The opposing roles of estradiol on synaptic protein expression in hippocampal cultures.
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The opposing roles of estradiol on synaptic protein expression in hippocampal cultures. / Fester, Lars; Zhou, Lepu; Voets, Corinna; Ossig, Christiana; Disteldorf, Erik; Peters, Christian Howard; Bläute, Florian; Prange-Kiel, Janine; Dudzinski, Danuta; Jarry, Hubertus; Rune, Gabriele M.
in: PSYCHONEUROENDOCRINO, Jahrgang 34, Nr. 1, 1, 2009, S. 123-129.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - The opposing roles of estradiol on synaptic protein expression in hippocampal cultures.
AU - Fester, Lars
AU - Zhou, Lepu
AU - Voets, Corinna
AU - Ossig, Christiana
AU - Disteldorf, Erik
AU - Peters, Christian Howard
AU - Bläute, Florian
AU - Prange-Kiel, Janine
AU - Dudzinski, Danuta
AU - Jarry, Hubertus
AU - Rune, Gabriele M.
PY - 2009
Y1 - 2009
N2 - Estrogen-induced synaptic plasticity was frequently shown by an increase of spines at apical dendrites of CA1 pyramidal neurons after systemic application of estradiol to ovariectomized rats. Surprisingly, exogenous application of estradiol to hippocampal cultures had no effect on spines and on spine synapses, although quantitative immunohistochemistry revealed an upregulation of spinophilin and of synaptophysin, in these cultures. The role of synaptophysin as a presynaptic marker and of spinophilin as a postsynaptic marker, appears questionable from these discrepancies. In contrast, synaptopodin, a marker protein of "mature" mushroom-shaped spines, was downregulated after treatment of hippocampal cultures with estradiol. Synaptopodin is strongly associated to the spine apparatus, a spine-specific cell organelle, which is present in 80% of all mushroom-shaped spines. Consistently, we found a reduction in the number of spines, containing a spine apparatus in response to estradiol, suggesting that the presence of a spine apparatus in many but not all spines is very likely a result of their dynamic character. In summary, synaptic proteins appear to be regulated by estradiol, independent of its function on spine and spine synapse formation.
AB - Estrogen-induced synaptic plasticity was frequently shown by an increase of spines at apical dendrites of CA1 pyramidal neurons after systemic application of estradiol to ovariectomized rats. Surprisingly, exogenous application of estradiol to hippocampal cultures had no effect on spines and on spine synapses, although quantitative immunohistochemistry revealed an upregulation of spinophilin and of synaptophysin, in these cultures. The role of synaptophysin as a presynaptic marker and of spinophilin as a postsynaptic marker, appears questionable from these discrepancies. In contrast, synaptopodin, a marker protein of "mature" mushroom-shaped spines, was downregulated after treatment of hippocampal cultures with estradiol. Synaptopodin is strongly associated to the spine apparatus, a spine-specific cell organelle, which is present in 80% of all mushroom-shaped spines. Consistently, we found a reduction in the number of spines, containing a spine apparatus in response to estradiol, suggesting that the presence of a spine apparatus in many but not all spines is very likely a result of their dynamic character. In summary, synaptic proteins appear to be regulated by estradiol, independent of its function on spine and spine synapse formation.
M3 - SCORING: Zeitschriftenaufsatz
VL - 34
SP - 123
EP - 129
JO - PSYCHONEUROENDOCRINO
JF - PSYCHONEUROENDOCRINO
SN - 0306-4530
IS - 1
M1 - 1
ER -
