Thromboxane synthase regulates the migratory phenotype of human glioma cells

A Giese; C Hagel; E L Kim; S Zapf; J Djawaheri; M E Berens; M Westphal

Thromboxane synthase regulates the migratory phenotype of human glioma cells

Standard

Thromboxane synthase regulates the migratory phenotype of human glioma cells. / Giese, A; Hagel, C; Kim, E L; Zapf, S; Djawaheri, J; Berens, M E; Westphal, M.

in: NEURO-ONCOLOGY, Jahrgang 1, Nr. 1, 01.01.1999, S. 3-13.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Giese, A, Hagel, C, Kim, EL, Zapf, S, Djawaheri, J, Berens, ME & Westphal, M 1999, 'Thromboxane synthase regulates the migratory phenotype of human glioma cells', NEURO-ONCOLOGY, Jg. 1, Nr. 1, S. 3-13.

APA

Giese, A., Hagel, C., Kim, E. L., Zapf, S., Djawaheri, J., Berens, M. E., & Westphal, M. (1999). Thromboxane synthase regulates the migratory phenotype of human glioma cells. NEURO-ONCOLOGY, 1(1), 3-13.

Vancouver

Giese A, Hagel C, Kim EL, Zapf S, Djawaheri J, Berens ME et al. Thromboxane synthase regulates the migratory phenotype of human glioma cells. NEURO-ONCOLOGY. 1999 Jan 1;1(1):3-13.

Bibtex

@article{13d9739326d044d6bab4b7b8f9b00f28,

title = "Thromboxane synthase regulates the migratory phenotype of human glioma cells",

abstract = "The capacity of glial tumor cells to migrate and diffusely infiltrate normal brain compromises surgical eradication of the disease. Identification of genes associated with invasion may offer novel strategies for anti-invasive therapies. The gene for TXsyn, an enzyme of the arachidonic acid pathway, has been identified by differential mRNA display as being overexpressed in a glioma cell line selected for migration. In this study TXsyn mRNA expression was found in a large panel of glioma cell lines but not in a strain of human astrocytes. Immunohistochemistry demonstrated TXsyn in the parenchyma of glial tumors and in reactive astrocytes, whereas it could not be detected in quiescent astrocytes and oligodendroglia of normal brain. Glioma cell lines showed a wide range of thromboxane B2 formation, the relative expression of which correlated with migration rates of these cells. Migration was effectively blocked by specific inhibitors of TXsyn, such as furegrelate and dazmegrel. Other TXsyn inhibitors and cyclooxygenase inhibitors were less effective. Treatment with specific inhibitors also resulted in a decrease of intercellular adhesion in glioma cells. These data indicate that TXsyn plays a crucial role in the signal transduction of migration in glial tumors and may offer a novel strategy for anti-invasive therapies.",

keywords = "Arachidonic Acids, Aspirin, Astrocytes, Benzofurans, Brain Neoplasms, Cell Adhesion, Cell Movement, Enzyme Induction, Enzyme Inhibitors, GTP-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Glioma, Humans, Imidazoles, Indomethacin, Lysine, Models, Biological, Neoplasm Proteins, Neoplastic Stem Cells, Oligodendroglia, Pentanoic Acids, Phenotype, Pyridines, RNA, Messenger, RNA, Neoplasm, Signal Transduction, Thromboxane B2, Thromboxane-A Synthase, Tumor Cells, Cultured",

author = "A Giese and C Hagel and Kim, {E L} and S Zapf and J Djawaheri and Berens, {M E} and M Westphal",

year = "1999",

month = jan,

day = "1",

language = "English",

volume = "1",

pages = "3--13",

journal = "NEURO-ONCOLOGY",

issn = "1522-8517",

publisher = "Oxford University Press",

number = "1",

}

RIS

TY - JOUR

T1 - Thromboxane synthase regulates the migratory phenotype of human glioma cells

AU - Giese, A

AU - Hagel, C

AU - Kim, E L

AU - Zapf, S

AU - Djawaheri, J

AU - Berens, M E

AU - Westphal, M

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The capacity of glial tumor cells to migrate and diffusely infiltrate normal brain compromises surgical eradication of the disease. Identification of genes associated with invasion may offer novel strategies for anti-invasive therapies. The gene for TXsyn, an enzyme of the arachidonic acid pathway, has been identified by differential mRNA display as being overexpressed in a glioma cell line selected for migration. In this study TXsyn mRNA expression was found in a large panel of glioma cell lines but not in a strain of human astrocytes. Immunohistochemistry demonstrated TXsyn in the parenchyma of glial tumors and in reactive astrocytes, whereas it could not be detected in quiescent astrocytes and oligodendroglia of normal brain. Glioma cell lines showed a wide range of thromboxane B2 formation, the relative expression of which correlated with migration rates of these cells. Migration was effectively blocked by specific inhibitors of TXsyn, such as furegrelate and dazmegrel. Other TXsyn inhibitors and cyclooxygenase inhibitors were less effective. Treatment with specific inhibitors also resulted in a decrease of intercellular adhesion in glioma cells. These data indicate that TXsyn plays a crucial role in the signal transduction of migration in glial tumors and may offer a novel strategy for anti-invasive therapies.

AB - The capacity of glial tumor cells to migrate and diffusely infiltrate normal brain compromises surgical eradication of the disease. Identification of genes associated with invasion may offer novel strategies for anti-invasive therapies. The gene for TXsyn, an enzyme of the arachidonic acid pathway, has been identified by differential mRNA display as being overexpressed in a glioma cell line selected for migration. In this study TXsyn mRNA expression was found in a large panel of glioma cell lines but not in a strain of human astrocytes. Immunohistochemistry demonstrated TXsyn in the parenchyma of glial tumors and in reactive astrocytes, whereas it could not be detected in quiescent astrocytes and oligodendroglia of normal brain. Glioma cell lines showed a wide range of thromboxane B2 formation, the relative expression of which correlated with migration rates of these cells. Migration was effectively blocked by specific inhibitors of TXsyn, such as furegrelate and dazmegrel. Other TXsyn inhibitors and cyclooxygenase inhibitors were less effective. Treatment with specific inhibitors also resulted in a decrease of intercellular adhesion in glioma cells. These data indicate that TXsyn plays a crucial role in the signal transduction of migration in glial tumors and may offer a novel strategy for anti-invasive therapies.

KW - Arachidonic Acids

KW - Aspirin

KW - Astrocytes

KW - Benzofurans

KW - Brain Neoplasms

KW - Cell Adhesion

KW - Cell Movement

KW - Enzyme Induction

KW - Enzyme Inhibitors

KW - GTP-Binding Proteins

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Glioma

KW - Humans

KW - Imidazoles

KW - Indomethacin

KW - Lysine

KW - Models, Biological

KW - Neoplasm Proteins

KW - Neoplastic Stem Cells

KW - Oligodendroglia

KW - Pentanoic Acids

KW - Phenotype

KW - Pyridines

KW - RNA, Messenger

KW - RNA, Neoplasm

KW - Signal Transduction

KW - Thromboxane B2

KW - Thromboxane-A Synthase

KW - Tumor Cells, Cultured

M3 - SCORING: Journal article

C2 - 11550298

VL - 1

SP - 3

EP - 13

JO - NEURO-ONCOLOGY

JF - NEURO-ONCOLOGY

SN - 1522-8517

IS - 1

ER -