PortalPublikationenTranscription factor CCAAT/enhancer-binding protein alpha an...

Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma

Standard

Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma. / Thoennissen, Nils H; Thoennissen, Gabriela B; Abbassi, Sam; Nabavi-Nouis, Shayan; Sauer, Tim; Doan, Ngan B; Gery, Sigal; Müller-Tidow, Carsten; Said, Jonathan W; Koeffler, H Phillip.

in: LEUKEMIA LYMPHOMA, Jahrgang 53, Nr. 8, 08.2012, S. 1577-85.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Thoennissen, NH, Thoennissen, GB, Abbassi, S, Nabavi-Nouis, S, Sauer, T, Doan, NB, Gery, S, Müller-Tidow, C, Said, JW & Koeffler, HP 2012, 'Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma', LEUKEMIA LYMPHOMA, Jg. 53, Nr. 8, S. 1577-85. https://doi.org/10.3109/10428194.2012.658792

APA

Thoennissen, N. H., Thoennissen, G. B., Abbassi, S., Nabavi-Nouis, S., Sauer, T., Doan, N. B., Gery, S., Müller-Tidow, C., Said, J. W., & Koeffler, H. P. (2012). Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma. LEUKEMIA LYMPHOMA, 53(8), 1577-85. https://doi.org/10.3109/10428194.2012.658792

Vancouver

Thoennissen NH, Thoennissen GB, Abbassi S, Nabavi-Nouis S, Sauer T, Doan NB et al. Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma. LEUKEMIA LYMPHOMA. 2012 Aug;53(8):1577-85. https://doi.org/10.3109/10428194.2012.658792

Bibtex

@article{7369c7cf9d9d4c5c9760e08ae9312498,
title = "Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma",
abstract = "Disturbances of circadian rhythms and mammalian clock genes have been implicated in the etiologies of many chronic illnesses, including cancer. We show that transcription factor CCAAT/enhancer-binding protein alpha (C/EBPalpha)-regulated PER2 activation is a potential tumor suppressor pathway in diffuse large B-cell lymphoma (DLBCL), one of the commonest types of mature B-cell lymphoma. Expression analysis of human B-cell lymphoma samples including DLBCL (n = 50), mantle cell (n = 21), follicular (n = 25) and Burkitt (n = 18) lymphoma revealed markedly down-regulated CEBPA and PER2 mRNA levels exclusively in DLBCL samples compared to control lymphatic tissue. We demonstrated direct regulation of the circadian core clock gene PER2 by C/EBPalpha in the pro-B cell line Ba/F3, and forced expression of PER2 resulted in decreased proliferation, G0/G1 cell cycle arrest and increased rates of apoptosis. Interestingly, treatment of human DLBCL cell lines with the histone deacetylase-inhibitor suberoylanilide hydroxamic acid (SAHA) significantly increased the expression of C/EBPalpha and Per2, accompanied by cell growth inhibition; in contrast, siRNA knockdown of CEBPA reduced the anti-proliferative effect of SAHA treatment. Our results show for the first time that C/EBPalpha with its associated direct core clock gene target, PER2, are highly deregulated in DLBCL, suggesting an important tumor suppressive pathway in the pathogenesis of this lymphoma entity.",
keywords = "Apoptosis, Biopsy, CCAAT-Enhancer-Binding Protein-alpha, Cell Cycle, Cell Line, Tumor, Cell Proliferation, Cell Survival, Circadian Rhythm, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genes, Reporter, Humans, Lymphoma, Large B-Cell, Diffuse, Models, Genetic, Period Circadian Proteins",
author = "Thoennissen, {Nils H} and Thoennissen, {Gabriela B} and Sam Abbassi and Shayan Nabavi-Nouis and Tim Sauer and Doan, {Ngan B} and Sigal Gery and Carsten M{\"u}ller-Tidow and Said, {Jonathan W} and Koeffler, {H Phillip}",
year = "2012",
month = aug,
doi = "10.3109/10428194.2012.658792",
language = "English",
volume = "53",
pages = "1577--85",
journal = "LEUKEMIA LYMPHOMA",
issn = "1042-8194",
publisher = "informa healthcare",
number = "8",

}

RIS

TY - JOUR

T1 - Transcription factor CCAAT/enhancer-binding protein alpha and critical circadian clock downstream target gene PER2 are highly deregulated in diffuse large B-cell lymphoma

AU - Thoennissen, Nils H

AU - Thoennissen, Gabriela B

AU - Abbassi, Sam

AU - Nabavi-Nouis, Shayan

AU - Sauer, Tim

AU - Doan, Ngan B

AU - Gery, Sigal

AU - Müller-Tidow, Carsten

AU - Said, Jonathan W

AU - Koeffler, H Phillip

PY - 2012/8

Y1 - 2012/8

N2 - Disturbances of circadian rhythms and mammalian clock genes have been implicated in the etiologies of many chronic illnesses, including cancer. We show that transcription factor CCAAT/enhancer-binding protein alpha (C/EBPalpha)-regulated PER2 activation is a potential tumor suppressor pathway in diffuse large B-cell lymphoma (DLBCL), one of the commonest types of mature B-cell lymphoma. Expression analysis of human B-cell lymphoma samples including DLBCL (n = 50), mantle cell (n = 21), follicular (n = 25) and Burkitt (n = 18) lymphoma revealed markedly down-regulated CEBPA and PER2 mRNA levels exclusively in DLBCL samples compared to control lymphatic tissue. We demonstrated direct regulation of the circadian core clock gene PER2 by C/EBPalpha in the pro-B cell line Ba/F3, and forced expression of PER2 resulted in decreased proliferation, G0/G1 cell cycle arrest and increased rates of apoptosis. Interestingly, treatment of human DLBCL cell lines with the histone deacetylase-inhibitor suberoylanilide hydroxamic acid (SAHA) significantly increased the expression of C/EBPalpha and Per2, accompanied by cell growth inhibition; in contrast, siRNA knockdown of CEBPA reduced the anti-proliferative effect of SAHA treatment. Our results show for the first time that C/EBPalpha with its associated direct core clock gene target, PER2, are highly deregulated in DLBCL, suggesting an important tumor suppressive pathway in the pathogenesis of this lymphoma entity.

AB - Disturbances of circadian rhythms and mammalian clock genes have been implicated in the etiologies of many chronic illnesses, including cancer. We show that transcription factor CCAAT/enhancer-binding protein alpha (C/EBPalpha)-regulated PER2 activation is a potential tumor suppressor pathway in diffuse large B-cell lymphoma (DLBCL), one of the commonest types of mature B-cell lymphoma. Expression analysis of human B-cell lymphoma samples including DLBCL (n = 50), mantle cell (n = 21), follicular (n = 25) and Burkitt (n = 18) lymphoma revealed markedly down-regulated CEBPA and PER2 mRNA levels exclusively in DLBCL samples compared to control lymphatic tissue. We demonstrated direct regulation of the circadian core clock gene PER2 by C/EBPalpha in the pro-B cell line Ba/F3, and forced expression of PER2 resulted in decreased proliferation, G0/G1 cell cycle arrest and increased rates of apoptosis. Interestingly, treatment of human DLBCL cell lines with the histone deacetylase-inhibitor suberoylanilide hydroxamic acid (SAHA) significantly increased the expression of C/EBPalpha and Per2, accompanied by cell growth inhibition; in contrast, siRNA knockdown of CEBPA reduced the anti-proliferative effect of SAHA treatment. Our results show for the first time that C/EBPalpha with its associated direct core clock gene target, PER2, are highly deregulated in DLBCL, suggesting an important tumor suppressive pathway in the pathogenesis of this lymphoma entity.

KW - Apoptosis

KW - Biopsy

KW - CCAAT-Enhancer-Binding Protein-alpha

KW - Cell Cycle

KW - Cell Line, Tumor

KW - Cell Proliferation

KW - Cell Survival

KW - Circadian Rhythm

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Genes, Reporter

KW - Humans

KW - Lymphoma, Large B-Cell, Diffuse

KW - Models, Genetic

KW - Period Circadian Proteins

U2 - 10.3109/10428194.2012.658792

DO - 10.3109/10428194.2012.658792

M3 - SCORING: Journal article

C2 - 22260161

VL - 53

SP - 1577

EP - 1585

JO - LEUKEMIA LYMPHOMA

JF - LEUKEMIA LYMPHOMA

SN - 1042-8194

IS - 8

ER -