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Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways

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Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. / Beck, Florian; Geiger, Jörg; Gambaryan, Stepan; Veit, Johannes; Vaudel, Marc; Nollau, Peter; Kohlbacher, Oliver; Martens, Lennart; Walter, Ulrich; Sickmann, Albert; Zahedi, René P.

in: BLOOD, Jahrgang 123, Nr. 5, 30.01.2014, S. e1-e10.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Beck, F, Geiger, J, Gambaryan, S, Veit, J, Vaudel, M, Nollau, P, Kohlbacher, O, Martens, L, Walter, U, Sickmann, A & Zahedi, RP 2014, 'Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways', BLOOD, Jg. 123, Nr. 5, S. e1-e10. https://doi.org/10.1182/blood-2013-07-512384

APA

Beck, F., Geiger, J., Gambaryan, S., Veit, J., Vaudel, M., Nollau, P., Kohlbacher, O., Martens, L., Walter, U., Sickmann, A., & Zahedi, R. P. (2014). Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD, 123(5), e1-e10. https://doi.org/10.1182/blood-2013-07-512384

Vancouver

Beck F, Geiger J, Gambaryan S, Veit J, Vaudel M, Nollau P et al. Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways. BLOOD. 2014 Jan 30;123(5):e1-e10. https://doi.org/10.1182/blood-2013-07-512384

Bibtex

@article{4832d244c968495ba57f4de8668aca2c,
title = "Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways",
abstract = "One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multipronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.",
keywords = "Blood Platelets, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Humans, Iloprost, Phosphorylation, Platelet Activation, Platelet Aggregation Inhibitors, Protein Interaction Maps, Signal Transduction",
author = "Florian Beck and J{\"o}rg Geiger and Stepan Gambaryan and Johannes Veit and Marc Vaudel and Peter Nollau and Oliver Kohlbacher and Lennart Martens and Ulrich Walter and Albert Sickmann and Zahedi, {Ren{\'e} P}",
year = "2014",
month = jan,
day = "30",
doi = "10.1182/blood-2013-07-512384",
language = "English",
volume = "123",
pages = "e1--e10",
journal = "BLOOD",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "5",

}

RIS

TY - JOUR

T1 - Time-resolved characterization of cAMP/PKA-dependent signaling reveals that platelet inhibition is a concerted process involving multiple signaling pathways

AU - Beck, Florian

AU - Geiger, Jörg

AU - Gambaryan, Stepan

AU - Veit, Johannes

AU - Vaudel, Marc

AU - Nollau, Peter

AU - Kohlbacher, Oliver

AU - Martens, Lennart

AU - Walter, Ulrich

AU - Sickmann, Albert

AU - Zahedi, René P

PY - 2014/1/30

Y1 - 2014/1/30

N2 - One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multipronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.

AB - One of the most important physiological platelet inhibitors is endothelium-derived prostacyclin which stimulates the platelet cyclic adenosine monophosphate/protein kinase A (cAMP/PKA)-signaling cascade and inhibits virtually all platelet-activating key mechanisms. Using quantitative mass spectrometry, we analyzed time-resolved phosphorylation patterns in human platelets after treatment with iloprost, a stable prostacyclin analog, for 0, 10, 30, and 60 seconds to characterize key mediators of platelet inhibition and activation in 3 independent biological replicates. We quantified over 2700 different phosphorylated peptides of which 360 were significantly regulated upon stimulation. This comprehensive and time-resolved analysis indicates that platelet inhibition is a multipronged process involving different kinases and phosphatases as well as many previously unanticipated proteins and pathways.

KW - Blood Platelets

KW - Cyclic AMP

KW - Cyclic AMP-Dependent Protein Kinases

KW - Humans

KW - Iloprost

KW - Phosphorylation

KW - Platelet Activation

KW - Platelet Aggregation Inhibitors

KW - Protein Interaction Maps

KW - Signal Transduction

U2 - 10.1182/blood-2013-07-512384

DO - 10.1182/blood-2013-07-512384

M3 - SCORING: Journal article

C2 - 24324209

VL - 123

SP - e1-e10

JO - BLOOD

JF - BLOOD

SN - 0006-4971

IS - 5

ER -