The spatial transcriptomic landscape of the healing mouse intestine following damage
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The spatial transcriptomic landscape of the healing mouse intestine following damage. / Parigi, Sara M; Larsson, Ludvig; Das, Srustidhar; Ramirez Flores, Ricardo O; Frede, Annika; Tripathi, Kumar P; Diaz, Oscar E; Selin, Katja; Morales, Rodrigo A; Luo, Xinxin; Monasterio, Gustavo; Engblom, Camilla; Gagliani, Nicola; Saez-Rodriguez, Julio; Lundeberg, Joakim; Villablanca, Eduardo J.
in: NAT COMMUN, Jahrgang 13, Nr. 1, 828, 11.02.2022.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The spatial transcriptomic landscape of the healing mouse intestine following damage
AU - Parigi, Sara M
AU - Larsson, Ludvig
AU - Das, Srustidhar
AU - Ramirez Flores, Ricardo O
AU - Frede, Annika
AU - Tripathi, Kumar P
AU - Diaz, Oscar E
AU - Selin, Katja
AU - Morales, Rodrigo A
AU - Luo, Xinxin
AU - Monasterio, Gustavo
AU - Engblom, Camilla
AU - Gagliani, Nicola
AU - Saez-Rodriguez, Julio
AU - Lundeberg, Joakim
AU - Villablanca, Eduardo J
N1 - © 2022. The Author(s).
PY - 2022/2/11
Y1 - 2022/2/11
N2 - The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
AB - The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
KW - Animals
KW - Colon/metabolism
KW - Disease Models, Animal
KW - Epithelial Cells
KW - Female
KW - Intestinal Mucosa/metabolism
KW - Intestines/metabolism
KW - Mice
KW - Mice, Inbred C57BL
KW - Mice, Neurologic Mutants
KW - Signal Transduction
KW - Transcriptome
KW - Wound Healing
U2 - 10.1038/s41467-022-28497-0
DO - 10.1038/s41467-022-28497-0
M3 - SCORING: Journal article
C2 - 35149721
VL - 13
JO - NAT COMMUN
JF - NAT COMMUN
SN - 2041-1723
IS - 1
M1 - 828
ER -