Validation of VX2 as a Hepatocellular Carcinoma Model: Comparison of the Molecular Reaction of VX2 and HepG2 Tumor Cells to Sorafenib In Vitro
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Validation of VX2 as a Hepatocellular Carcinoma Model: Comparison of the Molecular Reaction of VX2 and HepG2 Tumor Cells to Sorafenib In Vitro. / Nass, Norbert; Streit, Sebastian; Wybranski, Christian; Jürgens, Julian; Brauner, Jan; Schulz, Nadine; Powerski, Maciej; Ricke, Jens; Kalinski, Thomas; Dudeck, Oliver; Seidensticker, Max.
in: ANTICANCER RES, Jahrgang 37, Nr. 1, 01.2017, S. 87-93.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Validation of VX2 as a Hepatocellular Carcinoma Model: Comparison of the Molecular Reaction of VX2 and HepG2 Tumor Cells to Sorafenib In Vitro
AU - Nass, Norbert
AU - Streit, Sebastian
AU - Wybranski, Christian
AU - Jürgens, Julian
AU - Brauner, Jan
AU - Schulz, Nadine
AU - Powerski, Maciej
AU - Ricke, Jens
AU - Kalinski, Thomas
AU - Dudeck, Oliver
AU - Seidensticker, Max
N1 - Copyright© 2017 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
PY - 2017/1
Y1 - 2017/1
N2 - As there is currently no superior hepatocellular carcinoma (HCC) model with percutaneous vascular access for transarterial treatments available, the VX2 rabbit model is frequently used for in vivo investigations on liver carcinoma. However, the VX2 cell line was derived from a virus-induced skin papilloma that can form carcinosarcoma in liver of rabbits and the transferability of obtained results to HCC treatment remains open. Here we compared the most frequently investigated human HCC model cell line, HepG2, with VX2 cells in vitro in terms of sensitivity towards the broad specificity kinase inhibitor sorafenib and responsiveness to the addition of platelet-derived growth factor AB (PDGF-AB), vascular endothelial growth factor (VEGF) and hepatic growth factor (HGF), as well as insulin and interleukin-1β (IL1β). Phosphorylation of protein kinase B (AKT) the mitogen-activated protein kinases (MAPKs) p38 and p42/44 (extracellular signal-regulated kinase, ERK1/2) and inhibitor of kappa light chain gene enhancer alpha (IĸBα) was determined by western blotting as these events are associated with early signaling cascades. Additionally, the inhibition of phosphorylation under sorafenib treatment was investigated. Sorafenib was equally toxic to both cell lines, but only in HepG2 was activation of caspase 3/7 activity, as a sign of apoptosis, observed. VX2 cells exhibited generally more intense phosphorylation signals in response to the growth factors and also serum. In contrast to VX2, HepG2 cells showed no response to PDGF-AB or VEGF as determined by kinase phosphorylation. In both cell lines, sorafenib inhibited growth factor-induced phosphorylation of ERK and p38-MAPK. AKT phosphorylation was only inhibited in VX2 cells and IĸBα phosphorylation was not influenced by this kinase inhibitor in either cell type. Taken together, the two cellular models for HCC share several features related to sorafenib application, but differed in their responsiveness towards growth factors. Therefore, results obtained with the VX2 model cannot be extended to human HCC without appropriate caution.
AB - As there is currently no superior hepatocellular carcinoma (HCC) model with percutaneous vascular access for transarterial treatments available, the VX2 rabbit model is frequently used for in vivo investigations on liver carcinoma. However, the VX2 cell line was derived from a virus-induced skin papilloma that can form carcinosarcoma in liver of rabbits and the transferability of obtained results to HCC treatment remains open. Here we compared the most frequently investigated human HCC model cell line, HepG2, with VX2 cells in vitro in terms of sensitivity towards the broad specificity kinase inhibitor sorafenib and responsiveness to the addition of platelet-derived growth factor AB (PDGF-AB), vascular endothelial growth factor (VEGF) and hepatic growth factor (HGF), as well as insulin and interleukin-1β (IL1β). Phosphorylation of protein kinase B (AKT) the mitogen-activated protein kinases (MAPKs) p38 and p42/44 (extracellular signal-regulated kinase, ERK1/2) and inhibitor of kappa light chain gene enhancer alpha (IĸBα) was determined by western blotting as these events are associated with early signaling cascades. Additionally, the inhibition of phosphorylation under sorafenib treatment was investigated. Sorafenib was equally toxic to both cell lines, but only in HepG2 was activation of caspase 3/7 activity, as a sign of apoptosis, observed. VX2 cells exhibited generally more intense phosphorylation signals in response to the growth factors and also serum. In contrast to VX2, HepG2 cells showed no response to PDGF-AB or VEGF as determined by kinase phosphorylation. In both cell lines, sorafenib inhibited growth factor-induced phosphorylation of ERK and p38-MAPK. AKT phosphorylation was only inhibited in VX2 cells and IĸBα phosphorylation was not influenced by this kinase inhibitor in either cell type. Taken together, the two cellular models for HCC share several features related to sorafenib application, but differed in their responsiveness towards growth factors. Therefore, results obtained with the VX2 model cannot be extended to human HCC without appropriate caution.
KW - Animals
KW - Antineoplastic Agents
KW - Apoptosis
KW - Carcinoma, Hepatocellular
KW - Cell Proliferation
KW - Cell Survival
KW - Dose-Response Relationship, Drug
KW - Hep G2 Cells
KW - Hepatocyte Growth Factor
KW - Humans
KW - Insulin
KW - Interleukin-1beta
KW - Liver Neoplasms
KW - Niacinamide
KW - Phenotype
KW - Phenylurea Compounds
KW - Phosphorylation
KW - Platelet-Derived Growth Factor
KW - Protein Kinase Inhibitors
KW - Protein-Tyrosine Kinases
KW - Rabbits
KW - Signal Transduction
KW - Time Factors
KW - Vascular Endothelial Growth Factor A
KW - Comparative Study
KW - Journal Article
KW - Validation Studies
U2 - 10.21873/anticanres.11293
DO - 10.21873/anticanres.11293
M3 - SCORING: Journal article
C2 - 28011478
VL - 37
SP - 87
EP - 93
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 1
ER -