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Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering

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Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. / Maffioletti, Sara Martina; Sarcar, Shilpita; Henderson, Alexander B H; Mannhardt, Ingra; Pinton, Luca; Moyle, Louise Anne; Steele-Stallard, Heather; Cappellari, Ornella; Wells, Kim E; Ferrari, Giulia; Mitchell, Jamie S; Tyzack, Giulia E; Kotiadis, Vassilios N; Khedr, Moustafa; Ragazzi, Martina; Wang, Weixin; Duchen, Michael R; Patani, Rickie; Zammit, Peter S; Wells, Dominic J; Eschenhagen, Thomas; Tedesco, Francesco Saverio.

in: CELL REP, Jahrgang 23, Nr. 3, 17.04.2018, S. 899-908.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Maffioletti, SM, Sarcar, S, Henderson, ABH, Mannhardt, I, Pinton, L, Moyle, LA, Steele-Stallard, H, Cappellari, O, Wells, KE, Ferrari, G, Mitchell, JS, Tyzack, GE, Kotiadis, VN, Khedr, M, Ragazzi, M, Wang, W, Duchen, MR, Patani, R, Zammit, PS, Wells, DJ, Eschenhagen, T & Tedesco, FS 2018, 'Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering', CELL REP, Jg. 23, Nr. 3, S. 899-908. https://doi.org/10.1016/j.celrep.2018.03.091

APA

Maffioletti, S. M., Sarcar, S., Henderson, A. B. H., Mannhardt, I., Pinton, L., Moyle, L. A., Steele-Stallard, H., Cappellari, O., Wells, K. E., Ferrari, G., Mitchell, J. S., Tyzack, G. E., Kotiadis, V. N., Khedr, M., Ragazzi, M., Wang, W., Duchen, M. R., Patani, R., Zammit, P. S., ... Tedesco, F. S. (2018). Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. CELL REP, 23(3), 899-908. https://doi.org/10.1016/j.celrep.2018.03.091

Vancouver

Maffioletti SM, Sarcar S, Henderson ABH, Mannhardt I, Pinton L, Moyle LA et al. Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering. CELL REP. 2018 Apr 17;23(3):899-908. https://doi.org/10.1016/j.celrep.2018.03.091

Bibtex

@article{8f068fa45a47461786b09a2bc29f8a58,
title = "Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering",
abstract = "Generating human skeletal muscle models is instrumental for investigating muscle pathology and therapy. Here, we report the generation of three-dimensional (3D) artificial skeletal muscle tissue from human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) from patients with Duchenne, limb-girdle, and congenital muscular dystrophies. 3D skeletal myogenic differentiation of pluripotent cells was induced within hydrogels under tension to provide myofiber alignment. Artificial muscles recapitulated characteristics of human skeletal muscle tissue and could be implanted into immunodeficient mice. Pathological cellular hallmarks of incurable forms of severe muscular dystrophy could be modeled with high fidelity using this 3D platform. Finally, we show generation of fully human iPSC-derived, complex, multilineage muscle models containing key isogenic cellular constituents of skeletal muscle, including vascular endothelial cells, pericytes, and motor neurons. These results lay the foundation for a human skeletal muscle organoid-like platform for disease modeling, regenerative medicine, and therapy development.",
keywords = "Journal Article",
author = "Maffioletti, {Sara Martina} and Shilpita Sarcar and Henderson, {Alexander B H} and Ingra Mannhardt and Luca Pinton and Moyle, {Louise Anne} and Heather Steele-Stallard and Ornella Cappellari and Wells, {Kim E} and Giulia Ferrari and Mitchell, {Jamie S} and Tyzack, {Giulia E} and Kotiadis, {Vassilios N} and Moustafa Khedr and Martina Ragazzi and Weixin Wang and Duchen, {Michael R} and Rickie Patani and Zammit, {Peter S} and Wells, {Dominic J} and Thomas Eschenhagen and Tedesco, {Francesco Saverio}",
note = "Copyright {\textcopyright} 2018 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = apr,
day = "17",
doi = "10.1016/j.celrep.2018.03.091",
language = "English",
volume = "23",
pages = "899--908",
journal = "CELL REP",
issn = "2211-1247",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering

AU - Maffioletti, Sara Martina

AU - Sarcar, Shilpita

AU - Henderson, Alexander B H

AU - Mannhardt, Ingra

AU - Pinton, Luca

AU - Moyle, Louise Anne

AU - Steele-Stallard, Heather

AU - Cappellari, Ornella

AU - Wells, Kim E

AU - Ferrari, Giulia

AU - Mitchell, Jamie S

AU - Tyzack, Giulia E

AU - Kotiadis, Vassilios N

AU - Khedr, Moustafa

AU - Ragazzi, Martina

AU - Wang, Weixin

AU - Duchen, Michael R

AU - Patani, Rickie

AU - Zammit, Peter S

AU - Wells, Dominic J

AU - Eschenhagen, Thomas

AU - Tedesco, Francesco Saverio

N1 - Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2018/4/17

Y1 - 2018/4/17

N2 - Generating human skeletal muscle models is instrumental for investigating muscle pathology and therapy. Here, we report the generation of three-dimensional (3D) artificial skeletal muscle tissue from human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) from patients with Duchenne, limb-girdle, and congenital muscular dystrophies. 3D skeletal myogenic differentiation of pluripotent cells was induced within hydrogels under tension to provide myofiber alignment. Artificial muscles recapitulated characteristics of human skeletal muscle tissue and could be implanted into immunodeficient mice. Pathological cellular hallmarks of incurable forms of severe muscular dystrophy could be modeled with high fidelity using this 3D platform. Finally, we show generation of fully human iPSC-derived, complex, multilineage muscle models containing key isogenic cellular constituents of skeletal muscle, including vascular endothelial cells, pericytes, and motor neurons. These results lay the foundation for a human skeletal muscle organoid-like platform for disease modeling, regenerative medicine, and therapy development.

AB - Generating human skeletal muscle models is instrumental for investigating muscle pathology and therapy. Here, we report the generation of three-dimensional (3D) artificial skeletal muscle tissue from human pluripotent stem cells, including induced pluripotent stem cells (iPSCs) from patients with Duchenne, limb-girdle, and congenital muscular dystrophies. 3D skeletal myogenic differentiation of pluripotent cells was induced within hydrogels under tension to provide myofiber alignment. Artificial muscles recapitulated characteristics of human skeletal muscle tissue and could be implanted into immunodeficient mice. Pathological cellular hallmarks of incurable forms of severe muscular dystrophy could be modeled with high fidelity using this 3D platform. Finally, we show generation of fully human iPSC-derived, complex, multilineage muscle models containing key isogenic cellular constituents of skeletal muscle, including vascular endothelial cells, pericytes, and motor neurons. These results lay the foundation for a human skeletal muscle organoid-like platform for disease modeling, regenerative medicine, and therapy development.

KW - Journal Article

U2 - 10.1016/j.celrep.2018.03.091

DO - 10.1016/j.celrep.2018.03.091

M3 - SCORING: Journal article

C2 - 29669293

VL - 23

SP - 899

EP - 908

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 3

ER -