Upregulation of cathepsin S in psoriatic keratinocytes.
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Upregulation of cathepsin S in psoriatic keratinocytes. / Schönefuss, Alexander; Wendt, Wiebke; Schattling, Benjamin; Schulten, Roxane; Hoffmann, Klaus; Stuecker, Markus; Tigges, Christian; Lübbert, Hermann; Stichel, Christine.
in: EXP DERMATOL, Jahrgang 19, Nr. 8, 8, 2010, S. 80-88.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Upregulation of cathepsin S in psoriatic keratinocytes.
AU - Schönefuss, Alexander
AU - Wendt, Wiebke
AU - Schattling, Benjamin
AU - Schulten, Roxane
AU - Hoffmann, Klaus
AU - Stuecker, Markus
AU - Tigges, Christian
AU - Lübbert, Hermann
AU - Stichel, Christine
PY - 2010
Y1 - 2010
N2 - Cathepsin S (CATS) is a cysteine protease, well known for its role in MHC class II-mediated antigen presentation and extracellular matrix degradation. Disturbance of the expression or metabolism of this protease is a concomitant feature of several diseases. Given this importance we studied the localization and regulation of CATS expression in normal and pathological human/mouse skin. In normal human skin CATS-immunostaining is mainly present in the dermis and is localized in macrophages, Langerhans, T- and endothelial cells, but absent in keratinocytes. In all analyzed pathological skin biopsies, i.e. atopic dermatitis, actinic keratosis and psoriasis, CATS staining is strongly increased in the dermis. But only in psoriasis, CATS-immunostaining is also detectable in keratinocytes. We show that cocultivation with T-cells as well as treatment with cytokines can trigger expression and secretion of CATS, which is involved in MHC II processing in keratinocytes. Our data provide first evidence that CATS expression (i) is selectively induced in psoriatic keratinocytes, (ii) is triggered by T-cells and (iii) might be involved in keratinocytic MHC class II expression, the processing of the MHC class II-associated invariant chain and remodeling of the extracellular matrix. This paper expands our knowledge on the important role of keratinocytes in dermatological disease.
AB - Cathepsin S (CATS) is a cysteine protease, well known for its role in MHC class II-mediated antigen presentation and extracellular matrix degradation. Disturbance of the expression or metabolism of this protease is a concomitant feature of several diseases. Given this importance we studied the localization and regulation of CATS expression in normal and pathological human/mouse skin. In normal human skin CATS-immunostaining is mainly present in the dermis and is localized in macrophages, Langerhans, T- and endothelial cells, but absent in keratinocytes. In all analyzed pathological skin biopsies, i.e. atopic dermatitis, actinic keratosis and psoriasis, CATS staining is strongly increased in the dermis. But only in psoriasis, CATS-immunostaining is also detectable in keratinocytes. We show that cocultivation with T-cells as well as treatment with cytokines can trigger expression and secretion of CATS, which is involved in MHC II processing in keratinocytes. Our data provide first evidence that CATS expression (i) is selectively induced in psoriatic keratinocytes, (ii) is triggered by T-cells and (iii) might be involved in keratinocytic MHC class II expression, the processing of the MHC class II-associated invariant chain and remodeling of the extracellular matrix. This paper expands our knowledge on the important role of keratinocytes in dermatological disease.
KW - Animals
KW - Biopsy
KW - Humans
KW - Disease Models, Animal
KW - Mice
KW - Coculture Techniques
KW - Cell Line
KW - Cathepsins metabolism
KW - Cell Communication
KW - Cytokines pharmacology
KW - Dermatitis, Atopic chemically induced
KW - Keratinocytes drug effects
KW - Major Histocompatibility Complex
KW - Oxazolone adverse effects
KW - Psoriasis metabolism
KW - T-Lymphocytes pathology
KW - Up-Regulation physiology
KW - Animals
KW - Biopsy
KW - Humans
KW - Disease Models, Animal
KW - Mice
KW - Coculture Techniques
KW - Cell Line
KW - Cathepsins metabolism
KW - Cell Communication
KW - Cytokines pharmacology
KW - Dermatitis, Atopic chemically induced
KW - Keratinocytes drug effects
KW - Major Histocompatibility Complex
KW - Oxazolone adverse effects
KW - Psoriasis metabolism
KW - T-Lymphocytes pathology
KW - Up-Regulation physiology
M3 - SCORING: Zeitschriftenaufsatz
VL - 19
SP - 80
EP - 88
JO - EXP DERMATOL
JF - EXP DERMATOL
SN - 0906-6705
IS - 8
M1 - 8
ER -
