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Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents

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Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents. / Rothe, Jan H; Rudolph, Ines; Rohwer, Nadine; Kupitz, Dennis; Gregor-Mamoudou, Betina; Derlin, Thorsten; Furth, Christian; Amthauer, Holger; Brenner, Winfried; Buchert, Ralph; Cramer, Thorsten; Apostolova, Ivayla.

in: ACAD RADIOL, Jahrgang 22, Nr. 2, 02.2015, S. 169-78.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Rothe, JH, Rudolph, I, Rohwer, N, Kupitz, D, Gregor-Mamoudou, B, Derlin, T, Furth, C, Amthauer, H, Brenner, W, Buchert, R, Cramer, T & Apostolova, I 2015, 'Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents', ACAD RADIOL, Jg. 22, Nr. 2, S. 169-78. https://doi.org/10.1016/j.acra.2014.07.022

APA

Rothe, J. H., Rudolph, I., Rohwer, N., Kupitz, D., Gregor-Mamoudou, B., Derlin, T., Furth, C., Amthauer, H., Brenner, W., Buchert, R., Cramer, T., & Apostolova, I. (2015). Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents. ACAD RADIOL, 22(2), 169-78. https://doi.org/10.1016/j.acra.2014.07.022

Vancouver

Rothe JH, Rudolph I, Rohwer N, Kupitz D, Gregor-Mamoudou B, Derlin T et al. Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents. ACAD RADIOL. 2015 Feb;22(2):169-78. https://doi.org/10.1016/j.acra.2014.07.022

Bibtex

@article{ccac3f674fff4f25b1b9c1c6ccfba9b1,
title = "Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents",
abstract = "RATIONALE AND OBJECTIVES: The aim of the present study was to characterize the kinetics of two nanoparticle-based contrast agents for preclinical imaging, Exitron nano 6000 and Exitron nano 12000, and the iodinated agent eXIA 160 in both healthy mice and in a mouse model of hepatocellular carcinoma (HCC). Semiautomatic segmentation of liver lesions for estimation of total tumor load of the liver was evaluated in HCC mice.MATERIALS AND METHODS: The normal time course of contrast enhancement was assessed in 15 healthy C57BL/6 mice. Imaging of tumor spread in the liver was evaluated in 15 mice harboring a transgenic HCC model (ASV-B mice). Automatic segmentation of liver lesions for determination of total tumor burden of the liver was tested in three additional ASV-B mice before and after an experimental therapy.RESULTS: In healthy mice, clearance of the contrast agent from blood was completed within 3-4 hours for eXIA 160 and Exitron nano 6000, whereas complete blood clearance of Exitron nano 12000 required about 24 hours. eXIA 160 provided maximum liver contrast at 1 hour post injection (p.i.) followed by a continuous decline. Enhancement of liver contrast with Exitron nano 6000 and Exitron nano 12000 reached a plateau at about 4 hours p.i., which lasted until the end of the measurements at 96 hours p.i. Maximum contrast enhancement of the liver was not statistically different between Exitron nano 6000 and Exitron nano 12000, but was about three times lower for eXIA 160 (P < .05). Visually Exitron nano 12000 provided the best liver-to-tumor contrast. Semiautomatic liver and tumor segmentation was feasible after the administration of Exitron nano 12000 but did not work properly for the other two contrast agents.CONCLUSIONS: Both nanoparticle-based contrast agents provided stronger and longer lasting contrast enhancement of healthy liver parenchyma. Exitron nano 12000 allowed automatic segmentation of tumor lesions for estimation of the total tumor load in the liver.",
keywords = "Animals, Carcinoma, Hepatocellular, Computer Simulation, Contrast Media, Image Interpretation, Computer-Assisted, Iodine, Liver Neoplasms, Metabolic Clearance Rate, Mice, Mice, Inbred C57BL, Mice, Transgenic, Models, Biological, Nanoparticles, Reference Values, Reproducibility of Results, Sensitivity and Specificity, Tomography, X-Ray Computed, Comparative Study, Evaluation Studies, Journal Article",
author = "Rothe, {Jan H} and Ines Rudolph and Nadine Rohwer and Dennis Kupitz and Betina Gregor-Mamoudou and Thorsten Derlin and Christian Furth and Holger Amthauer and Winfried Brenner and Ralph Buchert and Thorsten Cramer and Ivayla Apostolova",
note = "Copyright {\textcopyright} 2015 AUR. Published by Elsevier Inc. All rights reserved.",
year = "2015",
month = feb,
doi = "10.1016/j.acra.2014.07.022",
language = "English",
volume = "22",
pages = "169--78",
journal = "ACAD RADIOL",
issn = "1076-6332",
publisher = "Elsevier USA",
number = "2",

}

RIS

TY - JOUR

T1 - Time course of contrast enhancement by micro-CT with dedicated contrast agents in normal mice and mice with hepatocellular carcinoma: comparison of one iodinated and two nanoparticle-based agents

AU - Rothe, Jan H

AU - Rudolph, Ines

AU - Rohwer, Nadine

AU - Kupitz, Dennis

AU - Gregor-Mamoudou, Betina

AU - Derlin, Thorsten

AU - Furth, Christian

AU - Amthauer, Holger

AU - Brenner, Winfried

AU - Buchert, Ralph

AU - Cramer, Thorsten

AU - Apostolova, Ivayla

N1 - Copyright © 2015 AUR. Published by Elsevier Inc. All rights reserved.

PY - 2015/2

Y1 - 2015/2

N2 - RATIONALE AND OBJECTIVES: The aim of the present study was to characterize the kinetics of two nanoparticle-based contrast agents for preclinical imaging, Exitron nano 6000 and Exitron nano 12000, and the iodinated agent eXIA 160 in both healthy mice and in a mouse model of hepatocellular carcinoma (HCC). Semiautomatic segmentation of liver lesions for estimation of total tumor load of the liver was evaluated in HCC mice.MATERIALS AND METHODS: The normal time course of contrast enhancement was assessed in 15 healthy C57BL/6 mice. Imaging of tumor spread in the liver was evaluated in 15 mice harboring a transgenic HCC model (ASV-B mice). Automatic segmentation of liver lesions for determination of total tumor burden of the liver was tested in three additional ASV-B mice before and after an experimental therapy.RESULTS: In healthy mice, clearance of the contrast agent from blood was completed within 3-4 hours for eXIA 160 and Exitron nano 6000, whereas complete blood clearance of Exitron nano 12000 required about 24 hours. eXIA 160 provided maximum liver contrast at 1 hour post injection (p.i.) followed by a continuous decline. Enhancement of liver contrast with Exitron nano 6000 and Exitron nano 12000 reached a plateau at about 4 hours p.i., which lasted until the end of the measurements at 96 hours p.i. Maximum contrast enhancement of the liver was not statistically different between Exitron nano 6000 and Exitron nano 12000, but was about three times lower for eXIA 160 (P < .05). Visually Exitron nano 12000 provided the best liver-to-tumor contrast. Semiautomatic liver and tumor segmentation was feasible after the administration of Exitron nano 12000 but did not work properly for the other two contrast agents.CONCLUSIONS: Both nanoparticle-based contrast agents provided stronger and longer lasting contrast enhancement of healthy liver parenchyma. Exitron nano 12000 allowed automatic segmentation of tumor lesions for estimation of the total tumor load in the liver.

AB - RATIONALE AND OBJECTIVES: The aim of the present study was to characterize the kinetics of two nanoparticle-based contrast agents for preclinical imaging, Exitron nano 6000 and Exitron nano 12000, and the iodinated agent eXIA 160 in both healthy mice and in a mouse model of hepatocellular carcinoma (HCC). Semiautomatic segmentation of liver lesions for estimation of total tumor load of the liver was evaluated in HCC mice.MATERIALS AND METHODS: The normal time course of contrast enhancement was assessed in 15 healthy C57BL/6 mice. Imaging of tumor spread in the liver was evaluated in 15 mice harboring a transgenic HCC model (ASV-B mice). Automatic segmentation of liver lesions for determination of total tumor burden of the liver was tested in three additional ASV-B mice before and after an experimental therapy.RESULTS: In healthy mice, clearance of the contrast agent from blood was completed within 3-4 hours for eXIA 160 and Exitron nano 6000, whereas complete blood clearance of Exitron nano 12000 required about 24 hours. eXIA 160 provided maximum liver contrast at 1 hour post injection (p.i.) followed by a continuous decline. Enhancement of liver contrast with Exitron nano 6000 and Exitron nano 12000 reached a plateau at about 4 hours p.i., which lasted until the end of the measurements at 96 hours p.i. Maximum contrast enhancement of the liver was not statistically different between Exitron nano 6000 and Exitron nano 12000, but was about three times lower for eXIA 160 (P < .05). Visually Exitron nano 12000 provided the best liver-to-tumor contrast. Semiautomatic liver and tumor segmentation was feasible after the administration of Exitron nano 12000 but did not work properly for the other two contrast agents.CONCLUSIONS: Both nanoparticle-based contrast agents provided stronger and longer lasting contrast enhancement of healthy liver parenchyma. Exitron nano 12000 allowed automatic segmentation of tumor lesions for estimation of the total tumor load in the liver.

KW - Animals

KW - Carcinoma, Hepatocellular

KW - Computer Simulation

KW - Contrast Media

KW - Image Interpretation, Computer-Assisted

KW - Iodine

KW - Liver Neoplasms

KW - Metabolic Clearance Rate

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Models, Biological

KW - Nanoparticles

KW - Reference Values

KW - Reproducibility of Results

KW - Sensitivity and Specificity

KW - Tomography, X-Ray Computed

KW - Comparative Study

KW - Evaluation Studies

KW - Journal Article

U2 - 10.1016/j.acra.2014.07.022

DO - 10.1016/j.acra.2014.07.022

M3 - SCORING: Journal article

C2 - 25282584

VL - 22

SP - 169

EP - 178

JO - ACAD RADIOL

JF - ACAD RADIOL

SN - 1076-6332

IS - 2

ER -