The assessment of laryngeal pathology and voice dysfunction requires knowledge of anatomy and physiology as well as thorough understanding of the biomechanical (non)linear behaviour of vocal fold vibration. Videostroboscopy adds useful information to voice assessment, which always includes perceptual voice assessment and endoscopy of the larynx. Videostroboscopy can help in differentiating between benign vocal fold lesions, invasive processes, vocal fold scarring and functional disorders. Videostroboscopic evaluation can be improved by using different endoscopes and specific evaluation manoeuvres.