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The Mouse Isolated Perfused Kidney Technique

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The Mouse Isolated Perfused Kidney Technique. / Czogalla, Jan; Schweda, Frank; Loffing, Johannes.

in: JOVE-J VIS EXP, Nr. 117, 17.11.2016.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Czogalla, J, Schweda, F & Loffing, J 2016, 'The Mouse Isolated Perfused Kidney Technique', JOVE-J VIS EXP, Nr. 117. https://doi.org/10.3791/54712

APA

Czogalla, J., Schweda, F., & Loffing, J. (2016). The Mouse Isolated Perfused Kidney Technique. JOVE-J VIS EXP, (117). https://doi.org/10.3791/54712

Vancouver

Czogalla J, Schweda F, Loffing J. The Mouse Isolated Perfused Kidney Technique. JOVE-J VIS EXP. 2016 Nov 17;(117). https://doi.org/10.3791/54712

Bibtex

@article{fb88e2b512404b4ebe6c8a688a063517,
title = "The Mouse Isolated Perfused Kidney Technique",
abstract = "The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered. After the procedure, the kidney can be transplanted or processed for molecular biology, biochemical analysis, or microscopy. This paper describes the perfusate and the surgical technique needed for the ex vivo perfusion of mouse kidneys. Details of the perfusion apparatus are given and data are presented showing the viability of the kidney's preparation: renal blood flow, vascular resistance, and urine data as functional, transmission electron micrographs of different nephron segments as morphological readouts, and western blots of transport proteins of different nephron segments as molecular readout.",
keywords = "Journal Article",
author = "Jan Czogalla and Frank Schweda and Johannes Loffing",
year = "2016",
month = nov,
day = "17",
doi = "10.3791/54712",
language = "English",
journal = "JOVE-J VIS EXP",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "117",

}

RIS

TY - JOUR

T1 - The Mouse Isolated Perfused Kidney Technique

AU - Czogalla, Jan

AU - Schweda, Frank

AU - Loffing, Johannes

PY - 2016/11/17

Y1 - 2016/11/17

N2 - The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered. After the procedure, the kidney can be transplanted or processed for molecular biology, biochemical analysis, or microscopy. This paper describes the perfusate and the surgical technique needed for the ex vivo perfusion of mouse kidneys. Details of the perfusion apparatus are given and data are presented showing the viability of the kidney's preparation: renal blood flow, vascular resistance, and urine data as functional, transmission electron micrographs of different nephron segments as morphological readouts, and western blots of transport proteins of different nephron segments as molecular readout.

AB - The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered. After the procedure, the kidney can be transplanted or processed for molecular biology, biochemical analysis, or microscopy. This paper describes the perfusate and the surgical technique needed for the ex vivo perfusion of mouse kidneys. Details of the perfusion apparatus are given and data are presented showing the viability of the kidney's preparation: renal blood flow, vascular resistance, and urine data as functional, transmission electron micrographs of different nephron segments as morphological readouts, and western blots of transport proteins of different nephron segments as molecular readout.

KW - Journal Article

U2 - 10.3791/54712

DO - 10.3791/54712

M3 - SCORING: Journal article

C2 - 27911373

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 117

ER -