Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
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Urinary alpha1-antichymotrypsin: a biomarker of prion infection. / Miele, Gino; Seeger, Harald; Marino, Denis; Eberhard, Ralf; Heikenwalder, Mathias; Stoeck, Katharina; Basagni, Max; Knight, Richard; Green, Alison; Chianini, Francesca; Wüthrich, Rudolf P; Hock, Christoph; Zerr, Inga; Aguzzi, Adriano.
in: PLOS ONE, Jahrgang 3, Nr. 12, 12, 2008, S. 3870.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Urinary alpha1-antichymotrypsin: a biomarker of prion infection.
AU - Miele, Gino
AU - Seeger, Harald
AU - Marino, Denis
AU - Eberhard, Ralf
AU - Heikenwalder, Mathias
AU - Stoeck, Katharina
AU - Basagni, Max
AU - Knight, Richard
AU - Green, Alison
AU - Chianini, Francesca
AU - Wüthrich, Rudolf P
AU - Hock, Christoph
AU - Zerr, Inga
AU - Aguzzi, Adriano
PY - 2008
Y1 - 2008
N2 - The occurrence of blood-borne prion transmission incidents calls for identification of potential prion carriers. However, current methods for intravital diagnosis of prion disease rely on invasive tissue biopsies and are unsuitable for large-scale screening. Sensitive biomarkers may help meeting this need. Here we scanned the genome for transcripts elevated upon prion infection and encoding secreted proteins. We found that alpha(1)-antichymotrypsin (alpha(1)-ACT) was highly upregulated in brains of scrapie-infected mice. Furthermore, alpha(1)-ACT levels were dramatically increased in urine of patients suffering from sporadic Creutzfeldt-Jakob disease, and increased progressively throughout the disease. Increased alpha(1)-ACT excretion was also found in cases of natural prion disease of animals. Therefore measurement of urinary alpha(1)-ACT levels may be useful for monitoring the efficacy of therapeutic regimens for prion disease, and possibly also for deferring blood and organ donors that may be at risk of transmitting prion infections.
AB - The occurrence of blood-borne prion transmission incidents calls for identification of potential prion carriers. However, current methods for intravital diagnosis of prion disease rely on invasive tissue biopsies and are unsuitable for large-scale screening. Sensitive biomarkers may help meeting this need. Here we scanned the genome for transcripts elevated upon prion infection and encoding secreted proteins. We found that alpha(1)-antichymotrypsin (alpha(1)-ACT) was highly upregulated in brains of scrapie-infected mice. Furthermore, alpha(1)-ACT levels were dramatically increased in urine of patients suffering from sporadic Creutzfeldt-Jakob disease, and increased progressively throughout the disease. Increased alpha(1)-ACT excretion was also found in cases of natural prion disease of animals. Therefore measurement of urinary alpha(1)-ACT levels may be useful for monitoring the efficacy of therapeutic regimens for prion disease, and possibly also for deferring blood and organ donors that may be at risk of transmitting prion infections.
M3 - SCORING: Zeitschriftenaufsatz
VL - 3
SP - 3870
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 12
M1 - 12
ER -
