Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro
Beteiligte Einrichtungen
Abstract
OBJECTIVES: To explore the potential application of B-OT in the aspiration tract.
MATERIALS AND METHODS: We conceived and optimized an in vitro model simulating the mouth-washing process to assess tolerance to B-OT on primary human gingival fibroblasts. Cells derived from 4 unrelated donors were flushed with medium containing drugs of various concentration for one minute twice daily for 3 days.
RESULTS: No effect was seen on the cells up to 1000 µM B-OT. In addition, we treated the cells with B-OT permanently in medium, corresponding to a systemic treatment. No effect was seen by 10 µM B-OT and only a slight reduction (approximately 10%) was seen by 100 µM B-OT.
CONCLUSIONS: Our results suggest good tolerance of oral cells for B-OT, favoring the further development of this antiviral reagent as a mouth-washing solution and nasal spray.
Bibliografische Daten
Originalsprache | Englisch |
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Aufsatznummer | 4112 |
ISSN | 1661-7827 |
DOIs | |
Status | Veröffentlicht - 30.03.2022 |
PubMed | 35409800 |
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