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Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI

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Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI. / Weill, Uri; Arakel, Eric C; Goldmann, Omer; Golan, Matan; Chuartzman, Silvia; Munro, Sean; Schwappach, Blanche; Schuldiner, Maya.

in: TRAFFIC, Jahrgang 19, Nr. 5, 05.2018, S. 370-379.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Weill, U, Arakel, EC, Goldmann, O, Golan, M, Chuartzman, S, Munro, S, Schwappach, B & Schuldiner, M 2018, 'Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI', TRAFFIC, Jg. 19, Nr. 5, S. 370-379. https://doi.org/10.1111/tra.12560

APA

Weill, U., Arakel, E. C., Goldmann, O., Golan, M., Chuartzman, S., Munro, S., Schwappach, B., & Schuldiner, M. (2018). Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI. TRAFFIC, 19(5), 370-379. https://doi.org/10.1111/tra.12560

Vancouver

Weill U, Arakel EC, Goldmann O, Golan M, Chuartzman S, Munro S et al. Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI. TRAFFIC. 2018 Mai;19(5):370-379. https://doi.org/10.1111/tra.12560

Bibtex

@article{f0dfa2da815c4dd7af58018a0e8f3b9e,
title = "Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI",
abstract = "A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.",
keywords = "Coat Protein Complex I/metabolism, Databases, Protein, Golgi Apparatus/metabolism, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins/metabolism, Secretory Pathway",
author = "Uri Weill and Arakel, {Eric C} and Omer Goldmann and Matan Golan and Silvia Chuartzman and Sean Munro and Blanche Schwappach and Maya Schuldiner",
note = "{\textcopyright} 2018 The Authors. Traffic published by John Wiley & Sons Ltd.",
year = "2018",
month = may,
doi = "10.1111/tra.12560",
language = "English",
volume = "19",
pages = "370--379",
journal = "TRAFFIC",
issn = "1398-9219",
publisher = "Blackwell Munksgaard",
number = "5",

}

RIS

TY - JOUR

T1 - Toolbox: Creating a systematic database of secretory pathway proteins uncovers new cargo for COPI

AU - Weill, Uri

AU - Arakel, Eric C

AU - Goldmann, Omer

AU - Golan, Matan

AU - Chuartzman, Silvia

AU - Munro, Sean

AU - Schwappach, Blanche

AU - Schuldiner, Maya

N1 - © 2018 The Authors. Traffic published by John Wiley & Sons Ltd.

PY - 2018/5

Y1 - 2018/5

N2 - A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.

AB - A third of yeast genes encode for proteins that function in the endomembrane system. However, the precise localization for many of these proteins is still uncertain. Here, we visualized a collection of ~500 N-terminally, green fluorescent protein (GFP), tagged proteins of the yeast Saccharomyces cerevisiae. By co-localizing them with 7 known markers of endomembrane compartments we determined the localization for over 200 of them. Using this approach, we create a systematic database of the various secretory compartments and identify several new residents. Focusing in, we now suggest that Lam5 resides in contact sites between the endoplasmic reticulum and the late Golgi. Additionally, analysis of interactions between the COPI coat and co-localizing proteins from our screen identifies a subset of proteins that are COPI-cargo. In summary, our approach defines the protein roster within each compartment enabling characterization of the physical and functional organization of the endomembrane system and its components.

KW - Coat Protein Complex I/metabolism

KW - Databases, Protein

KW - Golgi Apparatus/metabolism

KW - Protein Transport

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins/metabolism

KW - Secretory Pathway

U2 - 10.1111/tra.12560

DO - 10.1111/tra.12560

M3 - SCORING: Journal article

C2 - 29527758

VL - 19

SP - 370

EP - 379

JO - TRAFFIC

JF - TRAFFIC

SN - 1398-9219

IS - 5

ER -