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Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits

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Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits. / Prat-Vidal, C; Roura, S; Farré, J; Gálvez, C; Llach, A; Molina, C E; Hove-Madsen, L; Garcia, J; Cinca, J; Bayes-Genis, A.

in: TRANSPL P, Jahrgang 39, Nr. 7, 09.2007, S. 2434-7.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Prat-Vidal, C, Roura, S, Farré, J, Gálvez, C, Llach, A, Molina, CE, Hove-Madsen, L, Garcia, J, Cinca, J & Bayes-Genis, A 2007, 'Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits', TRANSPL P, Jg. 39, Nr. 7, S. 2434-7. https://doi.org/10.1016/j.transproceed.2007.06.016

APA

Prat-Vidal, C., Roura, S., Farré, J., Gálvez, C., Llach, A., Molina, C. E., Hove-Madsen, L., Garcia, J., Cinca, J., & Bayes-Genis, A. (2007). Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits. TRANSPL P, 39(7), 2434-7. https://doi.org/10.1016/j.transproceed.2007.06.016

Vancouver

Prat-Vidal C, Roura S, Farré J, Gálvez C, Llach A, Molina CE et al. Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits. TRANSPL P. 2007 Sep;39(7):2434-7. https://doi.org/10.1016/j.transproceed.2007.06.016

Bibtex

@article{8182494d19044108867bdd6e9d251518,
title = "Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits",
abstract = "BACKGROUND: Umbilical cord blood (UCB) has been widely used for hematopoietic stem cell transplantation. The UCB-derived stem cells (UCBSCs) have been proposed as an alternative to bone marrow (BM)-derived mesenchymal stem cells (MSCs) for cardiac cell-based therapy. Herein we studied whether UCBSCs spontaneously exhibit cardiac-specific markers in vitro.METHODS: Human UCBSCs were isolated, expanded, and phenotyped by flow cytometry, quantitative RT-PCR, and immunofluorescence. Cell pluripotency and proliferation were also assessed by adipogenic and osteogenic media and in growth assays.RESULTS: Among 25 analyzed UCB, 16% of cases afforded primary culture satisfactory generation of UCBSCs. Duplication time (Td) of cultures was 2.16 +/- 0.06 days. The cells were strongly positive for CD105 (18.5 +/- 0.14), CD44 (27 +/- 2.8), CD166 (13 +/- 9), CD29 (59 +/- 9.4), CD90 (60 +/- 11) and consistently negative for CD117 (1.2 +/- 0.1), CD106 (1.1 +/- 0), CD34 (1.2 +/- 0.2), CD14 (1 +/- 0), and CD45 (1 +/- 0), consistent with a mesenchymal lineage. Adipogenesis and osteogenesis of cells resulted in low accumulation of intracellular lipid droplets and high deposition of calcium. The UCBSCs showed gene transcripts for alpha-actinin, connexin (Cx)-43, SERCA-2, and stromal cell-derived factor (SDF)-1alpha. At the protein level, the cells abundantly expressed alpha-actinin, Cx-43, SERCA-2 and SDF-1alpha. In contrast, these cells did not express the cardiac transcription factors GATA-4, Tbx5, and Nkx2.5, nor the sarcomeric proteins beta-myosin heavy chain (beta-MyHC) or cardiac troponin I (cTnI).CONCLUSIONS: Human UCBSCs may represent an alternative source of stem cells for myocardial-cell replacement. These cells can be highly expanded. They spontaneously express proteins of paramount importance for cardiovascular regeneration, such as Cx-43, SERCA-2, and SDF-1alpha.",
keywords = "Adult, Bone Marrow Cells, Cell Culture Techniques, Cell Differentiation, Cord Blood Stem Cell Transplantation, Fetal Blood, Heart, Humans, Infant, Newborn, Mesoderm, Myocardium, Phenotype, Stem Cells, Treatment Outcome, Journal Article, Research Support, Non-U.S. Gov't",
author = "C Prat-Vidal and S Roura and J Farr{\'e} and C G{\'a}lvez and A Llach and Molina, {C E} and L Hove-Madsen and J Garcia and J Cinca and A Bayes-Genis",
year = "2007",
month = sep,
doi = "10.1016/j.transproceed.2007.06.016",
language = "English",
volume = "39",
pages = "2434--7",
journal = "TRANSPL P",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "7",

}

RIS

TY - JOUR

T1 - Umbilical cord blood-derived stem cells spontaneously express cardiomyogenic traits

AU - Prat-Vidal, C

AU - Roura, S

AU - Farré, J

AU - Gálvez, C

AU - Llach, A

AU - Molina, C E

AU - Hove-Madsen, L

AU - Garcia, J

AU - Cinca, J

AU - Bayes-Genis, A

PY - 2007/9

Y1 - 2007/9

N2 - BACKGROUND: Umbilical cord blood (UCB) has been widely used for hematopoietic stem cell transplantation. The UCB-derived stem cells (UCBSCs) have been proposed as an alternative to bone marrow (BM)-derived mesenchymal stem cells (MSCs) for cardiac cell-based therapy. Herein we studied whether UCBSCs spontaneously exhibit cardiac-specific markers in vitro.METHODS: Human UCBSCs were isolated, expanded, and phenotyped by flow cytometry, quantitative RT-PCR, and immunofluorescence. Cell pluripotency and proliferation were also assessed by adipogenic and osteogenic media and in growth assays.RESULTS: Among 25 analyzed UCB, 16% of cases afforded primary culture satisfactory generation of UCBSCs. Duplication time (Td) of cultures was 2.16 +/- 0.06 days. The cells were strongly positive for CD105 (18.5 +/- 0.14), CD44 (27 +/- 2.8), CD166 (13 +/- 9), CD29 (59 +/- 9.4), CD90 (60 +/- 11) and consistently negative for CD117 (1.2 +/- 0.1), CD106 (1.1 +/- 0), CD34 (1.2 +/- 0.2), CD14 (1 +/- 0), and CD45 (1 +/- 0), consistent with a mesenchymal lineage. Adipogenesis and osteogenesis of cells resulted in low accumulation of intracellular lipid droplets and high deposition of calcium. The UCBSCs showed gene transcripts for alpha-actinin, connexin (Cx)-43, SERCA-2, and stromal cell-derived factor (SDF)-1alpha. At the protein level, the cells abundantly expressed alpha-actinin, Cx-43, SERCA-2 and SDF-1alpha. In contrast, these cells did not express the cardiac transcription factors GATA-4, Tbx5, and Nkx2.5, nor the sarcomeric proteins beta-myosin heavy chain (beta-MyHC) or cardiac troponin I (cTnI).CONCLUSIONS: Human UCBSCs may represent an alternative source of stem cells for myocardial-cell replacement. These cells can be highly expanded. They spontaneously express proteins of paramount importance for cardiovascular regeneration, such as Cx-43, SERCA-2, and SDF-1alpha.

AB - BACKGROUND: Umbilical cord blood (UCB) has been widely used for hematopoietic stem cell transplantation. The UCB-derived stem cells (UCBSCs) have been proposed as an alternative to bone marrow (BM)-derived mesenchymal stem cells (MSCs) for cardiac cell-based therapy. Herein we studied whether UCBSCs spontaneously exhibit cardiac-specific markers in vitro.METHODS: Human UCBSCs were isolated, expanded, and phenotyped by flow cytometry, quantitative RT-PCR, and immunofluorescence. Cell pluripotency and proliferation were also assessed by adipogenic and osteogenic media and in growth assays.RESULTS: Among 25 analyzed UCB, 16% of cases afforded primary culture satisfactory generation of UCBSCs. Duplication time (Td) of cultures was 2.16 +/- 0.06 days. The cells were strongly positive for CD105 (18.5 +/- 0.14), CD44 (27 +/- 2.8), CD166 (13 +/- 9), CD29 (59 +/- 9.4), CD90 (60 +/- 11) and consistently negative for CD117 (1.2 +/- 0.1), CD106 (1.1 +/- 0), CD34 (1.2 +/- 0.2), CD14 (1 +/- 0), and CD45 (1 +/- 0), consistent with a mesenchymal lineage. Adipogenesis and osteogenesis of cells resulted in low accumulation of intracellular lipid droplets and high deposition of calcium. The UCBSCs showed gene transcripts for alpha-actinin, connexin (Cx)-43, SERCA-2, and stromal cell-derived factor (SDF)-1alpha. At the protein level, the cells abundantly expressed alpha-actinin, Cx-43, SERCA-2 and SDF-1alpha. In contrast, these cells did not express the cardiac transcription factors GATA-4, Tbx5, and Nkx2.5, nor the sarcomeric proteins beta-myosin heavy chain (beta-MyHC) or cardiac troponin I (cTnI).CONCLUSIONS: Human UCBSCs may represent an alternative source of stem cells for myocardial-cell replacement. These cells can be highly expanded. They spontaneously express proteins of paramount importance for cardiovascular regeneration, such as Cx-43, SERCA-2, and SDF-1alpha.

KW - Adult

KW - Bone Marrow Cells

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cord Blood Stem Cell Transplantation

KW - Fetal Blood

KW - Heart

KW - Humans

KW - Infant, Newborn

KW - Mesoderm

KW - Myocardium

KW - Phenotype

KW - Stem Cells

KW - Treatment Outcome

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.transproceed.2007.06.016

DO - 10.1016/j.transproceed.2007.06.016

M3 - SCORING: Journal article

C2 - 17889212

VL - 39

SP - 2434

EP - 2437

JO - TRANSPL P

JF - TRANSPL P

SN - 0041-1345

IS - 7

ER -