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Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels

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Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels. / Kaschubowski, Klaus E; Kraft, Axel E; Nikolaev, Viacheslav O; Haag, Friedrich.

in: Methods Mol Biol, Jahrgang 2041, 2020, S. 223-231.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kaschubowski, KE, Kraft, AE, Nikolaev, VO & Haag, F 2020, 'Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels', Methods Mol Biol, Jg. 2041, S. 223-231. https://doi.org/10.1007/978-1-4939-9717-6_16

APA

Kaschubowski, K. E., Kraft, A. E., Nikolaev, V. O., & Haag, F. (2020). Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels. Methods Mol Biol, 2041, 223-231. https://doi.org/10.1007/978-1-4939-9717-6_16

Vancouver

Kaschubowski KE, Kraft AE, Nikolaev VO, Haag F. Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels. Methods Mol Biol. 2020;2041:223-231. https://doi.org/10.1007/978-1-4939-9717-6_16

Bibtex

@article{18b1397eb8ce42e48a38dc57190fb5d7,
title = "Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels",
abstract = "The assessment of local concentrations of extracellular ATP (eATP) at the site of receptor binding remains a challenge in the field of purinergic signaling. In many cases, biosensors exploiting the principle of F{\"o}rster resonance energy transfer (FRET) have provided useful tools to visualize local concentrations of metabolites. A series of FRET-based biosensors based on the epsilon subunits of bacterial ATP synthases have been described for the visualisation of ATP. These sensors carry ATP-sensing units with different affinities for ATP, permitting imaging of ATP under the widely different concentration conditions found in subcellular locations such as the cytoplasm and the membrane-proximal extracellular space.",
author = "Kaschubowski, {Klaus E} and Kraft, {Axel E} and Nikolaev, {Viacheslav O} and Friedrich Haag",
year = "2020",
doi = "10.1007/978-1-4939-9717-6_16",
language = "English",
volume = "2041",
pages = "223--231",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels

AU - Kaschubowski, Klaus E

AU - Kraft, Axel E

AU - Nikolaev, Viacheslav O

AU - Haag, Friedrich

PY - 2020

Y1 - 2020

N2 - The assessment of local concentrations of extracellular ATP (eATP) at the site of receptor binding remains a challenge in the field of purinergic signaling. In many cases, biosensors exploiting the principle of Förster resonance energy transfer (FRET) have provided useful tools to visualize local concentrations of metabolites. A series of FRET-based biosensors based on the epsilon subunits of bacterial ATP synthases have been described for the visualisation of ATP. These sensors carry ATP-sensing units with different affinities for ATP, permitting imaging of ATP under the widely different concentration conditions found in subcellular locations such as the cytoplasm and the membrane-proximal extracellular space.

AB - The assessment of local concentrations of extracellular ATP (eATP) at the site of receptor binding remains a challenge in the field of purinergic signaling. In many cases, biosensors exploiting the principle of Förster resonance energy transfer (FRET) have provided useful tools to visualize local concentrations of metabolites. A series of FRET-based biosensors based on the epsilon subunits of bacterial ATP synthases have been described for the visualisation of ATP. These sensors carry ATP-sensing units with different affinities for ATP, permitting imaging of ATP under the widely different concentration conditions found in subcellular locations such as the cytoplasm and the membrane-proximal extracellular space.

U2 - 10.1007/978-1-4939-9717-6_16

DO - 10.1007/978-1-4939-9717-6_16

M3 - SCORING: Journal article

C2 - 31646492

VL - 2041

SP - 223

EP - 231

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -