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The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs

Standard

The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. / Wolf, Diana; Rippa, Valentina; Mobarec, Juan Carlos; Sauer, Patricia; Adlung, Lorenz; Kolb, Peter; Bischofs, Ilka B.

in: NUCLEIC ACIDS RES, Jahrgang 44, Nr. 5, 18.03.2016, S. 2160-72.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wolf, D, Rippa, V, Mobarec, JC, Sauer, P, Adlung, L, Kolb, P & Bischofs, IB 2016, 'The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs', NUCLEIC ACIDS RES, Jg. 44, Nr. 5, S. 2160-72. https://doi.org/10.1093/nar/gkv1242

APA

Wolf, D., Rippa, V., Mobarec, J. C., Sauer, P., Adlung, L., Kolb, P., & Bischofs, I. B. (2016). The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. NUCLEIC ACIDS RES, 44(5), 2160-72. https://doi.org/10.1093/nar/gkv1242

Vancouver

Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P et al. The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. NUCLEIC ACIDS RES. 2016 Mär 18;44(5):2160-72. https://doi.org/10.1093/nar/gkv1242

Bibtex

@article{bf94bc1df9c64c7a84b478127e29f34f,
title = "The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs",
abstract = "ComA-like transcription factors regulate the quorum response in numerous Gram-positive bacteria. ComA proteins belong to the tetrahelical helix-turn-helix superfamily of transcriptional activators, which bind as homodimers to inverted sequence repeats in the DNA. Here, we report that ComA from Bacillus subtilis recognizes a topologically distinct motif, in which the binding elements form a direct repeat. We provide in vitro and in vivo evidence that the canonical and non-canonical site play an important role in facilitating type I and type II promoter activation, respectively, by interacting with different subunits of RNA polymerase. We furthermore show that there is a variety of contexts in which the non-canonical site can occur and identify new direct target genes that are located within the integrative and conjugative element ICEBs1. We therefore suggest that ComA acts as a multifunctional transcriptional activator and provides a striking example for complexity in protein-DNA interactions that evolved in the context of quorum sensing. ",
keywords = "Bacillus subtilis/genetics, Bacterial Proteins/genetics, Base Sequence, Binding Sites, Cloning, Molecular, DNA-Binding Proteins/genetics, DNA-Directed RNA Polymerases/genetics, Escherichia coli/genetics, Gene Expression Regulation, Bacterial, Inverted Repeat Sequences, Molecular Sequence Data, Nucleotide Motifs, Promoter Regions, Genetic, Protein Binding, Protein Multimerization, Protein Subunits/genetics, Quorum Sensing/genetics, Recombinant Proteins/genetics, Transcriptional Activation",
author = "Diana Wolf and Valentina Rippa and Mobarec, {Juan Carlos} and Patricia Sauer and Lorenz Adlung and Peter Kolb and Bischofs, {Ilka B}",
note = "{\textcopyright} The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2016",
month = mar,
day = "18",
doi = "10.1093/nar/gkv1242",
language = "English",
volume = "44",
pages = "2160--72",
journal = "NUCLEIC ACIDS RES",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs

AU - Wolf, Diana

AU - Rippa, Valentina

AU - Mobarec, Juan Carlos

AU - Sauer, Patricia

AU - Adlung, Lorenz

AU - Kolb, Peter

AU - Bischofs, Ilka B

N1 - © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2016/3/18

Y1 - 2016/3/18

N2 - ComA-like transcription factors regulate the quorum response in numerous Gram-positive bacteria. ComA proteins belong to the tetrahelical helix-turn-helix superfamily of transcriptional activators, which bind as homodimers to inverted sequence repeats in the DNA. Here, we report that ComA from Bacillus subtilis recognizes a topologically distinct motif, in which the binding elements form a direct repeat. We provide in vitro and in vivo evidence that the canonical and non-canonical site play an important role in facilitating type I and type II promoter activation, respectively, by interacting with different subunits of RNA polymerase. We furthermore show that there is a variety of contexts in which the non-canonical site can occur and identify new direct target genes that are located within the integrative and conjugative element ICEBs1. We therefore suggest that ComA acts as a multifunctional transcriptional activator and provides a striking example for complexity in protein-DNA interactions that evolved in the context of quorum sensing.

AB - ComA-like transcription factors regulate the quorum response in numerous Gram-positive bacteria. ComA proteins belong to the tetrahelical helix-turn-helix superfamily of transcriptional activators, which bind as homodimers to inverted sequence repeats in the DNA. Here, we report that ComA from Bacillus subtilis recognizes a topologically distinct motif, in which the binding elements form a direct repeat. We provide in vitro and in vivo evidence that the canonical and non-canonical site play an important role in facilitating type I and type II promoter activation, respectively, by interacting with different subunits of RNA polymerase. We furthermore show that there is a variety of contexts in which the non-canonical site can occur and identify new direct target genes that are located within the integrative and conjugative element ICEBs1. We therefore suggest that ComA acts as a multifunctional transcriptional activator and provides a striking example for complexity in protein-DNA interactions that evolved in the context of quorum sensing.

KW - Bacillus subtilis/genetics

KW - Bacterial Proteins/genetics

KW - Base Sequence

KW - Binding Sites

KW - Cloning, Molecular

KW - DNA-Binding Proteins/genetics

KW - DNA-Directed RNA Polymerases/genetics

KW - Escherichia coli/genetics

KW - Gene Expression Regulation, Bacterial

KW - Inverted Repeat Sequences

KW - Molecular Sequence Data

KW - Nucleotide Motifs

KW - Promoter Regions, Genetic

KW - Protein Binding

KW - Protein Multimerization

KW - Protein Subunits/genetics

KW - Quorum Sensing/genetics

KW - Recombinant Proteins/genetics

KW - Transcriptional Activation

U2 - 10.1093/nar/gkv1242

DO - 10.1093/nar/gkv1242

M3 - SCORING: Journal article

C2 - 26582911

VL - 44

SP - 2160

EP - 2172

JO - NUCLEIC ACIDS RES

JF - NUCLEIC ACIDS RES

SN - 0305-1048

IS - 5

ER -