PortalPublikationenTriptolide induces apoptotic death of T lymphocyte

Triptolide induces apoptotic death of T lymphocyte

Standard

Triptolide induces apoptotic death of T lymphocyte. / Yang, Y; Liu, Z; Tolosa, E; Yang, J; Li, L.

in: IMMUNOPHARMACOLOGY, Jahrgang 40, Nr. 2, 01.08.1998, S. 139-49.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Yang, Y, Liu, Z, Tolosa, E, Yang, J & Li, L 1998, 'Triptolide induces apoptotic death of T lymphocyte', IMMUNOPHARMACOLOGY, Jg. 40, Nr. 2, S. 139-49.

APA

Yang, Y., Liu, Z., Tolosa, E., Yang, J., & Li, L. (1998). Triptolide induces apoptotic death of T lymphocyte. IMMUNOPHARMACOLOGY, 40(2), 139-49.

Vancouver

Yang Y, Liu Z, Tolosa E, Yang J, Li L. Triptolide induces apoptotic death of T lymphocyte. IMMUNOPHARMACOLOGY. 1998 Aug 1;40(2):139-49.

Bibtex

@article{304d3bdbece74796b049821ad71b272b,
title = "Triptolide induces apoptotic death of T lymphocyte",
abstract = "Extract of Tripterygium wilfordii Hook. f (TWHf) has immunosuppressive activity and has been used as anti-inflammatory agent in traditional Chinese medicine for centuries. Recent studies have demonstrated that triptolide is the major active component in the extract that inhibits antigen or mitogen-induced T cell proliferation. In attempting to investigate its effect on activation of T lymphocytes, we found triptolide induces apoptotic death of T cell hybridomas and peripheral T cells but not that of thymocytes. The triptolide-induced apoptosis is accompanied by increase of DEVD-cleavable caspases activity and degradation of caspase substrate poly (ADP-ribose) polymerase (PARP). A specific inhibitor of caspases, zVAD-FMK, prevents triptolide-induced PARP degradation and DNA fragmentation but not growth arrest. Furthermore, enforced expression of Bcl-2 inhibited triptolide-induced degradation of PARP and apoptosis. These results indicate that triptolide induces T cell apoptosis through activating caspases, and suggest the growth arrest and apoptotic effect of triptolide may contribute to the immunosuppressive activity of TWHf extract.",
keywords = "Animals, Apoptosis, Caspases, Cell Division, Diterpenes, Enzyme Activation, Epoxy Compounds, Humans, Immunosuppressive Agents, Interleukin-2, Lymphocyte Activation, Mice, Phenanthrenes, Proto-Oncogene Proteins c-bcl-2, Proto-Oncogene Proteins c-fos, T-Lymphocytes",
author = "Y Yang and Z Liu and E Tolosa and J Yang and L Li",
year = "1998",
month = aug,
day = "1",
language = "English",
volume = "40",
pages = "139--49",
journal = "IMMUNOPHARMACOLOGY",
issn = "0162-3109",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Triptolide induces apoptotic death of T lymphocyte

AU - Yang, Y

AU - Liu, Z

AU - Tolosa, E

AU - Yang, J

AU - Li, L

PY - 1998/8/1

Y1 - 1998/8/1

N2 - Extract of Tripterygium wilfordii Hook. f (TWHf) has immunosuppressive activity and has been used as anti-inflammatory agent in traditional Chinese medicine for centuries. Recent studies have demonstrated that triptolide is the major active component in the extract that inhibits antigen or mitogen-induced T cell proliferation. In attempting to investigate its effect on activation of T lymphocytes, we found triptolide induces apoptotic death of T cell hybridomas and peripheral T cells but not that of thymocytes. The triptolide-induced apoptosis is accompanied by increase of DEVD-cleavable caspases activity and degradation of caspase substrate poly (ADP-ribose) polymerase (PARP). A specific inhibitor of caspases, zVAD-FMK, prevents triptolide-induced PARP degradation and DNA fragmentation but not growth arrest. Furthermore, enforced expression of Bcl-2 inhibited triptolide-induced degradation of PARP and apoptosis. These results indicate that triptolide induces T cell apoptosis through activating caspases, and suggest the growth arrest and apoptotic effect of triptolide may contribute to the immunosuppressive activity of TWHf extract.

AB - Extract of Tripterygium wilfordii Hook. f (TWHf) has immunosuppressive activity and has been used as anti-inflammatory agent in traditional Chinese medicine for centuries. Recent studies have demonstrated that triptolide is the major active component in the extract that inhibits antigen or mitogen-induced T cell proliferation. In attempting to investigate its effect on activation of T lymphocytes, we found triptolide induces apoptotic death of T cell hybridomas and peripheral T cells but not that of thymocytes. The triptolide-induced apoptosis is accompanied by increase of DEVD-cleavable caspases activity and degradation of caspase substrate poly (ADP-ribose) polymerase (PARP). A specific inhibitor of caspases, zVAD-FMK, prevents triptolide-induced PARP degradation and DNA fragmentation but not growth arrest. Furthermore, enforced expression of Bcl-2 inhibited triptolide-induced degradation of PARP and apoptosis. These results indicate that triptolide induces T cell apoptosis through activating caspases, and suggest the growth arrest and apoptotic effect of triptolide may contribute to the immunosuppressive activity of TWHf extract.

KW - Animals

KW - Apoptosis

KW - Caspases

KW - Cell Division

KW - Diterpenes

KW - Enzyme Activation

KW - Epoxy Compounds

KW - Humans

KW - Immunosuppressive Agents

KW - Interleukin-2

KW - Lymphocyte Activation

KW - Mice

KW - Phenanthrenes

KW - Proto-Oncogene Proteins c-bcl-2

KW - Proto-Oncogene Proteins c-fos

KW - T-Lymphocytes

M3 - SCORING: Journal article

C2 - 9826028

VL - 40

SP - 139

EP - 149

JO - IMMUNOPHARMACOLOGY

JF - IMMUNOPHARMACOLOGY

SN - 0162-3109

IS - 2

ER -