The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system
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The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system. / Schaeren-Wiemers, Nicole; Bonnet, Annick; Erb, Michael; Erne, Beat; Bartsch, Udo; Kern, Frances; Mantei, Ned; Sherman, Diane; Suter, Ueli.
in: J CELL BIOL, Jahrgang 166, Nr. 5, 30.08.2004, S. 731-42.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - The raft-associated protein MAL is required for maintenance of proper axon--glia interactions in the central nervous system
AU - Schaeren-Wiemers, Nicole
AU - Bonnet, Annick
AU - Erb, Michael
AU - Erne, Beat
AU - Bartsch, Udo
AU - Kern, Frances
AU - Mantei, Ned
AU - Sherman, Diane
AU - Suter, Ueli
PY - 2004/8/30
Y1 - 2004/8/30
N2 - The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and disorganized transverse bands at the paranode--axon interface in the adult central nervous system. These structural changes were accompanied by a marked reduction of contactin-associated protein/paranodin, neurofascin 155 (NF155), and the potassium channel Kv1.2, whereas nodal clusters of sodium channels were unaltered. Initial formation of paranodal regions appeared normal, but abnormalities became detectable when MAL started to be expressed. Biochemical analysis revealed reduced myelin-associated glycoprotein, myelin basic protein, and NF155 protein levels in myelin and myelin-derived rafts. Our results demonstrate a critical role for MAL in the maintenance of central nervous system paranodes, likely by controlling the trafficking and/or sorting of NF155 and other membrane components in oligodendrocytes.
AB - The myelin and lymphocyte protein (MAL) is a tetraspan raft-associated proteolipid predominantly expressed by oligodendrocytes and Schwann cells. We show that genetic ablation of mal resulted in cytoplasmic inclusions within compact myelin, paranodal loops that are everted away from the axon, and disorganized transverse bands at the paranode--axon interface in the adult central nervous system. These structural changes were accompanied by a marked reduction of contactin-associated protein/paranodin, neurofascin 155 (NF155), and the potassium channel Kv1.2, whereas nodal clusters of sodium channels were unaltered. Initial formation of paranodal regions appeared normal, but abnormalities became detectable when MAL started to be expressed. Biochemical analysis revealed reduced myelin-associated glycoprotein, myelin basic protein, and NF155 protein levels in myelin and myelin-derived rafts. Our results demonstrate a critical role for MAL in the maintenance of central nervous system paranodes, likely by controlling the trafficking and/or sorting of NF155 and other membrane components in oligodendrocytes.
KW - Animals
KW - Axons
KW - Cell Adhesion Molecules
KW - Cell Communication
KW - Central Nervous System
KW - Down-Regulation
KW - Kv1.2 Potassium Channel
KW - Membrane Microdomains
KW - Membrane Transport Proteins
KW - Mice
KW - Mice, Knockout
KW - Microscopy, Electron
KW - Myelin Basic Protein
KW - Myelin Proteins
KW - Myelin Sheath
KW - Myelin and Lymphocyte-Associated Proteolipid Proteins
KW - Myelin-Associated Glycoprotein
KW - Nerve Growth Factors
KW - Neural Conduction
KW - Oligodendroglia
KW - Optic Nerve
KW - Potassium Channels
KW - Potassium Channels, Voltage-Gated
KW - Protein Transport
KW - Proteolipids
KW - Ranvier's Nodes
KW - Sciatic Nerve
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1083/jcb.200406092
DO - 10.1083/jcb.200406092
M3 - SCORING: Journal article
C2 - 15337780
VL - 166
SP - 731
EP - 742
JO - J CELL BIOL
JF - J CELL BIOL
SN - 0021-9525
IS - 5
ER -