Validation of cord blood split products prepared by an automated method.

TY - JOUR

T1 - Validation of cord blood split products prepared by an automated method.

AU - Gutensohn, K

AU - Odendahl, M

AU - Kersten, J F

AU - Tonn, T

N1 - © 2012 The Authors. Transfusion Medicine © 2012 British Blood Transfusion Society.

PY - 2013

Y1 - 2013

N2 - OBJECTIVES: In this study, we studied whether the contents of the two compartments of automatically processed cord blood (CB) units are comparable with respect to cell counts and viability and therefore suitable for clinical therapy.BACKGROUND: CB-derived stem cells are increasingly used for allogeneic transplantation. Many centres prepare the transplants by automated methods allowing to split the product into two portions.METHODS: CB was collected at different sites in Germany and transported to a single centre for processing. Before and after cryopreservation laboratory analyses were performed to compare the quality of the two CB segments.RESULTS: In total, 33 products were processed [mean collection volume: 18·6 ± 1·2 mL (range 15·2-20·2 mL) segment A; mean: 4·7 ± 0·3 mL (range 4·2-5·2 mL) segment B]. CD34+ cell counts, viability of CD34+ cells and many other haematological parameters showed a good comparibility between the two segments. However, lymphocyte counts and results of clonogenic assays were significantly different between the two segments of the split product.CONCLUSION: We conclude that the preparation of the cord blood unit by the automated process results in a homogenous distribution of stem and progenitor cells. However, our findings show that the clonogenic capacity differs between the two segments.

AB - OBJECTIVES: In this study, we studied whether the contents of the two compartments of automatically processed cord blood (CB) units are comparable with respect to cell counts and viability and therefore suitable for clinical therapy.BACKGROUND: CB-derived stem cells are increasingly used for allogeneic transplantation. Many centres prepare the transplants by automated methods allowing to split the product into two portions.METHODS: CB was collected at different sites in Germany and transported to a single centre for processing. Before and after cryopreservation laboratory analyses were performed to compare the quality of the two CB segments.RESULTS: In total, 33 products were processed [mean collection volume: 18·6 ± 1·2 mL (range 15·2-20·2 mL) segment A; mean: 4·7 ± 0·3 mL (range 4·2-5·2 mL) segment B]. CD34+ cell counts, viability of CD34+ cells and many other haematological parameters showed a good comparibility between the two segments. However, lymphocyte counts and results of clonogenic assays were significantly different between the two segments of the split product.CONCLUSION: We conclude that the preparation of the cord blood unit by the automated process results in a homogenous distribution of stem and progenitor cells. However, our findings show that the clonogenic capacity differs between the two segments.

KW - Humans

KW - Infant, Newborn

KW - Flow Cytometry

KW - Cryopreservation

KW - Cell Survival

KW - Blood Cell Count

KW - Automation

KW - Colony-Forming Units Assay

KW - Antigens, CD34/analysis

KW - Blood Preservation

KW - Cell Separation/methods

KW - Centrifugation

KW - Cord Blood Stem Cell Transplantation

KW - Fetal Blood/cytology

KW - Humans

KW - Infant, Newborn

KW - Flow Cytometry

KW - Cryopreservation

KW - Cell Survival

KW - Blood Cell Count

KW - Automation

KW - Colony-Forming Units Assay

KW - Antigens, CD34/analysis

KW - Blood Preservation

KW - Cell Separation/methods

KW - Centrifugation

KW - Cord Blood Stem Cell Transplantation

KW - Fetal Blood/cytology

U2 - 10.1111/j.1365-3148.2012.01191.x

DO - 10.1111/j.1365-3148.2012.01191.x

M3 - SCORING: Journal article

C2 - 23025789

VL - 23

SP - 48

EP - 54

JO - TRANSFUSION MED

JF - TRANSFUSION MED

SN - 0958-7578

IS - 1

M1 - 1

ER -