Tubular, lactating, and ductal adenomas are devoid of MED12 Exon2 mutations, and ductal adenomas show recurrent mutations in GNAS and the PI3K-AKT pathway
Standard
Tubular, lactating, and ductal adenomas are devoid of MED12 Exon2 mutations, and ductal adenomas show recurrent mutations in GNAS and the PI3K-AKT pathway. / Volckmar, Anna-Lena; Leichsenring, Jonas; Flechtenmacher, Christa; Pfarr, Nicole; Siebolts, Udo; Kirchner, Martina; Budczies, Jan; Bockmayr, Michael; Ridinger, Kathrin; Lorenz, Katja; Herpel, Esther; Noske, Aurelia; Weichert, Wilko; Klauschen, Frederick; Schirmacher, Peter; Penzel, Roland; Endris, Volker; Stenzinger, Albrecht.
in: GENE CHROMOSOME CANC, Jahrgang 56, Nr. 1, 01.2017, S. 11-17.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Tubular, lactating, and ductal adenomas are devoid of MED12 Exon2 mutations, and ductal adenomas show recurrent mutations in GNAS and the PI3K-AKT pathway
AU - Volckmar, Anna-Lena
AU - Leichsenring, Jonas
AU - Flechtenmacher, Christa
AU - Pfarr, Nicole
AU - Siebolts, Udo
AU - Kirchner, Martina
AU - Budczies, Jan
AU - Bockmayr, Michael
AU - Ridinger, Kathrin
AU - Lorenz, Katja
AU - Herpel, Esther
AU - Noske, Aurelia
AU - Weichert, Wilko
AU - Klauschen, Frederick
AU - Schirmacher, Peter
AU - Penzel, Roland
AU - Endris, Volker
AU - Stenzinger, Albrecht
N1 - © 2016 Wiley Periodicals, Inc.
PY - 2017/1
Y1 - 2017/1
N2 - Adenomas of the breast are rare benign tumors although single cases with malignant behavior have been reported. However, the genetic basis of these tumors is unknown. Employing targeted next generation sequencing of 50 cancer-related genes as well as Sanger sequencing, we profiled a cohort of 18 mammary adenomas comprising 9 ductal, 6 tubular, and 3 lactating adenoma. Missense mutations were detected in 8 of the 18 cases (44%). Specifically, five (56%) ductal adenomas and three (50%) tubular adenomas harbored mutated genes. No mutations were detected in lactating adenomas. Three of the nine ductal adenomas showed mutant AKT1 (p.E17K) with two of them harboring an additional GNAS mutation (p.R201C). One case had mutant PIK3CA (p.H1047R) and another case a mutation in GNAS (p.R201C). The three cases of mutated tubular adenomas showed mutations in either MET or FGFR3. Of note, we did not detect copy number changes and none of the cases including tubular adenomas had mutations in exon 2 of MED12. Our results suggest that ductal adenomas are related to papillomas of the breast and screening for mutations in exon 2 of MED12 might help to facilitate differential diagnosis between tubular adenoma and fibroadenoma in difficult cases. Lastly, our data exemplarily demonstrate that mutations in cancer-related genes per se do not indicate malignancy but occur in benign tumors. © 2016 Wiley Periodicals, Inc.
AB - Adenomas of the breast are rare benign tumors although single cases with malignant behavior have been reported. However, the genetic basis of these tumors is unknown. Employing targeted next generation sequencing of 50 cancer-related genes as well as Sanger sequencing, we profiled a cohort of 18 mammary adenomas comprising 9 ductal, 6 tubular, and 3 lactating adenoma. Missense mutations were detected in 8 of the 18 cases (44%). Specifically, five (56%) ductal adenomas and three (50%) tubular adenomas harbored mutated genes. No mutations were detected in lactating adenomas. Three of the nine ductal adenomas showed mutant AKT1 (p.E17K) with two of them harboring an additional GNAS mutation (p.R201C). One case had mutant PIK3CA (p.H1047R) and another case a mutation in GNAS (p.R201C). The three cases of mutated tubular adenomas showed mutations in either MET or FGFR3. Of note, we did not detect copy number changes and none of the cases including tubular adenomas had mutations in exon 2 of MED12. Our results suggest that ductal adenomas are related to papillomas of the breast and screening for mutations in exon 2 of MED12 might help to facilitate differential diagnosis between tubular adenoma and fibroadenoma in difficult cases. Lastly, our data exemplarily demonstrate that mutations in cancer-related genes per se do not indicate malignancy but occur in benign tumors. © 2016 Wiley Periodicals, Inc.
KW - Adenoma
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Biomarkers, Tumor
KW - Breast Neoplasms
KW - Chromogranins
KW - Exons
KW - Female
KW - Follow-Up Studies
KW - GTP-Binding Protein alpha Subunits, Gs
KW - High-Throughput Nucleotide Sequencing
KW - Humans
KW - Lactation
KW - Mediator Complex
KW - Middle Aged
KW - Mutation
KW - Neoplasm Staging
KW - Phosphatidylinositol 3-Kinases
KW - Prognosis
KW - Proto-Oncogene Proteins c-akt
KW - Young Adult
KW - Journal Article
U2 - 10.1002/gcc.22396
DO - 10.1002/gcc.22396
M3 - SCORING: Journal article
C2 - 27438523
VL - 56
SP - 11
EP - 17
JO - GENE CHROMOSOME CANC
JF - GENE CHROMOSOME CANC
SN - 1045-2257
IS - 1
ER -