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Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg

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Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg. / Khuseyinova, Natalie; Greven, Sonja; Rückerl, Regina; Trischler, Gerlinde; Loewel, Hannelore; Peters, Annette; Koenig, Wolfgang.

in: CLIN CHEM, Jahrgang 54, Nr. 1, 01.2008, S. 124-130.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung

Harvard

Khuseyinova, N, Greven, S, Rückerl, R, Trischler, G, Loewel, H, Peters, A & Koenig, W 2008, 'Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg', CLIN CHEM, Jg. 54, Nr. 1, S. 124-130. https://doi.org/10.1373/clinchem.2007.093468

APA

Khuseyinova, N., Greven, S., Rückerl, R., Trischler, G., Loewel, H., Peters, A., & Koenig, W. (2008). Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg. CLIN CHEM, 54(1), 124-130. https://doi.org/10.1373/clinchem.2007.093468

Vancouver

Khuseyinova N, Greven S, Rückerl R, Trischler G, Loewel H, Peters A et al. Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg. CLIN CHEM. 2008 Jan;54(1):124-130. https://doi.org/10.1373/clinchem.2007.093468

Bibtex

@article{7bb101c3345d427c826f4075fc7d8a3c,
title = "Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg",
abstract = "BACKGROUND: Of the numerous emerging biomarkers for coronary heart disease (CHD), lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), an enzyme involved in lipid metabolism and inflammatory pathways, seems to be a promising candidate. Implementation of Lp-PLA(2) measurement into clinical practice, however, requires data on the reliability of such measurements.METHODS: We measured Lp-PLA(2) concentrations by ELISA in blood samples drawn from 200 post-myocardial infarction patients (39-76 years) at 6 monthly intervals between May 2003 and February 2004, for a total of 1143 samples. We estimated analytical, within-individual, and between-individual variation, the critical difference, and the intraclass correlation coefficient of reliability (ICC) to assess the reliability of serial Lp-PLA(2) measurements.RESULTS: The mean (SD) plasma Lp-PLA(2) concentration for the study participants was 188.7 (41.8) microg/L, with no significant difference between men and women. The analytical CV for Lp-PLA(2) was 4.4%, the within-individual biological CV was 15%, and the between-individual CV was 22%. The ICC was 0.66. An important part of the total variation in plasma Lp-PLA(2) concentration was explained by the between-individual variation (as a percentage of the total variance, 66.1%), whereas the within-individual variance was 31.3%. The analytical variance was as low as 2.6%.CONCLUSIONS: Between-individual variation in Lp-PLA(2) concentration was substantially greater than within-individual variation. In general, our data demonstrate considerable stability and good reproducibility of serial Lp-PLA(2) measurements, results that compared favorably with those for the more commonly measured lipid markers.",
keywords = "1-Alkyl-2-acetylglycerophosphocholine Esterase/biosynthesis, Adult, Aged, Case-Control Studies, Cohort Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Myocardial Infarction/enzymology, Reproducibility of Results",
author = "Natalie Khuseyinova and Sonja Greven and Regina R{\"u}ckerl and Gerlinde Trischler and Hannelore Loewel and Annette Peters and Wolfgang Koenig",
year = "2008",
month = jan,
doi = "10.1373/clinchem.2007.093468",
language = "English",
volume = "54",
pages = "124--130",
journal = "CLIN CHEM",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Variability of serial lipoprotein-associated phospholipase A2 measurements in post myocardial infarction patients: results from the AIRGENE Study Center Augsburg

AU - Khuseyinova, Natalie

AU - Greven, Sonja

AU - Rückerl, Regina

AU - Trischler, Gerlinde

AU - Loewel, Hannelore

AU - Peters, Annette

AU - Koenig, Wolfgang

PY - 2008/1

Y1 - 2008/1

N2 - BACKGROUND: Of the numerous emerging biomarkers for coronary heart disease (CHD), lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), an enzyme involved in lipid metabolism and inflammatory pathways, seems to be a promising candidate. Implementation of Lp-PLA(2) measurement into clinical practice, however, requires data on the reliability of such measurements.METHODS: We measured Lp-PLA(2) concentrations by ELISA in blood samples drawn from 200 post-myocardial infarction patients (39-76 years) at 6 monthly intervals between May 2003 and February 2004, for a total of 1143 samples. We estimated analytical, within-individual, and between-individual variation, the critical difference, and the intraclass correlation coefficient of reliability (ICC) to assess the reliability of serial Lp-PLA(2) measurements.RESULTS: The mean (SD) plasma Lp-PLA(2) concentration for the study participants was 188.7 (41.8) microg/L, with no significant difference between men and women. The analytical CV for Lp-PLA(2) was 4.4%, the within-individual biological CV was 15%, and the between-individual CV was 22%. The ICC was 0.66. An important part of the total variation in plasma Lp-PLA(2) concentration was explained by the between-individual variation (as a percentage of the total variance, 66.1%), whereas the within-individual variance was 31.3%. The analytical variance was as low as 2.6%.CONCLUSIONS: Between-individual variation in Lp-PLA(2) concentration was substantially greater than within-individual variation. In general, our data demonstrate considerable stability and good reproducibility of serial Lp-PLA(2) measurements, results that compared favorably with those for the more commonly measured lipid markers.

AB - BACKGROUND: Of the numerous emerging biomarkers for coronary heart disease (CHD), lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), an enzyme involved in lipid metabolism and inflammatory pathways, seems to be a promising candidate. Implementation of Lp-PLA(2) measurement into clinical practice, however, requires data on the reliability of such measurements.METHODS: We measured Lp-PLA(2) concentrations by ELISA in blood samples drawn from 200 post-myocardial infarction patients (39-76 years) at 6 monthly intervals between May 2003 and February 2004, for a total of 1143 samples. We estimated analytical, within-individual, and between-individual variation, the critical difference, and the intraclass correlation coefficient of reliability (ICC) to assess the reliability of serial Lp-PLA(2) measurements.RESULTS: The mean (SD) plasma Lp-PLA(2) concentration for the study participants was 188.7 (41.8) microg/L, with no significant difference between men and women. The analytical CV for Lp-PLA(2) was 4.4%, the within-individual biological CV was 15%, and the between-individual CV was 22%. The ICC was 0.66. An important part of the total variation in plasma Lp-PLA(2) concentration was explained by the between-individual variation (as a percentage of the total variance, 66.1%), whereas the within-individual variance was 31.3%. The analytical variance was as low as 2.6%.CONCLUSIONS: Between-individual variation in Lp-PLA(2) concentration was substantially greater than within-individual variation. In general, our data demonstrate considerable stability and good reproducibility of serial Lp-PLA(2) measurements, results that compared favorably with those for the more commonly measured lipid markers.

KW - 1-Alkyl-2-acetylglycerophosphocholine Esterase/biosynthesis

KW - Adult

KW - Aged

KW - Case-Control Studies

KW - Cohort Studies

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Myocardial Infarction/enzymology

KW - Reproducibility of Results

U2 - 10.1373/clinchem.2007.093468

DO - 10.1373/clinchem.2007.093468

M3 - SCORING: Journal article

C2 - 18024527

VL - 54

SP - 124

EP - 130

JO - CLIN CHEM

JF - CLIN CHEM

SN - 0009-9147

IS - 1

ER -