of toxins, venom proteins and putative toxins allowed the sequence description and the identification of the following: prothrombinase coagulation factors, neurotoxic textilotoxin phospholipase A2 (PLA2) subunits and "acidic PLA2", three-finger toxins (3FTx) and the Kunitz-type protease inhibitor textilinin, venom metalloproteinase, C-type lectins, cysteine rich secretory proteins, calreticulin, dipeptidase 2, as well as evidences of Heloderma lizard peptides. Deep data-mining analysis revealed the secretion of a new transcript variant of venom coagulation factor 5a and the existence of a splicing variant of PLA2 modifying the UTR and signal peptide from a same mature protein. The transcriptome revealed the diversity of transcripts and mutations, and also indicates that splicing variants can be an important source of toxin variation.

U2 - 10.1016/j.toxicon.2015.06.005

DO - 10.1016/j.toxicon.2015.06.005

M3 - SCORING: Journal article

C2 - 26079951

VL - 107

SP - 252

EP - 265

JO - TOXICON

JF - TOXICON

SN - 0041-0101

IS - Pt B

ER -